http://biowww.net/ Wed, 27 Aug 2008 11:31:16 +0100 FeedCreator 1.7.2 http://biowww.net/detail-1363.html This is the open access version of current protocols on cytokines and cytokines receptors provided by Invitrogen. No registration required to browse protocols. Cytokine and receptor protocols: 1. Measurement of Human and Murine Interleukin 2 and I ... biowww http://biowww.net/detail-1132.html A pdf protocol from Adriana Weinberg, MD. The antigen-specific inducible cytokine production measures the amount of cytokine secreted in the supernatant of antigen-stimulated peripheral blood mononuclear (PBMC) cultures. The assay consists of PBMC stimulati ... biowww http://biowww.net/detail-1313.html Commonly used cytokine assays to detect and quantify cytokine from different samples: 1. Cytokine ELISA assay: ELISA is the most commonly used accurate and sensitive method for detection and quantification of cytokines. 2. Cytokine arrays: Cytokine array ... biowww http://biowww.net/detail-1315.html Protocol written by Protocol prepared by D.K. Mitra, S.C. De Rosa, N. Watanabe from Dr. Stephen C. De Rosa's lab at Stanford University. The basic steps for intracellular staining of cytokine in cell culture: - Harvest and pellet cells. - Stain cells w ... biowww http://biowww.net/detail-493.html Definition of cytokine Mesh term: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cel ... biowww http://biowww.net/detail-1314.html A online animation on cytokine ELISA assay by C.T.L (BD Cellular Technologies Ltd). Basic steps of cytokine ELISA assay: 1. Coat 96 well plate with cytokine capture antibody. 2. Add sample to capture cytokines and non-specific bound are washed off. 3. ... biowww