Related books on 'phage-display'
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publisher: Oxford University Press, USA, published: 2004-05-06 ASIN: 019963873X EAN: 9780199638734 sales rank: 988719 Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display - A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike. The book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. An introductory chapter provides an overview of phage biology and phage display, including guidelines for planning a successful phage display experiment. Individual chapters provide protocols for constructing libraries using oligonucleotide-directed mutagenesis or DNA recombination, performing binding selections, and analyzing the binding activities of selected phage clones. Separate chapters then cover common applications, including selection of ligands from peptide libraries, generation of phage antibody libraries and isolation and optimization of antibodies, selection of DNA binding proteins, and expression cloning using cDNA display. Further chapters describe alternative selection strategies, such as selection using immune sera, selection based on enzymatic activity or protein stability, and selection in vivo. Protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples given to customize the procedures to their own protein or selection system of interest. Written by experts in the field, Phage Display - A Practical Approach provides a comprehensive guide to the design and execution of phage display projects, for all those using the technique in basic research and drug discovery. |
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publisher: CRC Press, published: 2005-07-27 ASIN: 0824754662 EAN: 9780824754662 sales rank: 1400312 The first and only guide to showcase the impact of phage display technology on drug discovery, this reference details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. Highlighting the current and future role of phage display in the development of protein therapeutics, this book provides a comprehensive overview that will prove invaluable to anyone researching recombinant antibodies. Daniel E. Levy, editor of the Drug Discovery Series, is the founder of DEL BioPharma, a consulting service for drug discovery programs. He also maintains a blog that explores organic chemistry. |
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publisher: Humana Press, published: 2009-07-16 ASIN: 1603273018 EAN: 9781603273015 sales rank: 1643101 Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory. |
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by: Carlos F. Barbas II publisher: Cold Spring Harbor Laboratory Press, published: 2001-07-01 ASIN: 0879697407 EAN: 9780879697402 sales rank: 1148499 Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application. |
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publisher: Academic Press, published: 1996-10-28 ASIN: 0124023800 EAN: 9780124023802 sales rank: 1891187 Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references |
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by: Santina Carnazza publisher: Nova Science Pub Inc, published: 2010-07 ASIN: 1608769879 EAN: 9781608769872 sales rank: 3843934 Phage display can be considered as a practical realisation of an artificial chemical evolution. As such, it is an useful tool for in vitro protein evolution, aimed to identifying new receptors and natural ligands for 'orphan receptors', drug discovery (peptides might act as enzyme inhibitors and receptor agonists or antagonists, or otherwise modulate the enzyme/receptor's biological effect), 'epitope discovery' (a new approach to disease diagnosis and vaccine development), design of DNA-binding proteins, source of new materials, antibody phage and recombinant phage probes, next-generation nano-electronics, targeted therapy, molecular imaging, gene delivery and phage-display vaccination. All this can be regarded as a kind of synthetic biology in that it involves the reshaping and redirecting of natural molecular systems, phage, typically using the tools of protein and genetic engineering. |
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publisher: CRC Press, published: 2009-07-28 ASIN: 0824754689 EAN: 9780824754686 sales rank: 4269577 A True Insider’s Guide to the Field – Then and Now Until now, there has not been a book that effectively addresses the historical basis of protein discovery. Featuring contributions from a distinguished international panel of experts, Protein Discovery Technologies elucidates the principles, techniques, strategies, and broad range of applications of protein discovery by documenting the often untold stories and personal accounts of the contributors’ past scientific achievements. Incorporates Short, Personal Discovery Vignettes Rather than an exhaustive field analysis, this globally pertinent resource presents in-depth discussions of various methods for protein discovery, including bioinformatics, interaction cloning, protein purification, phage display, non-primate models, and chemical targeting. It also explores biological themes through the examination of cell death, angiogenesis, hemostasis, development, signal transduction, transcriptional control, cell cycle control, neurobiology, and quality control. This readily accessible resource discusses an array of interesting topics, including:
Whereas most books tend to be more specialized, this book contains a broad view of the protein discoveries in many different fields, making Protein Discovery Technologies a valuable reference for today’s researchers, both the new and more seasoned, who are seeking a newfound perspective or a deeper understanding of this exciting field. Daniel E. Levy, editor of the Drug Discovery Series, is the founder of DEL BioPharma, a consulting service for drug discovery programs. He also maintains a blog that explores organic chemistry. |
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by: William Wu publisher: CRC Press, published: 2003-09-29 ASIN: 0849312884 EAN: 9780849312885 sales rank: 4125325 Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development. Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects. Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today. |
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by: William Wu publisher: CRC Press, published: 2011-05-18 ASIN: 1439848300 EAN: 9781439848302 sales rank: 1851646 Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book:
Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research. |
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by: Kevin Roebuck publisher: Tebbo, published: 2011-08-17 ASIN: 174304836X EAN: 9781743048368 A quantum dot display is a type of display technology used in flat panel displays as an electronic visual display. Quantum dots (QD) or semiconductor nanocrystals are a form of light emitting technology and consist of nano-scale crystals that can provide an alternative for applications such as display technology. This display technology differs from cathode ray tubes (CRTs), liquid crystal displays (LCDs), but it is similar to organic light-emitting diode (OLED) displays, in that light is supplied on demand, which enables new, more efficient displays, which is enabling mobile devices with longer battery lives. This book is your ultimate resource for Quantum Dot Displays. Here you will find the most up-to-date information, analysis, background and everything you need to know. In easy to read chapters, with extensive references and links to get you to know all there is to know about Quantum Dot Displays right away, covering: Quantum dot display, Photodetection, Brus equation, Colossal magnetoresistance, Conductance quantum, Coulomb blockade, Coulomb staircase, Electrically detected magnetic resonance, Enhancement or quenching of QD, Q-wire and QW radiations, Giant magnetoresistance, Intersubband polariton, Magnetocapacitance, Molecular beam, Multiple exciton generation, Nanocrystal solar cell, Quantum dot, Quantum dot solar cell, Quantum efficiency, Quantum electrodynamics, Quantum flux parametron, Quantum Hall effect, Quantum heterostructure, Quantum mirage, Quantum point contact, Quantum spin Hall effect, Quantum tunnelling composite, Quantum well, Quantum wire, Quantum-confined Stark effect, Quiteron, Rapid single flux quantum, Spin valve, Trion (physics). This book explains in-depth the real drivers and workings of Quantum Dot Displays. It reduces the risk of your technology, time and resources investment decisions by enabling you to compare your understanding of Quantum Dot Displays with the objectivity of experienced professionals. |