gene-targeting books

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Gene Targeting and Embryonic Stem Cells (Advanced Methods)

by: Jim Mcwhir
publisher: Taylor & Francis, published: 2004-03-01
ASIN: 1859963609
EAN: 9781859963609
sales rank: 1493554

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field.

Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.

Lewin's Essential GENES

by: Benjamin Lewin
publisher: Jones & Bartlett Publishers, published: 2009-03-02
ASIN: 0763759155
EAN: 9780763759155
sales rank: 178519
The new edition of Lewin's Essential GENES is the most accessible, student-friendly text of its kind! Completely revised and rewritten, the Second Edition continues to provide students with the latest findings in the field of molecular biology and molecular genetics. An exceptional new pedagogy enhances student learning and helps readers understand and retain key material like never before. New Concept and Reasoning Checks at the end of each chapter section, End of Chapter Questions and Further Readings for each chapter, and several categories of special topics boxes within each chapter expand and reinforce important concepts. The reorganization of topics in this edition allows students to focus more sharply on the key material at hand and improves the natural flow of course material. New end-of-chapter questions reviews major points in the chapter and allow students to test themselves on important course material.

Gene Targeting: A Practical Approach

publisher: Oxford University Press, USA, published: 2000-02-15
ASIN: 019963792X
EAN: 9780199637928
sales rank: 1506058
Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.

Gene Transfer to Animal Cells (Advanced Methods)

by: Richard Twyman
publisher: Taylor & Francis, published: 2005-02-11
ASIN: 1859962041
EAN: 9781859962046
sales rank: 1848403

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles.

Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.

An A to Z of DNA Science: What Scientists Mean When They Talk About Genes and Genomes

by: Jeffre L. Witherly
publisher: Cold Spring Harbor Laboratory Press, published: 2001-10-15
ASIN: 0879696001
EAN: 9780879696009
sales rank: 704973
Medical science constantly demands our attention, as patients or relatives, concerned citizens, voters, investors, or simply curious individuals. But for those without training, the language of science is often hard to follow. The A to Z of DNA Science book series defines and illustrates specialized terms in ways that non-specialists can appreciate and enjoy. This volume focuses on the language of genes, genomes, DNA, biotechnology, and heredity, defining, explaining, and illustrating over 200 terms used in books, broadcasting, websites, and newspaper and magazine articles.

Gene Targeting

by: Manuel A. Vega
publisher: CRC Press, published: 1994-10-25
ASIN: 084938950X
EAN: 9780849389504
sales rank: 5634621
This book examines the revolutionary new technology of gene targeting, from its history to its potential application for the genetic treatment of human diseases. It begins with an introduction to the field and a description of the whole gene targeting spectrum. It continues with discussions in the following areas:
The molecular mechanisms of the homologous recombination reactions lying behind gene targeting technology
The application of gene targeting approaches to the problems of tripanosomatide biology and etiopathology
The concept of gene targeting on plant genes and its impact on plant genetic engineering
The current knowledge on mammalian development and physiology and its relationship to gene targeting technology
The application of gene targeting technology, as well as other genetic techniques, to the production of animal models for human diseases
The enzymatic complexes and components participating in natural bacterial DNA recombination and their potential application to gene targeting technology
The potential application of gene targeting for the genetic treatment of human diseases

Laboratory Protocols for Conditional Gene Targeting

by: Raul M. Torres
publisher: Oxford University Press, USA, published: 1997-10-02
ASIN: 019963677X
EAN: 9780199636778
sales rank: 4134368
Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting--the Institute of Genetics in Cologne--and is written for newcomers and more experienced researchers in the field. Topics include the Cre/loxP recombination system and gene targeting in ES cells; gene targeting vectors; chromosomal translocations; and identification of homologous recombinants.

Vector Targeting for Therapeutic Gene Delivery

by: David T. Curiel
publisher: Wiley-Liss, published: 2002-08-26
ASIN: 0471434795
EAN: 9780471434795
sales rank: 3890358
"...very informative and comprehensive...belongs on the shelf ofevery library and lab providing essential resources for research onvector design or delivery."
-Cancer Biology and Therapy
This comprehensive volume presents the most recent advances in target definitiontechnology and provides a detailed overview on the rational design of targetedvectors for gene therapy. A theoretical framework for advanced vector design is provided that integrates all of the allied sciences relevant to the study of vector targeting. The text discusses the basic underlying science and then leads to discussions of the various viral vectors and methods of definingtargets. Finally, an expert outlook on promising therapeutic applications is offered.

