http://biowww.net/ Wed, 03 Dec 2008 22:22:58 +0100 FeedCreator 1.7.2 http://biowww.net/detail-276.html DNA templates with high GC content and short repeats sometimes are very difficult for PCR amplification. This PCR protocol for overcoming difficult DNA G/C rich regions uses Polyoxyethylenesorbitan monolaurate and Igepal CA-630. (Ziyun Yao, Shaoping Lin, HongM ... biowww http://biowww.net/detail-195.html I am trying to PCR across a tandem repeat region which is highly GC rich (approx 82%). The repeat region is asymmetrical in that one strand contains mostly Cs and the opposite strand mostly Gs. The repeat unit varies in size from 2.5-5Kb with most of ... biowww