Related books on 'immunohistochemistry immunocytochemistry'
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by: Richard W. Burry publisher: Springer, published: 2009-12-17 ASIN: 1441913033 EAN: 9781441913036 sales rank: 979326 Description: In biomedical research, because of a dramatic increase in productivity, immunocytochemistry has emerged as a major technique. The proposed book will provide the first practical guide to planning, performing, and evaluating immunocytochemical experiments. In today’s graduate education the emphasis is on doing research and not on formal class work. Graduate students therefore lack the background in many essential techniques necessary to perform research in fields in which they were not trained. As director of a university core microscopy facility which sees students and faculty from dozens of laboratories each year, Dr. Burry has surmised the vast majority of these novice microscope users need considerable help. In an attempt to educate users, Dr. Burry has initiated immunocytochemistry seminars and workshops which serve to train people in this powerful research tool. The proposed book is an outgrowth of these presentations and conversations with, by now, hundreds of people who have asked for help. The philosophy which separates this book from other books in this field is that it is practical, rather than academic. In looking at other important immunocytochemistry titles, the predominant orientation is academic, with the author attempting to comprehensively discuss the topic. For example, one book with sample preparation lists ten fixatives which can be used; however, only two such fixatives are commonly used today. In this particular title, the detailed discussion of old methods might be seen as important in establishing the author as an expert. By contrast, the approach for Burry’s book would be to discuss methods based on what works in animal research laboratories today, and focus only on the most productive methods. An additional distinction with this proposed book is the focus on animal research and not human pathology. There is a certification program for pathology technicians which requires them to learn a set body of material based on processing human tissue for examination by a pathologist. Many of the books on immunocytochemistry aim at this large pathology user base. Due to historical reasons, pathology laboratories process human tissues in a specific way and embed the tissue in paraffin, as has been done for over a century. In the last ten years, the power of immunocytochemistry in clinical diagnosis has become clear and has accordingly been adapted to pathology. However, the extensive processing needed for paraffin sections is not needed if the tissues are from research animals. Processing for animal-based tissues takes about a third of the time and results in higher quality images. The focus of this book is on processing these animal research tissues for immunocytochemistry. Today, there are no technique books which are aimed at this user base. As a subject matter expert in the area of the proposed book, Dr. Burry will make recommendations and offer opinions. Because this field is new and is emerging, there are numerous advantages of specific methods over other, more generalized methods. The purpose of this book is to show a novice how to do immunocytochemistry without engaging in a discussion of possible advanced methods. For the advanced user, there are several good books which discuss the unusual methods, yet for the novice there are currently none. Main Author : Richard W. Burry, The Ohio State University (United States). The Outline of the Book : Each chapter supplies a set of important principals and steps necessary for good immunocytochemistry. The information is distilled down to include only the most important points and does not attempt to cover infrequently used procedures or reagents. At the end of most chapters is a section on trouble-shooting many of the common problems using the Sherlock Holmes method. Each chapter also includes specific protocols which can be used. The goal of each chapter is to present the reader with enough information to successfully design experiments and solve many of the problems one may encounter. Using immunocytochemical protocols without the understanding of their workings is not advised, as the user will need to evaluate his or her results to determine whether the results are reliable. Such evaluation is extremely important for users who need reliable images which will clearly answer important scientific questions. 1. Introduction Definitions (immunocytochemistry and immunohistochemistry) Scope: animal research and not human pathology, paraffin sections, epitope retrieval, or immunohistochemistry Focus: fluorescence and enzyme detection Why do immunocytochemistry? Immunocytochemistry "individual study" rather than "population study" Example of a two-label experiment What is included in these chapters? Overview of the theory Background with enough information to help solve common problems. Advantages and disadvantages of different options Opinions and suggestions 2. Fixation and Sectioning Chemistry of fixation Denaturing vs cross-linking fixatives Application of fixative Perfusion, drop-in, cultures, fresh-frozen Selection of sample section type Sectioning tissue Rapid freezing, cryostat, freezing microtome, vibratome Storage of tissue Protocols 3. Antibodies Introduction Isoforms, structure, reactivity Generation Polyclonal vs monoclonal Antibodies as reagents Antibody specificity and sources Storage and handling 4. Labels for antibodies Fluorescence, enzymes and particulates Fluorescence theory Fluorescent labels - four generations Enzymes theory Selecting enzymes vs. fluorescence Selecting a label- advantages and disadvantages Protocols 5. Methods of applying antibodies Direct method Indirect method Antibody amplification methods ABC TSA Protocols 6. Blocking and Permeability Theory of blocking Theory of detergents Protocols 7. Procedure- Single primary antibody Planning steps Sample, fixation, sectioning Vehicle Antibody dilutions Controls Protocols 8. Multiple primary antibodies - primary antibodies of different species Procedure Controls Protocols 9. Multiple primary antibodies-primary antibodies of same species Block-between Zenon HRP-chromogen development High-titer incubations Controls Protocols 10. Microscopy Wide-field fluorescence microscope Confocal microscope Bright field—enzyme chromogen Choice Problems 11. Images Size, intensity, and pixels Manipulation—what is ethical? Manuscript Figures 11. Planning and Troubleshooting Scheme for discussion-making in planning experiments Case studies with Sherlock Holmes detective work 12. So you want to do electron microscopic ICC? Criteria in decision-making Summary of the two techniques |
