http://biowww.net/ Thu, 03 May 2012 20:58:31 +0100 FeedCreator 1.7.2 http://biowww.net/detail-1424.html Ligation of PCR products Materials: • Invitrogen TA cloning kit • LB Liquid Medium (500 ml) : Autoclave, store at 4 C. Tryptone 5 g Yeast extract 2.5 g NaCl 5 g • LB Agar Plate (500 ml) : Autoclave, store at 4 C. Tryptone 5 g Yeast extract ... biowww http://biowww.net/detail-1425.html Low melting agarose DNA ligation protocol 2x Bacteriophage T4 DNA ligase mixture Restriction endonucleases Protocol: - digest target DNA in EP tube (~250 ng of fragment in 20ul volume). - run low-melting-temperature agarose gel to separate the dig ... biowww