Biowww.net RSS feed http://biowww.net/ Fri, 05 Sep 2008 23:10:15 +0100 FeedCreator 1.7.2 A general protocol of transfection mammalian cells with lipofectamine reagent http://biowww.net/detail-366.html Procedure for Transfection of Mammalian Cells. A general protocol of transfection mammalian cells using lipofectamine reagent (by Julie B. Wolf at UMBC). ... biowww Gene transfer protocols http://biowww.net/detail-189.html Gene transfer protocols from Mirus. Protocols included: In Vitro Transfection Reagents TransIT®- LT1, and -LT2 TransIT®-Insecta In Vivo Gene Delivery System TransIT®- In vivo Non-Radioactive Nucleic Acid Labeling Ki ... biowww Generation of recombinant baculoviruses by co-transfection http://biowww.net/detail-343.html A protocol from Bjorkerman lab at CIT. ... biowww Protocol for Transfecting siRNA into Mammalian Cells Using Lipofectamine 2000 http://biowww.net/detail-575.html Invitrogen manual on Transfecting siRNA into Mammalian Cells Using Lipofectamine 2000. Two general guidelines for siRNA transfection in mammalian cells are: 1. transfect cells with siRNA when cells reach 30-50% confluent. 2. Don't use antibiotics in transfe ... biowww Calcium Phosphate Transfection of PC12 Cells http://biowww.net/detail-811.html Calcium Phosphate Transfection protocol for PC12 cell line. (Finkbeiner lab, UCSF) PC12 cell transfection protocol describes in detail the procedure, notes, media and reagent preparation etc. ... biowww Co-transfection and Immunofluorescence Protocol http://biowww.net/detail-1041.html A pdf prootcol from Lahn lab at University of Chicago on co-transfection and three color fluorescent immunostaining with details on co-transfection efficiency optimization and transfection set-up. ... biowww siRNA transfection http://biowww.net/detail-1394.html Some protocols and overviews on siRNA transfection: Stable Transfection of Mammalian Cells with siRNA expression vectors (Ambion) ... biowww Cell transfection troubleshooting guide http://biowww.net/detail-776.html The cell transfection troubleshooting guide include: 1. General cell transfection troubleshooting tips. 2. Cationic lipid reagent troubleshooting. 3. Calcium phosphate transfection troubleshooting. 4. DEAE-dextran transfection troubleshooting. ... biowww DNA and siRNA transfection experiments troubleshooting guide http://biowww.net/detail-1208.html This is a two pages troubleshooting guide (pdf file) on DNA and siRNA transfection into cells. (Invitrogen) ... biowww Transfection assistant for cell transfection http://biowww.net/detail-785.html Transfection Assistant is an interactive tool to help you find the most appropriate combination of cell line and Promega reagent for a successful transfection. These conditions are intended as guidelines only. Transfection conditions must be optimized for indi ... biowww