http://biowww.net/ Tue, 26 Aug 2008 12:51:56 +0100 FeedCreator 1.7.2 http://biowww.net/detail-365.html Propidium iodide (Molecular Probes, Eugene, OR) is a membrane-impermeant dye that stains by intercalating into nucleic acid molecules. It binds both DNA and RNA. (David K. Hanzel and Siobhan C. Pickett, Molecular Dynamics) Staining Nucleic Acids with Pr ... biowww http://biowww.net/detail-119.html Protocol: DIG Glycan Differentiation Kit from Roche. ... biowww http://biowww.net/detail-186.html Tri isolation of DNA RNA and Protein by Tri reagents from MRC. Detailed protocol and troubleshooting guide included. ... biowww http://biowww.net/detail-843.html An introduction and protocol on SCGE single cell gel electrophoresis comet assay by Tobie D. Wolfe. (PPT file) SCGE can be used to: - Detect and quantitate DNA damage - Identify apoptotic cells quickly and easily - Follow DNA repair ... biowww http://biowww.net/detail-30.html I have a DNA binding protein(mw 6100), presumed to be a dimer and not to need any cofactors. It suppresses the replication of a certain gene by binding to its binding sequence overlaped by the promoter site of the gene. Its binding DNA sequence is much highly ... biowww http://biowww.net/detail-69.html The goal is to somehow not detect IP Ab on westerns: Situation/example: As a control experiment to detect efficiency of immunoprecipitation of one component in a complex: Antigen-Rabbit Primary Ab -IP'd By ProteinA/G ... ... ... biowww http://biowww.net/detail-70.html Recently the mini-preps I've done using alkaline lysis and phenol extraction have been giving extremely high OD's, making them look brilliant, until I check by restriction digest and agarose gel, to find there's very little plasmid DNA actually in the ... biowww http://biowww.net/detail-71.html There's got to be a reason for adding NaOAc to the aqueous DNA solution BEFORE adding the EtOH. Is this an absolute necessity? I'm doing >1000X precipitations/day of PCR products in 96 well plates, ... ... ... biowww http://biowww.net/detail-115.html What I wish to do is to look at the repertoire of mutations in a specific gene in a population of cells. So ideally I would like to isolate the same DNA fragment from each cell and sequence it. Obviously PCR on the population of cells won't work ... biowww http://biowww.net/detail-151.html If you see faint or no bands on the gel: There was insufficient quantity or concentration of DNA loaded on the gel. Increase the amount of DNA, but don't exceed 50 ng/band. The DNA was degraded. Avoid nuclease contamination. The DNA was electrop ... biowww