http://biowww.net/ Fri, 05 Sep 2008 23:39:48 +0100 FeedCreator 1.7.2 http://biowww.net/detail-736.html This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviously use fewer or more cells, depending on your needs. (Sefton Lab, Salk institute). ... biowww http://biowww.net/detail-625.html The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion of pyruvate to lactate by lactate dehydrogenase (LDH). The latter step requires NADH which is oxidized to NAD+ ... biowww http://biowww.net/detail-647.html Two different types of assays are used to determine myosin's ATPase activity. First, the ATPase activity of myosin in high salt is measured in the presence of EDTA, calcium, or magnesium. Myosin's ATPase is highest in EDTA, and lowest in magnesium. Second, the ... biowww http://biowww.net/detail-648.html The protocol describes how to assay the catalytic activity of purified and reconstituted CFTR. By Ilana Kogan, Mohabir Ramjeesingh and Christine E. Bear, University of Toronto. ... biowww http://biowww.net/detail-649.html PDF protocol from Auble Lab, University of Virginia. ... biowww http://biowww.net/detail-1405.html This protocol describes detailed procedures on determination of the increased enzymatic activity of the caspase-3 class of proteases in apoptotic cells by fluorometric reaction. (R&D systems) ... biowww http://biowww.net/detail-28.html Some annoying probrems !!Please give me advices eg. drug A induced increased level of TGF-beta protein but do not induced increased expression of TGF-beta mRNA How can I approach the translational control ?? It Seems not by nuclear r ... biowww http://biowww.net/detail-32.html Why is the temperature control for growing E. coli under the T7 RNA polymerase/promoter system required? Page 16-6 in "Short Protocols In Molecular Biology 3rd ed. edited by Fred Ausubel et al." explains the procedures for growing E. coli under the T7 ... biowww