developmental-biology-methods books

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Cell Migration: Developmental Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617375322
EAN: 9781617375323
sales rank: 3895190
A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Plant Developmental Biology: Methods and Protocols (Methods in Molecular Biology)

publisher: Springer, published: 2010-06-11
ASIN: 1607617641
EAN: 9781607617648
sales rank: 1657736
The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation. Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition.

Mitosis: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2009-06-29
ASIN: 160327992X
EAN: 9781603279925
sales rank: 1188624

With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.

Essential Zebrafish Methods: Cell and Developmental Biology (Reliable Lab Solutions)

publisher: Academic Press, published: 2009-09-24
ASIN: 0123745993
EAN: 9780123745996
sales rank: 1390253
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues which make it the model organism for study of vertebrate development. This book will provide overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. The methods and techniques in this volume were chosen by the editors of Methods in Cell Biology, whose goal was to provide fail-safe methods, tips, and "tricks of the trade" to experienced researchers and more junior members in the lab.

* Provides busy researchers a quick reference for time-tested methods and protocols that really work.
* Includes "quick tips and tricks" for each method.
* Provides pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error.

Essential Developmental Biology

by: Jonathan M. W. Slack
publisher: Wiley-Blackwell, published: 2005-10-17
ASIN: 1405122161
EAN: 9781405122160
sales rank: 442722
TO ACCESS THE DEDICATED TEXTBOOK WEBSITE, PLEASE VISIT www.blackwellpublishing.com/slack
Essential Developmental Biology, 2nd Edition, is a concise and well-illustrated treatment of this subject for undergraduates. With an emphasis throughout on the evidence underpinning the main conclusions, this book is suitable as the key text for both introductory and more advanced courses in developmental biology.
Includes new chapters on Evolution & Development, Gut Development, & Growth and Aging.
Contains expanded treatment of mammalian fertilization, the heart and stem cells.
Now features a glossary, notated further reading, and key discovery boxes.
Illustrated with over 250 detailed, full-color drawings.
Accompanied by a dedicated website, featuring animated developmental processes, a photo gallery of selected model organisms, and all art in PowerPoint and jpeg formats (also available to instructors on CD-ROM).
An Instructor manual CD-ROM for this title is available. Please contact our Higher Education team at HigherEducation@wiley.com for more information.

The Biology of the Honey Bee

by: Mark L. Winston
publisher: Harvard University Press, published: 1991-04-01
ASIN: 0674074092
EAN: 9780674074095
sales rank: 182758

From ancient cave paintings of honey bee nests to modern science's richly diversified investigation of honey bee biology and its applications, the human imagination has long been captivated by the mysterious and highly sophisticated behavior of this paragon among insect societies. In the first broad treatment of honey bee biology to appear in decades, Mark Winston provides rare access to the world of this extraordinary insect.
In a bright and engaging style Winston probes the dynamics of the honey bee's social organization. He recreates for us the complex infrastructure of the nest, describes the highly specialized behavior of workers, queens, and drones, and examines in detail the remarkable ability of the honey bee colony to regulate its functions according to events within and outside the nest. Winston integrates into his discussion the results of recent studies, bringing into sharp focus topics of current bee research. These include the exquisite architecture of the nest and its relation to bee physiology; the intricate division of labor and the relevance of a temporal caste structure to efficient functioning of the colony; and, finally, the life-death struggles of swarming, supersedure, and mating that mark the reproductive cycle of the honey bee.
The Biology of the Honey Bee not only reviews the basic aspects of social behavior, ecology, anatomy, physiology, and genetics, it also summarizes major controversies in contemporary honey bee research, such as the importance of kin recognition in the evolution of social behavior and the role of the well-known dance language in honey bee communication. Thorough, well-illustrated, and lucidly written, this book will for many years be a valuable resource for scholars, students, and beekeepers alike.

Molecular Methods in Developmental Biology: Xenopus and Zebrafish (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617371890
EAN: 9781617371899
Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of essential, state-of-the-art techniques in developmental studies, and offers experienced researchers time-saving tips and new techniques from experts who have fine-tuned them for best results.

The Zebrafish: Cellular and Developmental Biology, Part B, Volume 101, Third Edition (Methods in Cell Biology)

publisher: Academic Press, published: 2011-05-13
ASIN: 0123870364
EAN: 9780123870360
sales rank: 1890006
This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Illustrates many techiques in full-color
* Summarizes the Zebrafish Genome Project

Developmental Biology Protocols: Volume I (Methods in Molecular Biology)

publisher: Humana Press, published: 1999-11-19
ASIN: 0896038521
EAN: 9780896038523
sales rank: 3523019
Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.

Developmental Biology Protocols Vol. 2 (Methods in Molecular Biology, 136) (Methods in Molecular Biology)

publisher: Humana Press, published: 2000-03-01
ASIN: 0896035751
EAN: 9780896035751
sales rank: 4034686
Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.

