http://biowww.net/ Wed, 20 Aug 2008 02:52:04 +0100 FeedCreator 1.7.2 http://biowww.net/detail-1316.html An extremly useful immunohistochemistry staining protocols by The Diabetes and Endocrinology Research Center (DERC) at the University of Colorado Health Sciences Center in Denver, Colorado. (27 pages PDF) ... biowww http://biowww.net/detail-354.html Protocol on immunohistochemistry with whole mouse embryos from Sato's lab at University Texas Southwestern medical center at Dallas. The PDF version of the protocol can be downloaded from the above page. ... biowww http://biowww.net/detail-356.html From Sato's lab at University of Texas Southwestern medical center. Staining procedure for paraffin sections with antibodies to laminin, fibronectin, collagen type IV. PDF version of the protocol is available on the web page. ... biowww http://biowww.net/detail-409.html This protocol is used for for either Purified or Biotinylated primary antibodies for immunohistochemical staining of mouse frozen tissue sections. (ebioscience inc.) ... biowww http://biowww.net/detail-464.html By Constance Cepko et al at Cepko lab, Harvard medical school. ... biowww http://biowww.net/detail-655.html Protocol from Chemicon. Tissue sections (5-7 ¦Ìm thick) are cut from paraffin-embedded blocks on a microtome and mounted from warm water (40¡ãC) onto adhesive microscope slides. Sections are allowed to dry overnight at room temperature or 40¡ãC ... ... ... biowww http://biowww.net/detail-672.html Procedure: First stain HRP Developing solution Second stain AP Developing solution Protocol by Giorgio Cattoretti, Columbia University. ... biowww http://biowww.net/detail-804.html Recipe and protocol for making Fluorescence Mounting Medium (Antifade). From Spector's lab at Cold Spring Harbor Lab. ... biowww http://biowww.net/detail-805.html Short description on how to clean coverslips for immunofluorescent staining. Spector lab at Cold Spring Harbor Lab. ... biowww http://biowww.net/detail-807.html Triple Labeling Immunofluorescence Protocols for Tissue Sections and Cell Cultures. By Ian Jones at University of Bath, UK. ... biowww http://biowww.net/detail-808.html Tissue immunolabelling protocol using ABC/DAB reaction. By Ian Jones at University of Bath, UK. ... biowww http://biowww.net/detail-809.html Immunocytochemistry on free-floating sections protocol by Ann Marie Johnson, Fisher Scientific. ... biowww http://biowww.net/detail-810.html Staining method for cell death: necrosis and apoptosis based on morphology after staining. (Finkbeiner Lab, UCSF) ... biowww http://biowww.net/detail-980.html A pdf general protocol on immunofluorescence staining including antigen retrieval methods. Protocol from Rosen lab, Baylor college of medicine. ... biowww http://biowww.net/detail-1130.html This step is essential for fine structure preservation as well as for producing enough contrast to visualize subcellular detail in the electron microscope. (Eye Hearing Institute) ... biowww http://biowww.net/detail-1154.html Protocol for enzymatic stain frozen cells and tissues, as well as paraffin-embedded tissues. (R&D Systems) Contents: Overview Materials Sample Preparation Tissue Fixation and Mounting - Cryostat Sections Tissue Fixation and Mounting - Para ... biowww http://biowww.net/detail-1311.html A brief protocol introducing double staining fluorescent simultaneous protocol from ABCam inc. A. Cell lines, cytology smears, cytospin preparations. B. Frozen (cryostat) sections. C. Paraffin-embedded sections. ... biowww http://biowww.net/detail-981.html A nice pdf tutorial with detailed instruction and images on general immunostaining procedure written by Sandy Grimm, PhD from Rosen lab at Baylor college of medicine. This immunostaining protocol is for paraffin embedded tissue sections. ... biowww http://biowww.net/detail-176.html Placed on WWW by: Michael Serfas General Note: Protocols for immunohistochemistry vary widely, due to the differences between antigens and their recognition by antibody. Some epitopes are destroyed by the high temperatures and organic solvents used in ... biowww http://biowww.net/detail-410.html Protocols and recipes about antibody staining, antigen retrieval, blocking solutions, antibody dilution buffers, washing buffers, chromagen substrate solutions, counterstain solutions, special stains, TUNEL staining, general IHC protocols, general histology an ... biowww http://biowww.net/detail-79.html A step by step immunostaining protocol with video demonstration on how to perform immunocytochemistry fluorescent staining on cell cultures on coverslips. You are recommended to first download their detailed ... biowww http://biowww.net/detail-427.html Troubleshooting guide on weak staining, overstaining or high background in immunohiostology staining (IHCWorld.com) ... biowww http://biowww.net/detail-1189.html Troubleshoot problems : Lack of Staining, overstaining and high background. (from R&D systems) ... biowww http://biowww.net/detail-1312.html Some technical suggestions for the novice or not so novice user regarding a variety of issues related to confocal and multi-photon imaging. (Confocal Microscopy Core Facility at Brigham and Women's Hospital, Havard University) ... biowww http://biowww.net/detail-31.html I am currently developing a protocol for immunofluorescent detection of the stress proteins hsp70 and cpn60 on cryosectioned Mytilus mantle tissue. The problem I am having is nonspecific binding of the secondary antibody (conjugated to fluorescein), l ... biowww http://biowww.net/detail-46.html I wish to stain fixed cells for two different poteins. I have selective antibodies to each and one which recognises both. Unfortunately, they ae all raised in rabbit. Is there a simple way of labelling the antibodies directly, so that I could double-s ... biowww http://biowww.net/detail-48.html Anyone know how to reduce/abolish autofluorescence on formain fixed, paraffin embedded sections of human endometrium? (Obviously dewaxed before use, but which give a high fluorescent background never having seen any antibody). ... biowww http://biowww.net/detail-51.html Currently I am examining living cells with Green Fluorescent Protein and I want to combine this with a red fluorescent dye to visualize DNA in vital cells using confocal laser scanning microscopy. I cannot use Hoechst or other dyes with UV excitation, ... biowww http://biowww.net/detail-55.html I understand that GFP has become popular of late. However, I'd like to know how a researcher understands what filter sets and flourochromes are required to view a speciman optimally. If I have a filter set at my disposal, how can I find out specificall ... biowww http://biowww.net/detail-778.html Results obtained from immunocytochemical labeling can often differ from what was expected. Sometimes this may result in the formulation of new theories but often good results are ignored because of faulty interpretations. (House Ear Institute) ... biowww http://biowww.net/detail-391.html Developed by Dennis M. Frisman. The database allows histologist 1) list the antibodies that can differentiate between tumors entered by the user (e.g., lung adenocarcinoma vs. breast carcinoma), 2) rank the antibodies in terms of their ability to differentiat ... biowww