RNA Worlds: From Life's Origins to Diversity in Gene Regulation

by: John F. Atkins
publisher: Cold Spring Harbor Laboratory Press, published: 2010-09-24
ASIN: 0879699469
EAN: 9780879699468
sales rank: 685143
Once thought to be just a messenger that allows genetic information encoded in DNA to direct the formation of proteins, RNA (ribonucleic acid) is now known to be a highly versatile molecule that has multiple roles in cells. It can function as an enzyme, scaffold various subcellular structures, and regulate gene expression through a variety of mechanisms, as well as act as a key component of the protein synthesis and splicing machinery. Perhaps most interestingly, increasing evidence indicates that RNA preceded DNA as the hereditary material and played a crucial role in the early evolution of life on Earth. This volume reviews our understanding of two RNA worlds: the primordial RNA world before DNA, in which RNA was both information store and biocatalyst; and the contemporary RNA world, in which mRNA, tRNA, rRNA, siRNA, miRNA, and a host of other RNAs operate. The early chapters of the book analyze the role of RNA in the first life forms and the appearance of cells. Subsequent chapters examine riboswitches and ribozymes, establishing what the RNA molecule is capable of alone. The book goes on to discuss the evolution of ribosomes and the functions of RNPs, before reviewing the recent work that has revolutionized our understanding of gene regulation by non-coding RNAs, including miRNAs and siRNAs. Also covered are viral RNAs, telomerase RNA, and tools for scientists who work on RNA. The book is thus essential reading for all molecular biologists and biochemists, as well as chemists interested in RNA technology, information storage, or enzyme catalysis.

Gene Targeting

by: John M. Sedivy
publisher: Oxford University Press, USA, published: 1993-09-23
ASIN: 0195099680
EAN: 9780195099683
sales rank: 4540152
Gene targeting offers new approaches to basic research and holds out the promise of eventual correction of genetic diseases. With simple, direct language and extensive illustrations, this advanced textbook offers a comprehensive review of homologous recombination and gene targeting techniques - the basic principles, methods, applicatiuons, and social implications.

