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At the Bench: A Laboratory Navigator, Updated Edition

by: Kathy Barker
publisher: Cold Spring Harbor Laboratory Press, published: 2005-01-01
ASIN: 0879697083
EAN: 9780879697082
sales rank: 82340
At the Bench is the unique and hugely successful handbook for living and working in the laboratory, an essential aid to understanding basic lab techniques and how research groups work at a human level. In this newly revised edition, chapters have been rewritten to accommodate the impact of computer technology and the Internet, not only on the acquisition and analysis of data, but also on its organization and presentation. Alternatives to the use of radiation have been expanded, and figures and illustrations have been redrawn to reflect changes in laboratory equipment and procedures. Related Titles from the Publisher At the Helm: A Laboratory Navigator Lab Ref, Volume 2: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench Lab Dynamics Lab Math

DNA Repair Protocols: Prokaryotic Systems (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617371122
EAN: 9781617371127
Well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.

Molecular Biology Problem Solver: A Laboratory Guide

by: Alan S. Gerstein
publisher: Wiley-Liss, published: 2001-08-24
ASIN: 0471379727
EAN: 9780471379720
sales rank: 417392
Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.
Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
Have you selected the most appropriate research strategy?
Have you identified the issues critical to your successful application of a technique?
Are you familiar with the limitations of a given technique?
When should common procedural rules of thumb not be applied?
What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
Working safely with biological samples and radioactive materials
DNA and RNA purification
PCR
Protein and nucleid acid hybridization
Prokaryotic and eukaryotic expression systems
Properly using and maintaining laboratory equipment

Agrobacterium Protocols: Volume II (Methods in Molecular Biology)

publisher: Humana Press, published: 2006-09-01
ASIN: 1588298434
EAN: 9781588298430
sales rank: 463636
A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Green Fluorescent Protein: Applications & Protocols (Methods in Molecular Biology)

publisher: Springer, published: 2002-06-01
ASIN: 0896039056
EAN: 9780896039056
sales rank: 726301
Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses.

Protein Kinase Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617371416
EAN: 9781617371417
sales rank: 994276
In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and more experienced investigators many novel and time-saving ploys. Emphasis is given to critical technical steps that are often omitted from methods published in the primary literature. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.

Molecular Biology of the Cell, Fourth Edition

by: Bruce Alberts
publisher: Garland Science, published: 2002-03
ASIN: 0815332181
EAN: 9780815332183
sales rank: 67972
Molecular Biology of the Cell is the classic in-dept text reference in cell biology. By extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and create a coherent framework through which non-expert readers may approach the subject. Written in clear and concise language, and beautifully illustrated, the book is enjoyable to read, and it provides a clear sense of the excitement of modern biology. Molecular Biology of the Cell sets forth the current understanding of cell biology (completely updated as of Autumn 2001), and it explores the intriguing implications and possibilities of the great deal that remains unknown. The hallmark features of previous editions continue in the Fourth Edition. The book is designed with a clean and open, single-column layout. The art program maintains a completely consistent format and style, and includes over 1,600 photographs, electron micrographs, and original drawings by the authors. Clear and concise concept headings introduce each section. Every chapter contains extensive references. Most important, every chapter has been subjected to a rigorous, collaborative revision process where, in addition to incorporating comments from expert reviewers, each co-author reads and reviews the other authors' prose. The result is a truly integrated work with a single authorial voice. Features : - Places the latest hot topics sensibly in context - including genomics, protein structure, array technology, stem cells and genetics diseases. - Incorporates and emphasises new genomic data. - All of molecular biology is brought together into one section (chapters 4-7) covering classically defined molecular biology and molecular genetics. - Two chapters deal exclusively with methods and contain information on the latest tools and techniques. - New chapters on "Pathogens, Infection, and Innate Immunity". - Cell Biology Interactive CD-ROM is packaged with every copy of the book. - Contains over 1,600 illustrations, electron micrographs and photographs, of which over 1,000 are originally conceived by the authors.

Short Protocols in Molecular Biology (2 volume set)

publisher: Current Protocols, published: 2002-11-05
ASIN: 0471250929
EAN: 9780471250920
sales rank: 830270
Expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations.

The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research

Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level.

Embryonic Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Methods in Pharmacology and Toxicology)

by: Kursad Turksen
publisher: Humana Press, published: 2001-11-01
ASIN: 0896038815
EAN: 9780896038813
sales rank: 863912
Kursad Turksen and a panel of international experts describe their most productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, the generation of antibodies against very rare antigens, and the identification of and characterization of protein and protein interactions. Embryonic Stem Cells: Methods and Protocols will prove an invaluable resource not only for those interested generally in cell and developmental biology, but also for those actively using, or planning to use ES cells, to study fate choices and specific lineages.

Vascular Biology Protocols (Methods in Molecular Medicine)

publisher: Springer, published: 2012-03-31
ASIN: 1617376507
EAN: 9781617376504
sales rank: 11135196
Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive “manual” in a field that has become as broad and deep as cardiovascular medicine, this volume of “Methods in Molecular Medicine” covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.

