Life science and biomedical books

Related books on 'pcr'

	The Correct Way to Fill Out Form PCR-103-u by: Sabrina Chase publisher: Worlds Away Press, published: 2012-01-14 ASIN: B006XL95CY sales rank: 239660 SHORT STORY--Deep in the bowels of the Bureau of Substandards, new hire Boris Niels discovers a vending machine with a very strange coin in the return slot. Can he get a refreshing beverage and still keep his job? Will he ever find his way back to his home dimension? What is it with aliens and paperwork? PLUS: A section from the upcoming science fiction book The Long Way Home.
	PCR Troubleshooting: The Essential Guide by: Michael L. Altshuler publisher: Caister Academic Press, published: 2006-03-01 ASIN: 1904455077 EAN: 9781904455073 sales rank: 645317 This unique polymerase chain reaction (PCR) troubleshooting guide is an essential companion for readers with some experience in PCR. The book discusses the many and varied problems encountered with PCR, together with tips, advice, and procedures to obviate rather than overcome the PCR problems. The advice in PCR Troubleshooting is invaluable.
	A-Z of Quantitative PCR (IUL Biotechnology, No. 5) by: Stephen A. Bustin publisher: International University Line, published: 2004-07 ASIN: 0963681788 EAN: 9780963681782 sales rank: 792367 This book is a comprehensive manual to allow both the novice researcher and the expert to set up and carry out quantitative PCR assays from scratch. However, this book also sets out to explain as many features of qPCR as possible, provide alternative viewpoints, methods, and aims to simulate the researchers into generating, interpreting, and publishing data that are reproducible, reliable, and biologically meaningful
	PCR (THE BASICS (Garland Science)) by: M. J. McPherson publisher: Taylor & Francis, published: 2006-03-30 ASIN: 0415355478 EAN: 9780415355476 sales rank: 927506 A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
	PCR Primer Design (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617377430 EAN: 9781617377433 sales rank: 2434415 At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
	Making PCR: A Story of Biotechnology by: Paul Rabinow publisher: University Of Chicago Press, published: 1997-11-10 ASIN: 0226701476 EAN: 9780226701479 sales rank: 936147 Making PCR is the fascinating, behind-the-scenes account of the invention of one of the most significant biotech discoveries in our time—the polymerase chain reaction. Transforming the practice and potential of molecular biology, PCR extends scientists' ability to identify and manipulate genetic materials and accurately reproduces millions of copies of a given segment in a short period of time. It makes abundant what was once scarce—the genetic material required for experimentation. Making PCR explores the culture of biotechnology as it emerged at Certus Corporation during the 1980s and focuses on its distinctive configuration of scientific, technical, social, economic, political, and legal elements, each of which had its own separate trajectory over the preceding decade. The book contains interviews with the remarkable cast of characters who made PCR, including Kary Mullin, the maverick who received the Nobel prize for "discovering" it, as well as the team of young scientists and the company's business leaders. This book shows how a contingently assembled practice emerged, composed of distinctive subjects, the site where they worked, and the object they invented. "Paul Rabinow paints a . . . picture of the process of discovery in Making PCR: A Story of Biotechnology [and] teases out every possible detail. . . . Makes for an intriguing read that raises many questions about our understanding of the twisting process of discovery itself."—David Bradley, New Scientist "Rabinow's book belongs to a burgeoning genre: ethnographic studies of what scientists actually do in the lab. . . . A bold move."—Daniel Zalewski, Lingua Franca "[Making PCR is] exotic territory, biomedical research, explored. . . . Rabinow describes a dance: the immigration and repatriation of scientists to and from the academic and business worlds."—Nancy Maull, New York Times Book Review
	Real-time PCR (Advanced Methods) publisher: Taylor & Francis, published: 2006-06-13 ASIN: 041537734X EAN: 9780415377348 sales rank: 903825 With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
	PCR Protocols: A Guide to Methods and Applications publisher: Academic Press, published: 1990-01-11 ASIN: 0123721814 EAN: 9780123721815 sales rank: 1110132 The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: * Optimize novel applications * Avoid many cumbersome molecular biological techniques * Set up the laboratory to avoid contamination Key Features * This first-rate guide will help you * Avoid contamination--with specific instructions on setting up your lab * Avoid cumbersome molecular biological techniques * Discover new applications * Simply call our toll-free, 800 number listed below or cut out the coupon, fill in the blanks, and mail it to us with your check, credit-card number, or money order; We'll send you the book and you'll get your answers
	PCR Troubleshooting and Optimization: The Essential Guide publisher: Caister Academic Press, published: 2011-01-01 ASIN: 1904455727 EAN: 9781904455721 sales rank: 1084327 The polymerase chain reaction (PCR) is a fundamental tool in scientific research and clinical testing. Real-time PCR, combining both amplification and detection in one instrument, is a rapid and accurate method for nucleic acid detection and quantification. Although PCR is a very powerful technique, the results achieved are valid only if the appropriate controls have been employed. In addition, proper optimization of PCR conditions is required for the generation of specific, repeatable, reproducible, and sensitive data. This book discusses the strategies for preparing effective controls and standards for PCR, when they should be employed, and how to interpret the information they provide. It highlights the significance of optimization for efficiency, precision, and sensitivity of PCR methodology and provides essential guidance on how to troubleshoot inefficient reactions. Experts in PCR describe design and optimization techniques, discuss the use of appropriate controls, explain the significance of standard curves, and explore the principles and strategies required for effective troubleshooting. The book highlights the importance of sample preparation and quality, primer design, controlling inhibitors, avoiding amplicon and environmental contamination, optimizing reagent quality and concentration, and modifying the thermal cycling protocol for optimal sensitivity and specificity. In addition, specific chapters discuss the history of PCR, the choice of instrumentation, the applications of PCR in metagenomics, high resolution melting analysis, the MIQE guidelines, and PCR at the microliter scale. The strategies, tips and advice contained in this concise volume will enable the scientist to optimize and effectively troubleshoot a wide range of techniques, including PCR, reverse transcriptase PCR, real-time PCR, and quantitative PCR. It will be an essential book for anyone using PCR technology.
	Principles and Technical Aspects of PCR Amplification by: Elizabeth van Pelt-Verkuil publisher: Springer, published: 2010-11-24 ASIN: 9048175798 EAN: 9789048175796 sales rank: 1572875 Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

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