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	Gene Targeting and Embryonic Stem Cells (Advanced Methods) by: Jim Mcwhir publisher: Taylor & Francis, published: 2004-03-01 ASIN: 1859963609 EAN: 9781859963609 sales rank: 1493554 Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.
	Lewin's Essential GENES by: Benjamin Lewin publisher: Jones & Bartlett Publishers, published: 2009-03-02 ASIN: 0763759155 EAN: 9780763759155 sales rank: 178519 The new edition of Lewin's Essential GENES is the most accessible, student-friendly text of its kind! Completely revised and rewritten, the Second Edition continues to provide students with the latest findings in the field of molecular biology and molecular genetics. An exceptional new pedagogy enhances student learning and helps readers understand and retain key material like never before. New Concept and Reasoning Checks at the end of each chapter section, End of Chapter Questions and Further Readings for each chapter, and several categories of special topics boxes within each chapter expand and reinforce important concepts. The reorganization of topics in this edition allows students to focus more sharply on the key material at hand and improves the natural flow of course material. New end-of-chapter questions reviews major points in the chapter and allow students to test themselves on important course material.
	Gene Targeting: A Practical Approach publisher: Oxford University Press, USA, published: 2000-02-15 ASIN: 019963792X EAN: 9780199637928 sales rank: 1506058 Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
	Gene Transfer to Animal Cells (Advanced Methods) by: Richard Twyman publisher: Taylor & Francis, published: 2005-02-11 ASIN: 1859962041 EAN: 9781859962046 sales rank: 1848403 Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
	An A to Z of DNA Science: What Scientists Mean When They Talk About Genes and Genomes by: Jeffre L. Witherly publisher: Cold Spring Harbor Laboratory Press, published: 2001-10-15 ASIN: 0879696001 EAN: 9780879696009 sales rank: 704973 Medical science constantly demands our attention, as patients or relatives, concerned citizens, voters, investors, or simply curious individuals. But for those without training, the language of science is often hard to follow. The A to Z of DNA Science book series defines and illustrates specialized terms in ways that non-specialists can appreciate and enjoy. This volume focuses on the language of genes, genomes, DNA, biotechnology, and heredity, defining, explaining, and illustrating over 200 terms used in books, broadcasting, websites, and newspaper and magazine articles.
	Gene Targeting by: Manuel A. Vega publisher: CRC Press, published: 1994-10-25 ASIN: 084938950X EAN: 9780849389504 sales rank: 5634621 This book examines the revolutionary new technology of gene targeting, from its history to its potential application for the genetic treatment of human diseases. It begins with an introduction to the field and a description of the whole gene targeting spectrum. It continues with discussions in the following areas: The molecular mechanisms of the homologous recombination reactions lying behind gene targeting technology The application of gene targeting approaches to the problems of tripanosomatide biology and etiopathology The concept of gene targeting on plant genes and its impact on plant genetic engineering The current knowledge on mammalian development and physiology and its relationship to gene targeting technology The application of gene targeting technology, as well as other genetic techniques, to the production of animal models for human diseases The enzymatic complexes and components participating in natural bacterial DNA recombination and their potential application to gene targeting technology The potential application of gene targeting for the genetic treatment of human diseases
	Laboratory Protocols for Conditional Gene Targeting by: Raul M. Torres publisher: Oxford University Press, USA, published: 1997-10-02 ASIN: 019963677X EAN: 9780199636778 sales rank: 4134368 Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting--the Institute of Genetics in Cologne--and is written for newcomers and more experienced researchers in the field. Topics include the Cre/loxP recombination system and gene targeting in ES cells; gene targeting vectors; chromosomal translocations; and identification of homologous recombinants.
	Vector Targeting for Therapeutic Gene Delivery by: David T. Curiel publisher: Wiley-Liss, published: 2002-08-26 ASIN: 0471434795 EAN: 9780471434795 sales rank: 3890358 "...very informative and comprehensive...belongs on the shelf ofevery library and lab providing essential resources for research onvector design or delivery." -Cancer Biology and Therapy This comprehensive volume presents the most recent advances in target definitiontechnology and provides a detailed overview on the rational design of targetedvectors for gene therapy. A theoretical framework for advanced vector design is provided that integrates all of the allied sciences relevant to the study of vector targeting. The text discusses the basic underlying science and then leads to discussions of the various viral vectors and methods of definingtargets. Finally, an expert outlook on promising therapeutic applications is offered.
	RNA Worlds: From Life's Origins to Diversity in Gene Regulation by: John F. Atkins publisher: Cold Spring Harbor Laboratory Press, published: 2010-09-24 ASIN: 0879699469 EAN: 9780879699468 sales rank: 685143 Once thought to be just a messenger that allows genetic information encoded in DNA to direct the formation of proteins, RNA (ribonucleic acid) is now known to be a highly versatile molecule that has multiple roles in cells. It can function as an enzyme, scaffold various subcellular structures, and regulate gene expression through a variety of mechanisms, as well as act as a key component of the protein synthesis and splicing machinery. Perhaps most interestingly, increasing evidence indicates that RNA preceded DNA as the hereditary material and played a crucial role in the early evolution of life on Earth. This volume reviews our understanding of two RNA worlds: the primordial RNA world before DNA, in which RNA was both information store and biocatalyst; and the contemporary RNA world, in which mRNA, tRNA, rRNA, siRNA, miRNA, and a host of other RNAs operate. The early chapters of the book analyze the role of RNA in the first life forms and the appearance of cells. Subsequent chapters examine riboswitches and ribozymes, establishing what the RNA molecule is capable of alone. The book goes on to discuss the evolution of ribosomes and the functions of RNPs, before reviewing the recent work that has revolutionized our understanding of gene regulation by non-coding RNAs, including miRNAs and siRNAs. Also covered are viral RNAs, telomerase RNA, and tools for scientists who work on RNA. The book is thus essential reading for all molecular biologists and biochemists, as well as chemists interested in RNA technology, information storage, or enzyme catalysis.
	Gene Targeting by: John M. Sedivy publisher: Oxford University Press, USA, published: 1993-09-23 ASIN: 0195099680 EAN: 9780195099683 sales rank: 4540152 Gene targeting offers new approaches to basic research and holds out the promise of eventual correction of genetic diseases. With simple, direct language and extensive illustrations, this advanced textbook offers a comprehensive review of homologous recombination and gene targeting techniques - the basic principles, methods, applicatiuons, and social implications.