biotechnology-protocol books

Related books on 'biotechnology-protocol'

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Aqueous Two-Phase Systems: Methods and Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617370673
EAN: 9781617370670
sales rank: 6504363
Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology: Practical Protocols (Springer Lab Manuals)

publisher: Springer, published: 2003-04-28
ASIN: B0019336E4
Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts.
In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.

Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2007-04-05
ASIN: 1588296601
EAN: 9781588296603
sales rank: 1941239
The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume’s five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.

Plant Biotechnology and Genetics: Principles, Techniques and Applications

publisher: Wiley-Interscience, published: 2008-06-02
ASIN: 0470043814
EAN: 9780470043813
sales rank: 777997
Designed to inform and inspire the next generation of plant biotechnologists

Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology.

Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions.

Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online.

This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.

Carbohydrate Biotechnology Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617370746
EAN: 9781617370748
A compilation of modern hands-on methods using microbes and enzymes to produce and modify carbohydrates of potential and actual commercial value. These powerful methods enable both the expert and the beginner to generate polysaccharides, oligosaccharides, and carbohydrate-based surfactants by fermentation using enzymes. Additional techniques make it possible to produce derivatives of sugars, other oligosaccharides, and sugar derivatives using enzyme technology. Carbohydrate Biotechnology Protocols offers synthetic chemists, biochemists, fermentation technologists, and applied enzymologists cutting-edge techniques-many of them hitherto unavailable-that are cleaner and often less costly than available chemical alternatives.

Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 1999-03-15
ASIN: 0896035476
EAN: 9780896035478
sales rank: 3599611
Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.

Downstream Processing of Proteins: Methods and Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617370754
EAN: 9781617370755
sales rank: 5163642
Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology.

Enzymes in Nonaqueous Solvents: Methods and Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617372560
EAN: 9781617372568
sales rank: 4662783
In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design. Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Protocols offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.

Supercritical Fluid Methods and Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617370797
EAN: 9781617370793
Experienced practitioners present detailed accounts of a wide variety of techniques using supercritical fluids. These range from the supercritical fluid extraction methods for numerous compounds to the ninhydrin staining of fingerprints on checks and banknotes-and from the detection of impurities in pharmaceuticals to a wide variety of applications throughout environmental and food science, and across analytical, clinical, and medicinal chemistry. Detailed step-by-step instructions enable users to apply these essential techniques successfully the first time and include modifications that permit their effective adaptation to novel experimental or process conditions. Versatile and comprehensive, Supercritical Fluid Methods and Protocols offers both novice and experienced investigators and laboratory analysts powerful tools that enable successful biological and bioprocess analyses and optimization today.

Pesticide Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2010-12-09
ASIN: 1617375489
EAN: 9781617375484
sales rank: 5405233
A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The readily reproducible methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay methods. The authors have focused on extraction and cleanup procedures, in order to develop and optimize more fullyautomated and miniaturized methods, including solid-phase extraction, solid-phase microextraction, microwave-assisted extraction, and on-line tandem liquid chromatography (LC/LC) trace enrichment, among others. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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	Aqueous Two-Phase Systems: Methods and Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617370673 EAN: 9781617370670 sales rank: 6504363 Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
	Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology: Practical Protocols (Springer Lab Manuals) publisher: Springer, published: 2003-04-28 ASIN: B0019336E4 Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts. In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.
	Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2007-04-05 ASIN: 1588296601 EAN: 9781588296603 sales rank: 1941239 The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume’s five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.
	Plant Biotechnology and Genetics: Principles, Techniques and Applications publisher: Wiley-Interscience, published: 2008-06-02 ASIN: 0470043814 EAN: 9780470043813 sales rank: 777997 Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
	Carbohydrate Biotechnology Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617370746 EAN: 9781617370748 A compilation of modern hands-on methods using microbes and enzymes to produce and modify carbohydrates of potential and actual commercial value. These powerful methods enable both the expert and the beginner to generate polysaccharides, oligosaccharides, and carbohydrate-based surfactants by fermentation using enzymes. Additional techniques make it possible to produce derivatives of sugars, other oligosaccharides, and sugar derivatives using enzyme technology. Carbohydrate Biotechnology Protocols offers synthetic chemists, biochemists, fermentation technologists, and applied enzymologists cutting-edge techniques-many of them hitherto unavailable-that are cleaner and often less costly than available chemical alternatives.
	Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology) publisher: Humana Press, published: 1999-03-15 ASIN: 0896035476 EAN: 9780896035478 sales rank: 3599611 Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.
	Downstream Processing of Proteins: Methods and Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617370754 EAN: 9781617370755 sales rank: 5163642 Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology.
	Enzymes in Nonaqueous Solvents: Methods and Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617372560 EAN: 9781617372568 sales rank: 4662783 In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design. Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Protocols offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.
	Supercritical Fluid Methods and Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617370797 EAN: 9781617370793 Experienced practitioners present detailed accounts of a wide variety of techniques using supercritical fluids. These range from the supercritical fluid extraction methods for numerous compounds to the ninhydrin staining of fingerprints on checks and banknotes-and from the detection of impurities in pharmaceuticals to a wide variety of applications throughout environmental and food science, and across analytical, clinical, and medicinal chemistry. Detailed step-by-step instructions enable users to apply these essential techniques successfully the first time and include modifications that permit their effective adaptation to novel experimental or process conditions. Versatile and comprehensive, Supercritical Fluid Methods and Protocols offers both novice and experienced investigators and laboratory analysts powerful tools that enable successful biological and bioprocess analyses and optimization today.
	Pesticide Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2010-12-09 ASIN: 1617375489 EAN: 9781617375484 sales rank: 5405233 A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The readily reproducible methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay methods. The authors have focused on extraction and cleanup procedures, in order to develop and optimize more fullyautomated and miniaturized methods, including solid-phase extraction, solid-phase microextraction, microwave-assisted extraction, and on-line tandem liquid chromatography (LC/LC) trace enrichment, among others. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.