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publisher: Springer, published: 2011-07-01 ASIN: 144198061X EAN: 9781441980618 sales rank: 265636 In a conceptually current, quick-reference, Question & Answer format, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions provides standardization of the immunostaining process for each antibody and for each staining panel. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 5,000 tumors to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Chapters are presented in a unique Question and Answer format. One table/IHC panel is provided to address each question. A concise explanatory note follows each table/panel to avoid diagnostic pitfalls. Website links are provided throughout to update the massive information in this field, providing the most current knowledge and the potential for live expert consultation. All chapters are written by nationally/internationally recognized experts in the related area ensuring authority and excellence. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists. |
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by: David J. Dabbs MD publisher: Saunders, published: 2010-01-29 ASIN: 1416057668 EAN: 9781416057666 sales rank: 255159 Diagnostic Immunohistochemistry presents the latest information and most reliable guidance on immunohistological diagnoses in surgical pathology. David J. Dabbs, MD and other leading experts bring you state-of-the-art coverage on genomic and theranostic applications, molecular anatomic pathology, immunocytology, Non-Hodgkin's lymphoma, and more. Additional features such as tables discussing antibody specifications, differential diagnosis boxes, ancillary anatomic molecular diagnostics, and full-color histological images ensure user-friendly coverage that makes key information easy to find and apply. The fully searchable text is also available online at expertconsult.com, along with a downloadable image bank and access to Path Consult. This concise and complete resource is today's indispensable guide to the effective use of immunohistochemical diagnosis.
Accurately interpret immunohistochemical results and provide the clinician with the best therapeutic approaches for the patient Your purchase entitles you to access the web site until the next edition is published, or until the current edition is no longer offered for sale by Elsevier, whichever occurs first. Elsevier reserves the right to offer a suitable replacement product (such as a downloadable or CD-ROM-based electronic version) should access to the web site be discontinued. |
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publisher: Scion Publishing Ltd., published: 2007-01-20 ASIN: 1904842038 EAN: 9781904842033 sales rank: 1279102 Immunohistochemistry has long been a cornerstone technique in biomedical research, but there have been major new developments in recent years, including a growing trend towards automation and high throughput technology. These new techniques, often coupled with some of the established methodology, have seen an expanding range of applications. Immunohistochemistry: Methods Express is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. This book is an essential laboratory manual for all life science researchers who use immunochemistry. It is a book which both experienced researchers and those new to the field will find invaluable. |
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by: M.A. Hayat publisher: Springer, published: 2002-06-30 ASIN: 0306467704 EAN: 9780306467707 sales rank: 2268253 Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians. |
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publisher: Academic Press, published: 2005-08-31 ASIN: 0120884046 EAN: 9780120884049 sales rank: 4882881 Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today. * The only book available that translates molecular genetics into cancer diagnosis * The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations * Methods discussed were either developed or refined by expert contributors in their own laboratories |
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publisher: Academic Press, published: 2005-01-26 ASIN: 0123339421 EAN: 9780123339423 sales rank: 3320284 Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today. This book is the second volume of three planned, individually-sold volumes on this topic. Like Volume 1, this book fully explains the principles and applications of modern techniques used in the field of molecular genetics. It will be of particular interest to pathologists and molecular pathologists conducting both academic and/or clinical research. * The only book available that translates molecular genetics into cancer diagnosis * The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations * Methods discussed were either developed or refined by expert contributors in their own laboratories |
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publisher: Birkhäuser Boston, published: 2000-10-12 ASIN: 0817640657 EAN: 9780817640651 sales rank: 3708185 Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications. |
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by: Igor B. Buchwalow publisher: Springer, published: 2010-02-05 ASIN: 3642046088 EAN: 9783642046087 sales rank: 600729 While many excellent texts and monographs exist which cover various aspects of immunohistochemistry, the lack of a concise comprehensive guide to using the field’s methods was a major motivation for the authors to write this book. The authors carefully selected the methods and easy-to-adopt protocols discussed. In addition to estabished techniques, special attention was paid to current developments in immunohistochemistry such as antigen retrieval, signal amplification, the use of epitope tags, multiple immunolabeling, and diagnostic immunohistochemistry. All procedures examined have been tested by the authors; many are routinely used in daily practice in their institute. Each chapter starts with a short introduction to the respective method’s underlying principles before presenting step-by-step protocols and instructions, including precise recipes for all materials needed. Thus, the book is suitable for a broad audience, ranging from students and technical assistants to experienced researchers. |
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by: J.M. Polak and S. Van Noorden publisher: Garland Science, published: 2003-07-29 ASIN: 1859962084 EAN: 9781859962084 sales rank: 1417236 Now in its third edition, Introduction to Immunocytochemistry is an essential text for newcomers to the field, and will be a valuable reference for more experienced workers. Extensively expanded and updated, this volume covers both background theory and practical applications, including immunostaining methods using fluorescent, enzyme and gold labels. |