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        <title>Biowww.net RSS feed</title>
        <description><![CDATA[BioNews feeds from biowww dot net]]></description>
        <link>http://biowww.net/</link>
        <lastBuildDate>Wed, 27 Aug 2008 01:37:41 +0100</lastBuildDate>
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        <item>
            <title>Immunofluorescence Staining &amp; Flow Cytometry of Cell Surface Antigens</title>
            <link>http://biowww.net/detail-406.html</link>
            <description>Protocol from eBioscience, Inc. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>FACScan Startup and Shutdown Procedure</title>
            <link>http://biowww.net/detail-442.html</link>
            <description>From JCSMR FACS Facility. This procedure is for FACScan machine. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>General cell staining protocol for flow cytometry</title>
            <link>http://biowww.net/detail-497.html</link>
            <description>From FACS core facility, University of Connecticut Health Center. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Cytometer FACScan/Calibur/LSR/LSR-2 Operation Training Guide</title>
            <link>http://biowww.net/detail-499.html</link>
            <description>From FACS core facility at University of Connecticut Health Center. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Labelling of Nuclei with BrdU and PI for FACS</title>
            <link>http://biowww.net/detail-818.html</link>
            <description>Protocol of Labelling of Nuclei with BrdU and PI for FACS. (Finkbeiner lab, UCSF). ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>96 well microplate intracellular staining for FACS analysis</title>
            <link>http://biowww.net/detail-1177.html</link>
            <description>Protocol of microplate intracellular staining for FACS analysis from Dr. Cattoretti lab, Columbia University. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Flow cytometry protocols</title>
            <link>http://biowww.net/detail-1232.html</link>
            <description>Flow cytometry protocols from University of Pennsylvania, school of dental medicine.

content:

Direct Surface Immunophenotyping 
PI Staining for Cell Cycle Determination PLUS FITC-Surface Staining 
Three Apoptosis Detection Techniques 
Hoechst/7 ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Cell cycle analysis with PI and BrdU</title>
            <link>http://biowww.net/detail-1233.html</link>
            <description>Cell Cycle Analysis Using Propidium Iodide &amp; Bromodeoxyuridine by W. Roy Overton, Ph.D.

Reagent and detailed procedure included. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Commonly used Cytometry Apoptosis Detection Techniques</title>
            <link>http://biowww.net/detail-1418.html</link>
            <description>Hoechst and 7-AAD

H342 is DNA stain that binds preferentially to A-T base-pairs without the necessary of permeabilization step. However, it is necessary to set up incubation conditions for efficient internalization of the dye. Hoechst 33342 (Bisbenzi ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Common Fluorescent Dyes in Flow Cytometry</title>
            <link>http://biowww.net/detail-408.html</link>
            <description>An introduction on commonly used fluorescent dyes in FACS analysis (from ebioscience inc). ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Compensation in Flow Cytometry Analyses</title>
            <link>http://biowww.net/detail-1127.html</link>
            <description>This method review is written by Mario Roederer. Compensation is probably the least understood process accompanying flow cytometric analyses. Perhaps this is because it is often described with the linear algebra elements needed for its computation, and many of ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Purdue University Cytometry Laboratories Web Site (PUCL)</title>
            <link>http://biowww.net/detail-413.html</link>
            <description>A popular site for cytometry researchers with many valuable resources on cytometry application. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Flow Cytometry on the Web</title>
            <link>http://biowww.net/detail-414.html</link>
            <description>This is the flow cytometry WWW links list located at the Salk Institute CCMI, La Jolla, California. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Collection of cytometry protocols from Janis V. Giorgi Lab</title>
            <link>http://biowww.net/detail-445.html</link>
            <description>List of protocols from the  Janis V. Giorgi cytometry Lab:

Flow Cytometry Glossary 
Method for EtOH fixation of cells for long-term storage and DNA staining 
Minimizing Background Staining 
Blocking FC-Receptor Binding 
Monoclonal Antibody Staining Proc ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>FACS Sensitivity</title>
            <link>http://biowww.net/detail-45.html</link>
            <description>Could anyone tell me the limit of sensitivity of fluorescence activated cell 
 sorting? Is it possible to detect, for example,one labelled cell out of a 
 population of 1 million cells? 
 
 ... ...
 
 Click following links for original posts. 
  ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Optimizing Sorting Experiments</title>
            <link>http://biowww.net/detail-441.html</link>
            <description>This page listed several factors that need to be considered in order to prepare cells properly for a successful FACS. (TSRI Flow Cytometry Protocols) ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Table of Fluorochromes</title>
            <link>http://biowww.net/detail-443.html</link>
            <description>This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted)

From JCSMR FACS lab. ...</description>
            <author>biowww</author>
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