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	Cell Migration: Developmental Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617375322 EAN: 9781617375323 sales rank: 3895190 A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
	Plant Developmental Biology: Methods and Protocols (Methods in Molecular Biology) publisher: Springer, published: 2010-06-11 ASIN: 1607617641 EAN: 9781607617648 sales rank: 1657736 The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation. Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition.
	Mitosis: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2009-06-29 ASIN: 160327992X EAN: 9781603279925 sales rank: 1188624 With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
	Essential Zebrafish Methods: Cell and Developmental Biology (Reliable Lab Solutions) publisher: Academic Press, published: 2009-09-24 ASIN: 0123745993 EAN: 9780123745996 sales rank: 1390253 Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues which make it the model organism for study of vertebrate development. This book will provide overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. The methods and techniques in this volume were chosen by the editors of Methods in Cell Biology, whose goal was to provide fail-safe methods, tips, and "tricks of the trade" to experienced researchers and more junior members in the lab. * Provides busy researchers a quick reference for time-tested methods and protocols that really work. * Includes "quick tips and tricks" for each method. * Provides pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error.
	Essential Developmental Biology by: Jonathan M. W. Slack publisher: Wiley-Blackwell, published: 2005-10-17 ASIN: 1405122161 EAN: 9781405122160 sales rank: 442722 TO ACCESS THE DEDICATED TEXTBOOK WEBSITE, PLEASE VISIT www.blackwellpublishing.com/slack Essential Developmental Biology, 2nd Edition, is a concise and well-illustrated treatment of this subject for undergraduates. With an emphasis throughout on the evidence underpinning the main conclusions, this book is suitable as the key text for both introductory and more advanced courses in developmental biology. Includes new chapters on Evolution & Development, Gut Development, & Growth and Aging. Contains expanded treatment of mammalian fertilization, the heart and stem cells. Now features a glossary, notated further reading, and key discovery boxes. Illustrated with over 250 detailed, full-color drawings. Accompanied by a dedicated website, featuring animated developmental processes, a photo gallery of selected model organisms, and all art in PowerPoint and jpeg formats (also available to instructors on CD-ROM). An Instructor manual CD-ROM for this title is available. Please contact our Higher Education team at HigherEducation@wiley.com for more information.
	The Biology of the Honey Bee by: Mark L. Winston publisher: Harvard University Press, published: 1991-04-01 ASIN: 0674074092 EAN: 9780674074095 sales rank: 182758 From ancient cave paintings of honey bee nests to modern science's richly diversified investigation of honey bee biology and its applications, the human imagination has long been captivated by the mysterious and highly sophisticated behavior of this paragon among insect societies. In the first broad treatment of honey bee biology to appear in decades, Mark Winston provides rare access to the world of this extraordinary insect. In a bright and engaging style Winston probes the dynamics of the honey bee's social organization. He recreates for us the complex infrastructure of the nest, describes the highly specialized behavior of workers, queens, and drones, and examines in detail the remarkable ability of the honey bee colony to regulate its functions according to events within and outside the nest. Winston integrates into his discussion the results of recent studies, bringing into sharp focus topics of current bee research. These include the exquisite architecture of the nest and its relation to bee physiology; the intricate division of labor and the relevance of a temporal caste structure to efficient functioning of the colony; and, finally, the life-death struggles of swarming, supersedure, and mating that mark the reproductive cycle of the honey bee. The Biology of the Honey Bee not only reviews the basic aspects of social behavior, ecology, anatomy, physiology, and genetics, it also summarizes major controversies in contemporary honey bee research, such as the importance of kin recognition in the evolution of social behavior and the role of the well-known dance language in honey bee communication. Thorough, well-illustrated, and lucidly written, this book will for many years be a valuable resource for scholars, students, and beekeepers alike.
	Molecular Methods in Developmental Biology: Xenopus and Zebrafish (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617371890 EAN: 9781617371899 Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of essential, state-of-the-art techniques in developmental studies, and offers experienced researchers time-saving tips and new techniques from experts who have fine-tuned them for best results.
	The Zebrafish: Cellular and Developmental Biology, Part B, Volume 101, Third Edition (Methods in Cell Biology) publisher: Academic Press, published: 2011-05-13 ASIN: 0123870364 EAN: 9780123870360 sales rank: 1890006 This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * Illustrates many techiques in full-color * Summarizes the Zebrafish Genome Project
	Developmental Biology Protocols: Volume I (Methods in Molecular Biology) publisher: Humana Press, published: 1999-11-19 ASIN: 0896038521 EAN: 9780896038523 sales rank: 3523019 Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.
	Developmental Biology Protocols Vol. 2 (Methods in Molecular Biology, 136) (Methods in Molecular Biology) publisher: Humana Press, published: 2000-03-01 ASIN: 0896035751 EAN: 9780896035751 sales rank: 4034686 Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.