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publisher: Wiley, published: 2010-09-28
ASIN: 0470624523
EAN: 9780470624524
sales rank: 1344196
The most complete, up-to-date reference on antigen retrieval and immunohistochemistryAn antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects. Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work: - Summarizes the key problems in the four fields of antigen retrieval
- Discusses the advances of AR techniques and their applications
- Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis
- Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC
- Includes an appendix of related laboratory protocols
Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products. Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor
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by: M.A. Hayat
publisher: Springer, published: 2002-06-30
ASIN: 0306467704
EAN: 9780306467707
sales rank: 2266527
Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
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by: Shan-Rong Shi
publisher: Eaton Publishing Company/Biotechniques Books, published: 2000-11-15
ASIN: 1881299430
EAN: 9781881299431
sales rank: 2234783
(Biotechniques Books) Univ. of Southern California Keck School of Medicine. A textbook and lab guide covering the Antigen Retrieval Technique, its science and its applications. For clinical pathologists, researchers, technicians, and graduate students in immunohistochemistry. DNLM: Antigens--analysis.
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publisher: Springer, published: 2011-07-01
ASIN: 144198061X
EAN: 9781441980618
sales rank: 215608
In a conceptually current, quick-reference, Question & Answer format, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions provides standardization of the immunostaining process for each antibody and for each staining panel. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 5,000 tumors to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Chapters are presented in a unique Question and Answer format. One table/IHC panel is provided to address each question. A concise explanatory note follows each table/panel to avoid diagnostic pitfalls. Website links are provided throughout to update the massive information in this field, providing the most current knowledge and the potential for live expert consultation. All chapters are written by nationally/internationally recognized experts in the related area ensuring authority and excellence. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
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by: Freida L Carson
publisher: American Society for Clinical Pathology, published: 2009-05-08
ASIN: 0891895817
EAN: 9780891895817
sales rank: 184271
An indispensable teaching tool and reference--and a "must" for histotechnologists preparing for the ASCP HTL certifying examination--Histotechnology has been completely updated in the new 3rd edition. New coverage includes chapters on immunohistochemistry and molecular techniques and cytopreparation. You'll also appreciate new features incorporated throughout the book--new images, how-to illustrations for manual techniques, troubleshooting aids, and additional special staining procedures.
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by: Greg T. Hermanson
publisher: Academic Press, published: 2008-05-02
ASIN: 0123705010
EAN: 9780123705013
sales rank: 168546
Bioconjugate Techniques, 2e provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.
*A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab, allowing for fast retrieval of tried and tested techniques for synthesizing bioconjugates.
*Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates.
*Includes more than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates, giving researchers a better understanding of how the commercially available reagents facilitate the crosslinking of peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.
*Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticles Silane coupling agents Dendrimers and dendrons Chemoselective ligation Quantum dots Lanthanide chelates Cyanine dyes Discrete PEG compounds Buckyballs, fullerenes, and carbon nanotubes Mass tags and isotope tags Bioconjugation in the study of protein interactions
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by: Andrew Koob
publisher: FT Press, published: 2009-06-24
ASIN: 0137151713
EAN: 9780137151714
sales rank: 754753
Until recently, neuroscientists thought glial cells did little more than hold your brain together. But in the past few years, they've discovered that glial cells are extraordinarily important. In fact, they may hold the key to understanding intelligence, treating psychiatric disorders and brain injuries and perhaps even curing fatal conditions like Alzheimer's, Parkinson's, and Lou Gehrig's Disease. In The Root of Thought, leading neuroscientist Dr. Andrew Koob reveals what we've learned about these remarkable cells, from their unexpected role in information storage to their function as adult stem cells that can keep your brain growing and adapting longer than scientists ever imagined possible. Ranging from fruit flies to Einstein, Koob reveals the surprising correlation between intelligence and the brain's percentage of glia cells - and why these cells' unique wavelike communications may be especially conducive to the fluid information processing human beings depend upon. You'll learn how crucial glia cells grow and develop... why almost all brain tumors are comprised of glia cells' and the potential implications for treatment... even the apparent role of glia cells in your every thought and dream!
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publisher: Routledge, published: 1990-01-01
ASIN: 0815327455
EAN: 9780815327455
sales rank: 5403268
This is a single volume reference on leucocyte surface antigens, with details on 41 CD antigens newly defined in the late 1990s, and summaries of recent research on all known CD anitgens, collated at the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens. It should be of use to medical libraries and departments of immunology and hematology. New features to this edition include: summary of diagrams for each CD antigens showing at a glance the main features of structure, function, tissue distribution, and the chromosomal location of the gene; separate CD guide in numerical order summarizing key features of each antigen to provide reference on the structure and function of each molecule, with clinical and practical applications; and an antibody appendix (reinstated from the IVth volume), with antibodies listed by: donar, clone name, and blind panel code.
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by: Ralph C. Eagle
publisher: Lippincott Williams & Wilkins, published: 2011-03-02
ASIN: 1608317889
EAN: 9781608317882
sales rank: 167543
Eye Pathology: An Atlas and Text is a basic introduction to eye pathology that can be read and mastered during an ophthalmic pathology rotation. In addition, it will serve as a well-illustrated resource for residents who are studying for the OKAP examinations or Board certification in ophthalmology. Completely written and illustrated by Dr. Eagle, this single-author text is primarily designed for ophthalmology residents and the comprehensive ophthalmologist not looking for a comprehensive text on the subject.
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publisher: Lippincott Williams & Wilkins, published: 2009-08-24
ASIN: 0781779421
EAN: 9780781779425
sales rank: 295213
Completely updated, the Fifth Edition of this standard-setting two-volume reference presents the most advanced diagnostic techniques and the latest information on all currently known disease entities. More than 90 preeminent surgical pathologists offer expert advice on the diagnostic evaluation of every type of specimen from every anatomic site. The Fifth Edition contains over 4,400 full-color photographs. This edition provides detailed coverage of the latest developments in the field, including new molecular and immunohistochemical markers for diagnosis and prognosis of neoplasia, improved classification systems for diagnosis and prognosis, the role of pathology in new diagnostic and therapeutic techniques, and the recognition of new entities or variants of entities. Where appropriate updated World Health Organization (WHO) terminology has been employed for tumor diagnosis. A few of the many specific changes include: - An update on the serrated adenoma and serrated carcinoma pathway
- Virtually new chapters on salivary gland, larynx, blood vessels, heart, non-melanocytic skin tumors, and placenta
- Complete updates of the lymph node and bone marrow chapters incorporating the new WHO terminology
- An expanded discussion of the molecular biology of thyroid neoplasia
All full-color illustrations have been color-balanced to dramatically improve image quality. A companion Website will include the fully searchable text and an image bank.
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