http://biowww.net/ Tue, 26 Aug 2008 05:39:42 +0100 FeedCreator 1.7.2 http://biowww.net/detail-749.html MicroRNA and siRNA Cloning Method (Bartel Lab Protocol, MIT). Procedural Outline: 1. Oligo Design 2. ImpA Synthesis 3. Synthesizing Oligos 4. Oligonucleotide Adenylation 5. Small RNA Purification 6. 3' Adaptor Ligation 7. 5' Adaptor Ligation 8. RT-P ... biowww http://biowww.net/detail-748.html Small RNA Northern blots are actually quite easy, once you are set up for it. In getting set up for it. (McManus, M.T., Sharp lab, MIT) ... biowww http://biowww.net/detail-893.html MicroRNA (miRNA) cloning protocol from Dr.Victor Ambros's lab at Dartmouth medical college. Section I RNA ligation of 3` linker. Section II. Gel purification of (RNA-to-3’ linker) ligation product. Section III. RNA ligation of 5’ linker (optional, “Nels ... biowww http://biowww.net/detail-753.html A brief Introduction to micro-RNA from the science-projects.com. ... biowww http://biowww.net/detail-892.html A letter by VICTOR AMBROS et al. published on RNA (2003), 9:277–279. (Victor Ambros lab, Dartmouth college). ABSTRACT MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characterist ... biowww http://biowww.net/detail-756.html It currently features reviews, research papers, news on microRNA. (Ambion) ... biowww http://biowww.net/detail-754.html The miRNA Registry has been established with two broad aims: (Welcome Trus Sanger Institute) to provide miRNA gene hunters with unique names for novel miRNA genes prior to publication of results to provide a searchable database of published miRNAs ... biowww http://biowww.net/detail-758.html It include human and Drosophila microRNA targets. (www.microrna.org) ... biowww http://biowww.net/detail-1262.html Reference: RNA. 2005 Dec 22; TarBase: A comprehensive database of experimentally supported animal microRNA targets Sethupathy P, Corda B, Hatzigeorgiou AG. MicroRNAs (miRNAs) are ~22-nt RNA segments that are involved in the regulation of prote ... biowww http://biowww.net/detail-757.html miRanda is an algorithm for finding genomic targets for microRNAs. This algorithm has been written in C and is available as an open-source method under the GPL. MiRanda was developed at the Computational Biology Center of Memorial Sloan-Kettering Cancer Center ... biowww http://biowww.net/detail-1172.html If you enter the sequences of two hairpins, MiRscan will assign them a score, based on their similarity to 50 pairs of experimentally verified C. elegans/C. briggsae microRNA hairpins. (MIT) References: Lim, L. P.*, Lau, N. C.*, Weinstein, E. G.*, Abdelh ... biowww http://biowww.net/detail-1173.html MiRAlign is a microRNA identification tool based on sequence and structure alignments. (Tsinghua University, Beijing, China) Reference: Xiaowo Wang, Jing Zhang, Fei Li, Jin Gu, Tao He, Xuegong Zhang, and Yanda Li. MicroRNA identification based on seque ... biowww