http://biowww.net/ Fri, 05 Sep 2008 13:19:01 +0100 FeedCreator 1.7.2 http://biowww.net/detail-306.html Protocol from Flow Cytometry Core Facility at PrincetonUniversity. PROPIDIUM IODIDE STAINING METHOD ACRIDINE ORANGE STAINING METHOD HOECHST/DAPI STAINING METHOD ... biowww http://biowww.net/detail-307.html Detailed PDF protocol link to LabVelocity.com. Source from BioSource International. In order to study many of the control mechanisms involved in cell cycle regulation, it is critical to synchronize the cells so that the distinct phases of the cycle are bei ... biowww http://biowww.net/detail-479.html Fero lab at Fred Hutchinson cancer center. ... biowww http://biowww.net/detail-769.html This is a protocol using aphidicolin to synchronize whole culture cells in G1/S phase. The amount of aphidicolin (and the time of exposure) may vary depending on the cell line you are working with. (Andrew Murray, Harvard University) ... biowww http://biowww.net/detail-770.html A protocol to synchronize cells in G2/M using nocodazole. Protocol from Science Advisory Board. ... biowww http://biowww.net/detail-771.html This protocol is designed to synchronize HeLa cells at the G1/S border (actually all G1). It is highly effect and gives almost complete synchronization in the culture. Note that this protocol will not work on cell lines with intact p53 apoptotic responses. (Sc ... biowww http://biowww.net/detail-1060.html Review of cell cycle analysis and protocol for DNA content analysis by flow cytometry with detailed FACS analysis illustrations. (Dr.McLeod, Aldaz at UTMD Ander Cancer Center). ... biowww http://biowww.net/detail-772.html A review by Dr.Stephen Coopter on reappraisal of serum starvation, the restriction point, G0, and G1 phase arrest points. Their application on cell cycle analysis. (PDF file) ... biowww http://biowww.net/detail-773.html Method booklet on cell cycle analysis (BioSource international). Content: Introduction ................................................................................2 I. Cell Proliferation ....................................................... ... biowww http://biowww.net/detail-191.html What's the best way to separate cells based on cell cycle phase? I read about something called "counterflow centrifugal elutriation", but have no idea what it is. Can you actually separate cells based on cell cycle phase using FACS or does it just det ... biowww http://biowww.net/detail-192.html I'm investigating cell cycle progression by measuring changes in the amount of cyclin mRNA by RT-PCR. For this I need an internal control gene which is cell-cycle independent, since this is not so for commonly-used internal references such GAPDH or b- ... biowww