Biowww.net RSS feed http://biowww.net/ Tue, 26 Aug 2008 01:14:21 +0100 FeedCreator 1.7.2 A general PCR protocol and procedure note http://biowww.net/detail-314.html General PCR protocol: ________________________________________________________________ 10X reaction buffer (with MgCl2) 5 ul 25mM MgCl2(optional) 3 ul dNTP mix (10mM of each dNTP) ... biowww Amplification and Labeling with Cy dyes http://biowww.net/detail-764.html This is a protocol to synthesize cy dyes incorporated DNA probe using PCR for hybridization. (from YGAC Yale Genome Analysis Center) ... biowww Alkami Quick Guide for PCR http://biowww.net/detail-1139.html A 70 page pdf tech book on PCR. (Alkami Biosystems, Inc.) Content: PREFACE.................................................................................... 4 LAB SUPPLY SELECTION ........................................................ 5 LABO ... biowww Electronic PCR: finding sequence tagged sites STS http://biowww.net/detail-799.html Electronic PCR (e-PCR) is computational procedure that is used to identify sequence tagged sites(STSs), within DNA sequences. e-PCR looks for potential STSs in DNA sequences by searching for subsequences that closely match the PCR primers and have the correct ... biowww