http://biowww.net/ Tue, 26 Aug 2008 09:19:10 +0100 FeedCreator 1.7.2 http://biowww.net/detail-518.html Nice protocol on double staining of cells, tissue sections (from IHC world). The double-immunofluorescence or triple-immunofluorescence technique uses two or three primary antibodies raised from hosts of different species. For example mouse-anti-actin and g ... biowww http://biowww.net/detail-560.html Written by Louise Cramer (actin part)and Arshad Desai (tubulin part), Mitchinson lab at Harvard medical school. Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of ind ... biowww http://biowww.net/detail-271.html This mitochondria staining protocol uses MitoTracker green to stain live cell mitochondria on coverslips. It uses Phalloidin Red to stain the fixed cells for visualization of f-actin. The double staining protocol is from Rob Phillips Group at CIT. ... biowww http://biowww.net/detail-517.html A general Immunofluorescence staining method (IHC world). ... biowww http://biowww.net/detail-519.html Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved (from Giorgio Cattoretti's lab at Columbia University) ... biowww http://biowww.net/detail-568.html Protocol written by Arshad Desai in Mitchinson lab at Harvard medical school. ... biowww http://biowww.net/detail-597.html By Josiah N. Wilcox and Romesh R. Subramanian From WIlcox lab at Emory University. ... biowww http://biowww.net/detail-759.html The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) Protocol from Yale Genome Analysis Center YGAC. ... biowww http://biowww.net/detail-760.html This protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes. Two dishes can be processed simultaneously. The last step is to transfer each sample to a well on a polylysin ... biowww http://biowww.net/detail-1223.html A immunofluorescence protocol for double staining of GFP and BrdU. Doc protocol is from Dr.Gordon Fishell lab at New York University School of Medicine. ... biowww http://biowww.net/detail-1260.html Protocols and buffer recipes on: Autoflourescence Cell Biology Buffers Immunofluorescence Mounting Media Protocol from The MMI Research Core Facility, Ohio State University. ... biowww http://biowww.net/detail-1196.html An excellent primer on fluorescence microscopy from Olympus web site. It covers introduction, advanced techniques, tutorials and troubleshootings. A highly recommended resource for IHC beginners. ... biowww http://biowww.net/detail-456.html Basics and graphic illustration of confocal microscope. (Dr. Eric R. Weeks) ... biowww http://biowww.net/detail-1198.html An introduction to confocal microscopy from Nikon. It features some interactive tutorials. ... biowww http://biowww.net/detail-512.html Troubleshooting and FAQ (BD biosicence): Q I have trouble detecting GFP variant expression in transiently transfected cells. What can I do? Q How can I troubleshoot fluorescent protein expression in yeast? Q How can I troubleshoot no or low signal o ... biowww http://biowww.net/detail-1197.html Fluorescence microscopy optimization tips including image brightness, image resolution. It also includes a troubleshooting guidance table. (Olympus) ... biowww http://biowww.net/detail-1199.html The Bio-Rad fluorescence database has been designed to allow the user to superimpose graphical fluorochrome data from various fluorochromes onto a normalised axis. Both the emission and excitation spectra can be plotted from any fluorochrome in the database ... biowww