Biowww.net RSS feed http://biowww.net/ Thu, 04 Sep 2008 03:41:30 +0100 FeedCreator 1.7.2 Metabolic Labeling of Cells with 35S http://biowww.net/detail-341.html A protocol from Bjorkman group at the CIT. (pdf format) ... biowww Metabolic labeling of cell proteins with [35S]-met http://biowww.net/detail-1024.html Protocol from Hypertension Research Laboratory, UCSD. The original protocol from An Zhou at Johns Hopkins, 1995 ... biowww Metabolic labeling & Immunoprecipitation http://biowww.net/detail-1025.html A metabolic labeling and IP protocol by Jun Takagi from Springer lab at CBR Institute for Biomedical Research. Cells incorporate 35S-methionine or cysteine during the protein synthesis. Thus it is essential to use Met,Cys-free medium and dialyzed FCS during ... biowww A method for efficient isotopic labeling of recombinant proteins http://biowww.net/detail-1026.html An open access article on A method for efficient isotopic labeling of recombinant proteins. Reference: Journal of Biomolecular NMR, 20: 71¨C75, 2001. Jonathan Marley, Min Lu and Clay Bracken A rapid and efficient approach for preparing isoto ... biowww Protein metabolic labeling http://biowww.net/detail-34.html Original review on protein metabolic labelling for proteomics study. By Robert J. Beynon{ddagger} and Julie M. Pratt, Molecular & Cellular Proteomics 4:857-872, 2005 There is a gradual emergence of the realisation of the advantages of stable isotope labe ... biowww How to show that the proteins are really labeled? http://biowww.net/detail-54.html We have fed a radiolabeled polymer to some bacteria and then did an extraction/fractionation on the culture. One of the fractions is a phenol solution that supposedly contains proteins. What we want to know is whether the bacteria incorporated a ... biowww S-35 and autoradiography http://biowww.net/detail-88.html I am looking for experienced opinion about exposure times for S-35 protein labelling followed by IP and exposing to X-ray film versus Phosphorimager. Which of the ... ... ... biowww