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	At the Bench: A Laboratory Navigator, Updated Edition by: Kathy Barker publisher: Cold Spring Harbor Laboratory Press, published: 2005-01-01 ASIN: 0879697083 EAN: 9780879697082 sales rank: 82340 At the Bench is the unique and hugely successful handbook for living and working in the laboratory, an essential aid to understanding basic lab techniques and how research groups work at a human level. In this newly revised edition, chapters have been rewritten to accommodate the impact of computer technology and the Internet, not only on the acquisition and analysis of data, but also on its organization and presentation. Alternatives to the use of radiation have been expanded, and figures and illustrations have been redrawn to reflect changes in laboratory equipment and procedures. Related Titles from the Publisher At the Helm: A Laboratory Navigator Lab Ref, Volume 2: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench Lab Dynamics Lab Math
	DNA Repair Protocols: Prokaryotic Systems (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617371122 EAN: 9781617371127 Well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.
	Molecular Biology Problem Solver: A Laboratory Guide by: Alan S. Gerstein publisher: Wiley-Liss, published: 2001-08-24 ASIN: 0471379727 EAN: 9780471379720 sales rank: 417392 Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. *Molecular Biology Problem Solver: A Laboratory Guide* asks the reader to consider crucial questions, such as: Have you selected the most appropriate research strategy? Have you identified the issues critical to your successful application of a technique? Are you familiar with the limitations of a given technique? When should common procedural rules of thumb not be applied? What strategies could you apply to resolve a problem? A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include: Working safely with biological samples and radioactive materials DNA and RNA purification PCR Protein and nucleid acid hybridization Prokaryotic and eukaryotic expression systems Properly using and maintaining laboratory equipment
	Agrobacterium Protocols: Volume II (Methods in Molecular Biology) publisher: Humana Press, published: 2006-09-01 ASIN: 1588298434 EAN: 9781588298430 sales rank: 463636 A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
	Green Fluorescent Protein: Applications & Protocols (Methods in Molecular Biology) publisher: Springer, published: 2002-06-01 ASIN: 0896039056 EAN: 9780896039056 sales rank: 726301 Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses.
	Protein Kinase Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617371416 EAN: 9781617371417 sales rank: 994276 In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and more experienced investigators many novel and time-saving ploys. Emphasis is given to critical technical steps that are often omitted from methods published in the primary literature. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.
	Molecular Biology of the Cell, Fourth Edition by: Bruce Alberts publisher: Garland Science, published: 2002-03 ASIN: 0815332181 EAN: 9780815332183 sales rank: 67972 Molecular Biology of the Cell is the classic in-dept text reference in cell biology. By extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and create a coherent framework through which non-expert readers may approach the subject. Written in clear and concise language, and beautifully illustrated, the book is enjoyable to read, and it provides a clear sense of the excitement of modern biology. Molecular Biology of the Cell sets forth the current understanding of cell biology (completely updated as of Autumn 2001), and it explores the intriguing implications and possibilities of the great deal that remains unknown. The hallmark features of previous editions continue in the Fourth Edition. The book is designed with a clean and open, single-column layout. The art program maintains a completely consistent format and style, and includes over 1,600 photographs, electron micrographs, and original drawings by the authors. Clear and concise concept headings introduce each section. Every chapter contains extensive references. Most important, every chapter has been subjected to a rigorous, collaborative revision process where, in addition to incorporating comments from expert reviewers, each co-author reads and reviews the other authors' prose. The result is a truly integrated work with a single authorial voice. Features : - Places the latest hot topics sensibly in context - including genomics, protein structure, array technology, stem cells and genetics diseases. - Incorporates and emphasises new genomic data. - All of molecular biology is brought together into one section (chapters 4-7) covering classically defined molecular biology and molecular genetics. - Two chapters deal exclusively with methods and contain information on the latest tools and techniques. - New chapters on "Pathogens, Infection, and Innate Immunity". - Cell Biology Interactive CD-ROM is packaged with every copy of the book. - Contains over 1,600 illustrations, electron micrographs and photographs, of which over 1,000 are originally conceived by the authors.
	Short Protocols in Molecular Biology (2 volume set) publisher: Current Protocols, published: 2002-11-05 ASIN: 0471250929 EAN: 9780471250920 sales rank: 830270 Expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level.
	Embryonic Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Methods in Pharmacology and Toxicology) by: Kursad Turksen publisher: Humana Press, published: 2001-11-01 ASIN: 0896038815 EAN: 9780896038813 sales rank: 863912 Kursad Turksen and a panel of international experts describe their most productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, the generation of antibodies against very rare antigens, and the identification of and characterization of protein and protein interactions. Embryonic Stem Cells: Methods and Protocols will prove an invaluable resource not only for those interested generally in cell and developmental biology, but also for those actively using, or planning to use ES cells, to study fate choices and specific lineages.
	Vascular Biology Protocols (Methods in Molecular Medicine) publisher: Springer, published: 2012-03-31 ASIN: 1617376507 EAN: 9781617376504 sales rank: 11135196 Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive “manual” in a field that has become as broad and deep as cardiovascular medicine, this volume of “Methods in Molecular Medicine” covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.