Related books on 'cell-culture'
Return to life science book listNumber of products: 29788
Page 1 of 2979
|
by: R. Ian Freshney publisher: Wiley-Blackwell, published: 2010-09-17 ASIN: 0470528125 EAN: 9780470528129 sales rank: 87471 This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:
This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.
Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the scientific community, as have many of your other titles. What do you think sets your books apart and makes them so successful compared to other titles in the field? I would say there are three major components: (1) The books are designed as practical guides with detailed single-step instructions which should be sufficient without further recourse to the literature. (2) The preparation and sources of materials are explained in detail. (3) Most of the basic protocols are illustrated with easy-to-follow diagrams. What challenges did you face while putting together Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition? I struggled with the need to update but at the same time preserve basic procedures which have not changed markedly from the previous edition. This, of course, led to the dilemma of deciding what to retain and what to leave out. I also thought long and hard about what specialized protocols to add without compromising the basic technique role of the book. What do you find rewarding about authoring and editing books in the sciences? It forces me to keep up-to-date with developments in cell culture, and in turn helps me to be a better teacher of cell culture. I also get satisfaction from converting what are sometimes complex instructions into one simple straightforward procedure. What inspired you to write your first book? I was invited to prepare a textbook on cell culture for a college course. Previously, I had used John Paul’s book, Cell and Tissue Culture, and knew it needed to be updated. What advice would you offer to scientists writing or editing their first book? - Determine the precise niche that you wish to fill. - Plan the structure carefully beforehand but do not be afraid to deviate, within reason, to meet new demands. - Ensure the text is well illustrated with tables and diagrams. - Make sure the book is properly cross-referenced. - Prepare the index yourself. Even if it is to be professionally indexed you will find terms that the indexer does not. How would you compare the experiences of writing a book and writing a journal article? Both require a clear concise style, but the author has more freedom to express ideas and opinions when writing a book. Another major difference is that a scientific paper must address a specific topic and provide proof to substantiate the conclusions that are drawn, while a book draws on the author’s and other scientists’ experience to provide a more general review and specific guidelines or instructions. Do you find that the role of books in the scientific research community has changed over the years? Are they valued more or less today than they were 10-20 years ago? Rapid changes in technology mean that some books will become outdated without regular updates, which are not always feasible in printed copy. Yet, while there is an increasing tendency to provide instructions and protocols online, many people will still find reassurance from using a textbook with an established reputation. How do you envision the evolution of science writing over the next 5-10 years? Certain journals will still tend to be regarded as more reliable than others, as indicated by their citation indices. However, the number of free public access journals will probably increase, presumably with payment to submit articles. There will be a continuing need for editorial control of journal content due to the proliferation of unedited, non-reviewed material appearing online. What do you feel has been the most significant scientific discovery that has been made during your lifetime? I can really only speak for biology where the elaboration of the genetic code is probably the most significant development. The ability to regulate gene expression and the resultant plasticity of the cell phenotype will create major opportunities in cell culture and its relevance to tissue in vivo. What are you reading right now? This questionnaire! Otherwise, mostly novels and current journals for which I have alerts. |
||
|
publisher: Wiley, published: 2011-05-10 ASIN: 0470666587 EAN: 9780470666586 sales rank: 631223 This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable. |
||
|
publisher: Humana Press, published: 2009-10-29 ASIN: 1607612917 EAN: 9781607612919 sales rank: 651489 Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study. |
||
|
by: Michael R. Davey publisher: Wiley, published: 2010-05-18 ASIN: 0470686480 EAN: 9780470686485 sales rank: 596302 The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf.
An essential laboratory manual for students and early-career researchers. |
||
|
publisher: Humana Press, published: 2011-04-25 ASIN: 1617790796 EAN: 9781617790799 sales rank: 724449 With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field. |
||
|
publisher: Oxford University Press, USA, published: 2002-03-21 ASIN: 0199638535 EAN: 9780199638536 sales rank: 945021 The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination. |
||
|
publisher: Humana Press, published: 2011-11-05 ASIN: 1617793663 EAN: 9781617793660 sales rank: 361714 Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research. |
||
|
publisher: Wiley-Blackwell, published: 2011-01-04 ASIN: 0470595450 EAN: 9780470595459 sales rank: 803382 Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features:
The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field. |
||
|
by: Rebecca Skloot publisher: Broadway, published: 2011-03-08 ASIN: 1400052181 EAN: 9781400052189 sales rank: 60 Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells—taken without her knowledge—became one of the most important tools in medicine. The first “immortal” human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. If you could pile all HeLa cells ever grown onto a scale, they’d weigh more than 50 million metric tons—as much as a hundred Empire State Buildings. HeLa cells were vital for developing the polio vaccine; uncovered secrets of cancer, viruses, and the atom bomb’s effects; helped lead to important advances like in vitro fertilization, cloning, and gene mapping; and have been bought and sold by the billions. Yet Henrietta Lacks remains virtually unknown, buried in an unmarked grave. Now Rebecca Skloot takes us on an extraordinary journey, from the “colored” ward of Johns Hopkins Hospital in the 1950s to stark white laboratories with freezers full of HeLa cells; from Henrietta’s small, dying hometown of Clover, Virginia—a land of wooden slave quarters, faith healings, and voodoo—to East Baltimore today, where her children and grandchildren live and struggle with the legacy of her cells. Henrietta’s family did not learn of her “immortality” until more than twenty years after her death, when scientists investigating HeLa began using her husband and children in research without informed consent. And though the cells had launched a multimillion-dollar industry that sells human biological materials, her family never saw any of the profits. As Rebecca Skloot so brilliantly shows, the story of the Lacks family—past and present—is inextricably connected to the dark history of experimentation on African Americans, the birth of bioethics, and the legal battles over whether we control the stuff we are made of. Over the decade it took to uncover this story, Rebecca became enmeshed in the lives of the Lacks family—especially Henrietta’s daughter Deborah, who was devastated to learn about her mother’s cells. She was consumed with questions: Had scientists cloned her mother? Did it hurt her when researchers infected her cells with viruses and shot them into space? What happened to her sister, Elsie, who died in a mental institution at the age of fifteen? And if her mother was so important to medicine, why couldn’t her children afford health insurance? Intimate in feeling, astonishing in scope, and impossible to put down, The Immortal Life of Henrietta Lacks captures the beauty and drama of scientific discovery, as well as its human consequences. |
||
|
publisher: Humana Press, published: 2010-11-02 ASIN: 1607619830 EAN: 9781607619833 sales rank: 408128 Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format. |
