Related books on 'cell-culture tissue-culture'
Return to life science book listNumber of products: 219331
Page 1 of 21934
|
by: R. Ian Freshney publisher: Wiley-Blackwell, published: 2010-09-17 ASIN: 0470528125 EAN: 9780470528129 sales rank: 144805 This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:
This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.
Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the scientific community, as have many of your other titles. What do you think sets your books apart and makes them so successful compared to other titles in the field? I would say there are three major components: (1) The books are designed as practical guides with detailed single-step instructions which should be sufficient without further recourse to the literature. (2) The preparation and sources of materials are explained in detail. (3) Most of the basic protocols are illustrated with easy-to-follow diagrams. What challenges did you face while putting together Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition? I struggled with the need to update but at the same time preserve basic procedures which have not changed markedly from the previous edition. This, of course, led to the dilemma of deciding what to retain and what to leave out. I also thought long and hard about what specialized protocols to add without compromising the basic technique role of the book. What do you find rewarding about authoring and editing books in the sciences? It forces me to keep up-to-date with developments in cell culture, and in turn helps me to be a better teacher of cell culture. I also get satisfaction from converting what are sometimes complex instructions into one simple straightforward procedure. What inspired you to write your first book? I was invited to prepare a textbook on cell culture for a college course. Previously, I had used John Paul’s book, Cell and Tissue Culture, and knew it needed to be updated. What advice would you offer to scientists writing or editing their first book? - Determine the precise niche that you wish to fill. - Plan the structure carefully beforehand but do not be afraid to deviate, within reason, to meet new demands. - Ensure the text is well illustrated with tables and diagrams. - Make sure the book is properly cross-referenced. - Prepare the index yourself. Even if it is to be professionally indexed you will find terms that the indexer does not. How would you compare the experiences of writing a book and writing a journal article? Both require a clear concise style, but the author has more freedom to express ideas and opinions when writing a book. Another major difference is that a scientific paper must address a specific topic and provide proof to substantiate the conclusions that are drawn, while a book draws on the author’s and other scientists’ experience to provide a more general review and specific guidelines or instructions. Do you find that the role of books in the scientific research community has changed over the years? Are they valued more or less today than they were 10-20 years ago? Rapid changes in technology mean that some books will become outdated without regular updates, which are not always feasible in printed copy. Yet, while there is an increasing tendency to provide instructions and protocols online, many people will still find reassurance from using a textbook with an established reputation. How do you envision the evolution of science writing over the next 5-10 years? Certain journals will still tend to be regarded as more reliable than others, as indicated by their citation indices. However, the number of free public access journals will probably increase, presumably with payment to submit articles. There will be a continuing need for editorial control of journal content due to the proliferation of unedited, non-reviewed material appearing online. What do you feel has been the most significant scientific discovery that has been made during your lifetime? I can really only speak for biology where the elaboration of the genetic code is probably the most significant development. The ability to regulate gene expression and the resultant plasticity of the cell phenotype will create major opportunities in cell culture and its relevance to tissue in vivo. What are you reading right now? This questionnaire! Otherwise, mostly novels and current journals for which I have alerts. |
||
|
publisher: Humana Press, published: 2011-11-05 ASIN: 1617793663 EAN: 9781617793660 sales rank: 419616 Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research. |
||
|
by: R. Ian Freshney publisher: Wiley-Liss, published: 2005-07-29 ASIN: 0471453293 EAN: 9780471453291 sales rank: 474729 The most complete resource on the techniques, equipment,principles, and practices of animal cell culture Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: * An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols * New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering * An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment * Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text * Fully updated references * The clearest, most consistent presentation of step-by-step protocols available * Numerous diagrams, photographs, tables, and charts * Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. |
||
|
by: Michael A. Dirr publisher: Timber Press, published: 2006-01-07 ASIN: 1604690046 EAN: 9781604690040 sales rank: 75753 Compiled by two distinguished professors of horticulture, The Reference Manual of Woody Plant Propagation is a must for professionals and students of horticulture. Over 1,100 species and their propagation requirements by seeds, cuttings, grafting and budding, and tissue culture are discussed in exhaustive detail. Essentially a recipe book for making more trees and shrubs, this reference is a high-level how-to. |
||
|
by: Michael R. Davey publisher: Wiley, published: 2010-05-18 ASIN: 0470686480 EAN: 9780470686485 sales rank: 636190 The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf.
An essential laboratory manual for students and early-career researchers. |
||
|
publisher: Humana Press, published: 2011-04-25 ASIN: 1617790796 EAN: 9781617790799 sales rank: 768602 With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field. |
||
|
by: Lydiane Kyte publisher: Timber Press, published: 1996-10-28 ASIN: 0881923613 EAN: 9780881923612 sales rank: 243288 Acclaimed since its first appearance as the most practical guide to plant tissue culture and widely adopted as a textbook, this standard work is now even better. This expanded edition introduces new developments in biotechnology, such as genetic engineering and cell culture. It provides detailed recipes for propagating plants from more than 30 families. It explains clearly how to set up a propagating laboratory, from a hobbyist's kitchen to an elaborate commercial enterprise. |
||
|
publisher: Humana Press, published: 2009-10-29 ASIN: 1607612917 EAN: 9781607612919 sales rank: 694185 Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study. |
||
|
publisher: Artech House Publishers, published: 2010-05 ASIN: 1596934581 EAN: 9781596934580 sales rank: 619056 This practical book is part of the new "Artech House Methods in Bioengineering" series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. Tissue engineering is an emerging field that involves the combination of materials, cells, and other signals or growth factors to generate new tissue that can be used to repair or replace damaged tissues due to injury or disease. This groundbreaking volume presents the latest methods and protocols for systematically building tissues in 3D configuration outside the body, as well as providing techniques that modulate repair and regeneration processes that occur 'in situ' (in their natural or original place). |
||
|
publisher: Springer, published: 2006-04-11 ASIN: 3540287175 EAN: 9783540287179 sales rank: 117815 Somatic embryogenesis, the initiation of embryos from previously differentiated somatic cells, is a unique process in plants. This volume expands our view of a subject that is important for plant biotechnology, genetics, cell biology, development, and agricultural applications. All chapters present the latest research progress, including functional genomic, genetic, and proteomic approaches. A special focus is placed on the effects of stress, environment, and plant growth regulators on embryogenesis. The role of genes such as Leafy Cotyledons and Baby Boom in defining and maintaining cell competence is discussed. |
