<?xml version="1.0" encoding="ISO-8859-1"?>
<!-- generator="FeedCreator 1.7.2" -->
<rss version="2.0">
    <channel>
        <title>Biowww.net RSS feed</title>
        <description><![CDATA[BioNews feeds from biowww dot net]]></description>
        <link>http://biowww.net/</link>
        <lastBuildDate>Tue, 02 Dec 2008 15:57:00 +0100</lastBuildDate>
        <generator>FeedCreator 1.7.2</generator>
        <item>
            <title>Protocol for Sequencing Very Difficult Regions</title>
            <link>http://biowww.net/detail-277.html</link>
            <description>Updated 02-14-02 by Ziyun Yao and B.A. Roe in Roe's lab. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Ethanol Precipitation of DNA Cycle Sequencing Reactions in 384-well plates</title>
            <link>http://biowww.net/detail-278.html</link>
            <description>Protocol from Roe's lab.

This clearly is an &quot;old procedure revisited&quot; but is extremely efficient because
1) the amount of resulting nested fragment set is at least 5 to 10 times more than obtained by Sephadex G-50 filtration and
2) the signal strength on  ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Primer Optimisation for Sequencing Reactions</title>
            <link>http://biowww.net/detail-396.html</link>
            <description>This short protocol  describe how to optimize primerfor sequencing (primer dilution, temperature and magnesium concentration determination). Protocol is from Jonathan Flint Lab. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Long distance RT-PCR</title>
            <link>http://biowww.net/detail-1404.html</link>
            <description>Technique tips for long-distance RT-PCR:

1. Extract poly-A mRNA using oligo-(dT)25 dynabeads. Analyze mRNA quality before RT reaction (Northern blot).

2. Perform RT reaction using SuperScriptII or SuperScriptIII reverse transcriptase per manufacturer's i ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>DNA sequencing</title>
            <link>http://biowww.net/detail-1426.html</link>
            <description>DNA sequencing

DNA sequencing reactions involves DNA denaturation, primer annealing and DNA polymerase elongation (DNA replication) stages. The DNA sequencing reaction mix includes DNA plymerase (Taq polymerase), the 4 free nucleotides and trace of dideoxyr ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Interpreting sequencing chromatograms data</title>
            <link>http://biowww.net/detail-616.html</link>
            <description>This is a brief overview on how to interpret your chromatogram and how to recognize what is good, reliable sequence data and what is not. (Roswell Park Cancer Center Sequencing Core Facility). ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>DNA Sequencing basics</title>
            <link>http://biowww.net/detail-617.html</link>
            <description>DNA sequencing basics: overview of principle of DNA sequencing. It is recommended to read through before troubleshoot your sequencing data. (Rosewell park cancer center). ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>DNA Sequencing: Troubleshooting your data</title>
            <link>http://biowww.net/detail-615.html</link>
            <description>Troubleshooting guide on DNA sequencing from Roswell Park Cancer Center.

As mentioned in other sections of this website, the two most common causes for failure to get good or any sequence data for your samples are purity and concentration of your template D ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>DNA sequencing troubleshooting from Rockefeller DNA sequencing center</title>
            <link>http://biowww.net/detail-244.html</link>
            <description>DNA sequencing troubleshooting from Rockefeller DNA sequencing center
		 
------------------------------------------------------------------------
 Top level  Molecular biology  Cloning and sequencing 
------------------------------------------------------ ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>General recommendation for PCR sequencing</title>
            <link>http://biowww.net/detail-190.html</link>
            <description>The most important factor in obtaining high quality sequence data is that your PCR amplification must be clean, robust and specific. One of the best methods of improving PCR specificity and reliability is to use a hot start PCR. This prevents the PCR reaction  ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Troubleshooting DNA sequencing data</title>
            <link>http://biowww.net/detail-220.html</link>
            <description>A detailed DNA sequencing data interpretation and troubleshooting guide (Greenwood molecular biology facility, University of Hawaii)

If your DNA sequencing data have following problems, you should follow the troubleshooting guide:

- No recognizable seque ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>PNACL's DNA Sequencing Trouble Shooting Guide</title>
            <link>http://biowww.net/detail-389.html</link>
            <description>This troubleshooting guid is a compilation of the problems in DNA Sequencing Facility at the University of Leicester.  ...</description>
            <author>biowww</author>
        </item>
    </channel>
</rss>
