Related books on 'immunoassay'
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publisher: Elsevier Science, published: 2005-07-04 ASIN: 0080445268 EAN: 9780080445267 sales rank: 433215 Immunoassays are biochemical tests that rely on antibodies (a special type of protein) to bind to specific molecular structures (antigens). They have become the most commercially successful technology in diagnostics, from home pregnancy testing kits to AIDS testing. Highly illustrated and clearly written, The Immunoassay Handbook provides an excellent guide to this fast-growing industry. Revised and updated, this third edition offers a balanced review of practical information and clinical applications in the field of immunoassay technology. Comprehensive and expertly written, The Immunoassay Handbook will be invaluable for a wide range of users from researchers developing immunoassay systems to developmental and marketing staff who need an excellent overview of how immunoassay principles, systems and applications work. With 68 chapters covering a wide range of topics, and new chapters looking at: surface plasmon resonance in kinetic, concentration and binding site analyses, and the foundations of immunochemistry, this handbook is a must read for all laboratory researchers and academics who use immunoassay as a tool including: pathologists, clinical and veterinary chemists, biochemists, students and researchers in immunology, biochemistry and medicine. |
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publisher: Humana Press, published: 2010-11-05 ASIN: 1617378380 EAN: 9781617378386 sales rank: 1151698 Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community. |
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publisher: Oxford University Press, USA, published: 2000-08-31 ASIN: 0199637105 EAN: 9780199637102 sales rank: 1290300 Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples. |
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by: Q. Zhang publisher: Elsevier, published: 2006-01-18 ASIN: B000PBZYS2 This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: This paper reported the development of a microfludic device for the rapid detection of viable and nonviable microbial cells through dual labeling by fluorescent in situ hybridization (FISH) and quantum dots (QDs)-labeled immunofluorescent assay (IFA). The coin sized device consists of a microchannel and filtering pillars (gap=1-2@mm) and was demonstrated to effectively trap and concentrate microbial cells (i.e. Giardia lamblia). After sample injection, FISH probe solution and QDs-labeled antibody solution were sequentially pumped into the device to accelerate the fluorescent labeling reactions at optimized flow rates (i.e. 1 and 20@mL/min, respectively). After 2min washing for each assay, the whole process could be finished within 30min, with minimum consumption of labeling reagents and superior fluorescent signal intensity. The choice of QDs 525 for IFA resulted in bright and stable fluorescent signal, with minimum interference with the Cy3 signal from FISH detection. |
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Duplexed sandwich immunoassays on a fiber-optic microarray [An article from: Analytica Chimica Acta] by: D.M. Rissin publisher: Elsevier, published: 2006-03-30 ASIN: B000PBZYVY This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: In this paper, we describe a duplexed imaging optical fiber array-based immunoassay for immunoglobulin A (IgA) and lactoferrin. To fabricate the individually addressable array, microspheres were functionalized with highly specific monoclonal antibodies. The microspheres were loaded in microwells etched into the distal face of an imaging optical fiber bundle. Two microsphere-based sandwich immunoassays were developed to simultaneously detect IgA and lactoferrin, two innate immune system proteins found in human saliva. Individual microspheres could be interrogated for the simultaneous measurement of both proteins. The working concentration range for IgA detection was between 700pM and 100nM, while the working concentration range for lactoferrin was between 385pM and 10nM. The cross-reactivity between detection antibodies and their non-specific targets was relatively low in comparison to the signal generated by the specific binding with their targets. These results suggest that the degree of multiplexing on this fiber-optic array platform can be increased beyond a duplex. |
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by: V.N. Morozov publisher: Elsevier, published: 2006-03-30 ASIN: B000PBZYW8 This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: Protein microarrays are becoming a powerful tool in proteome, biochemical, and clinical studies. In addition to the quality of arrayed immobilized probe molecules, sensitivity of the microarray-based assay is highly dependent on the detection technique. Here we suggest four simple techniques for rapid detection of analytes bound to protein microarrays. The techniques employ functionalized magnetic and non-magnetic beads moved to, from, or along the array surface by external forces. In contrast to other labeling techniques actively controlled physical labels: (i) make detection extremely fast to allow microarray reading in seconds; (ii) provide a low background due to active removal of weakly bound beads; and (iii) provide a highly sensitive detection, since one antigen-antibody bond is capable of holding bead immobilized on the array surface. In combination with the electrophoretically assisted active immunoassay we described recently such active reading allows to reduce total indirect immunoassay time to 7-10min while having sensitivity in the femtomolar concentration range. High speed, sensitivity, and specificity make active bead-linked detection an ideal choice in rapid high-throughput screening and in emergency diagnostics. |
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publisher: Academic Press, published: 1996-06-19 ASIN: 0122147308 EAN: 9780122147302 sales rank: 2025345 Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. Key Features * Presents up-to-date information * Provides extensive cross-referencing * Covers theory and practice in full detail * Written by leading authorities |
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publisher: Wiley, published: 1988-06 ASIN: 0471909823 EAN: 9780471909828 sales rank: 2687463 This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology. |
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by: S.S. Deshpande publisher: Springer, published: 1996-01-15 ASIN: 0412056011 EAN: 9780412056017 sales rank: 2486211 This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market. |
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publisher: Academic Press, published: 1983-04-11 ASIN: 0121819922 EAN: 9780121819927 sales rank: 3633821 FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein that appeared in Volume 73 of this series. Methods to enhance the efficiency of producing desired fusion products, to simplify the basic procedures involved, and to assess the specificity and other properties of monoclonal antibodies are discussed. Representative examples illustrate how a battery of monoclonal antibodies with different specificities can be used to study various immunochemical and biochemical problems. Other chapters cover additional procedures that can be used to label antigens and antibodies, separate antigen-antibody complexes, and process data. Some papers classified under one heading or contained in another volume of the series include methods that can be applied in other specific areas. For example, cytotoxicity tests described in Volume 93 may be used to screen supernatant fluids in hybridoma experiments if cytotoxic activity is a desirable property of the monoclonal antibody. |