Related books on 'flow-cytometry'

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by: Alice Longobardi Givan
publisher: Wiley-Liss, published: 2001-07-23
ASIN: 0471382248
EAN: 9780471382249
sales rank: 293571
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis.

Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:

  • Assumes no prior knowledge of flow cytometry
  • Progresses with an informal, engaging lecture style from simpleto more complex concepts
  • Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis
  • Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields

Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.


by: Tsieh Sun MD
publisher: Lippincott Williams & Wilkins, published: 2011-11-17
ASIN: 1608316165
EAN: 9781608316168
sales rank: 657638
Immunophenotyping is the most powerful tool in the routine diagnosis of hematologic neoplasms. Immunohistochemical technique is used in histology labs for this purpose, while flow cytometry is used in clinical labs. While separately these 2 techniques are very useful in detecting lymphomas and leukemias, the combination of both creates a very powerful and definitive diagnostic tool. The addition of molecular genetics to the book will make it an all-encompassing reference text.

by: Doyen T. Nguyen
publisher: Springer, published: 2007-09-05
ASIN: 1588298558
EAN: 9781588298553
sales rank: 683469
The second edition of this volume reflects the recent advances in the FCM analysis of hematopoietic disorders. The chapters have been revised to incorporate new text and figures. The volume is aimed at hematopathologists, hematologists, pathologists, and laboratory technicians.

by: Wojciech Gorczyca
publisher: Informa Healthcare, published: 2010-02-25
ASIN: 184184702X
EAN: 9781841847023
sales rank: 734920

Flow cytometry is a fast means of reaching a diagnosis in many cases in hematopathology, and is becoming ever more popular as the front line in clinical practice, interesting even those with no basic training in its techniques. However, it is not always the only component necessary for an answer, so correlation with histomorphology is always welcome and often vital.


by: Claudio Ortolani
publisher: Wiley-Blackwell, published: 2011-08-23
ASIN: 144433588X
EAN: 9781444335880
sales rank: 756904
Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference pattern.   There is a detailed description of the main leukocyte antigens, together with a description of their distribution amongst normal and abnormal blood cells. The book also provides a comprehensive description of the phenotype of every neoplastic blood disease recorded in the WHO classification system, including all the instructions needed to recognise and classify even the least common entity.

Designed to be practical, the book is perfect for quick consultation and is divided into two main sections. Section I deals with the direct object of immunophenotyping, and Section II deals with the ultimate target of the analysis. More than 50 antigens are covered and every antigen is dealt with in three main parts: general features, cytometric features and practical hints.

This authoritative and state-of-the-art reference will be invaluable for clinicians directly involved in the diagnosis and analysis of hematological diseases, including hematologists, hematopathologists, oncologists, pathologists and technicians working in diagnostic laboratories.


by: Howard M. Shapiro
publisher: Wiley-Liss, published: 2003-03-14
ASIN: 0471411256
EAN: 9780471411253
sales rank: 761190
From the reviews of the 3rd Edition...

"The standard reference for anyone interested in understanding flow cytometry technology."
American Journal of Clinical Oncology

"...one of the most valuable of its genre and...addressed to a wide audience?written in such an attractive way, being both informative and stimulating."
Trends in Cell Biology

This reference explains the science and discusses the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. Now in its fourth edition, this text has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology today. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers.


by: James V. Watson
publisher: Cambridge University Press, published: 2005-09-15
ASIN: 0521019702
EAN: 9780521019705
sales rank: 844746
This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analyzing dynamic processes from flow cytometry data. These are developed for the analysis of not only individual DNA histograms to obtain the proportion of cells in the cell cycle phases, but also time courses of DNA histograms to yield cell cycle kinetic information; overlapping immunofluorescence distributions with confidence limits for the estimated proportions; enzyme kinetic and membrane transport parameters and a brief introduction to multivariate analysis is given. A distinction is made between data handling, for example gating and counting the numbers of cells within that gate, a process commonly regarded as data analysis but which, in reality, is data handling, and data analysis itself which is the means by which information is extracted.

publisher: Oxford University Press, USA, published: 2005-09-02
ASIN: 0195152344
EAN: 9780195152340
sales rank: 673269
Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes.

Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology.

This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

by: Awtar Krishan Ph.D
publisher: CreateSpace, published: 2010-12-02
ASIN: 1453748601
EAN: 9781453748602
sales rank: 1019280
Detailed descriptions of twenty commonly used protocols for the preparation of cells for flow cytometric analysis. Protocols are written by well-known scientists who taught in international flow cytometry workshops.

publisher: Wiley-VCH, published: 2011-06-07
ASIN: 3527409343
EAN: 9783527409341
sales rank: 1161031
A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions.

This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.

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