Related books on 'phenotyping'
Return to life science book listNumber of products: 56
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by: Jacqueline N. Crawley PhD publisher: Wiley-Liss, published: 2007-03-23 ASIN: 0471471925 EAN: 9780471471929 sales rank: 369237 Dr. Jacqueline N. Crawley, author of the First and Second Editions of What’s Wrong with My Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice,continues to field calls and e-mails from molecular geneticists who ask: how do I run behavioral assays to find out what’s wrong with my mouse? Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions. The final chapter, "The Next Generation," examines new and emerging technologies. Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers. |
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by: Icon Group International publisher: ICON Group International, Inc., published: 2010-08-17 ASIN: B0063GX55C Webster's bibliographic and event-based timelines are comprehensive in scope, covering virtually all topics, geographic locations and people. They do so from a linguistic point of view, and in the case of this book, the focus is on "Phenotyping," including when used in literature (e.g. all authors that might have Phenotyping in their name). As such, this book represents the largest compilation of timeline events associated with Phenotyping when it is used in proper noun form. Webster's timelines cover bibliographic citations, patented inventions, as well as non-conventional and alternative meanings which capture ambiguities in usage. These furthermore cover all parts of speech (possessive, institutional usage, geographic usage) and contexts, including pop culture, the arts, social sciences (linguistics, history, geography, economics, sociology, political science), business, computer science, literature, law, medicine, psychology, mathematics, chemistry, physics, biology and other physical sciences. This "data dump" results in a comprehensive set of entries for a bibliographic and/or event-based timeline on the proper name Phenotyping, since editorial decisions to include or exclude events is purely a linguistic process. The resulting entries are used under license or with permission, used under "fair use" conditions, used in agreement with the original authors, or are in the public domain. |
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publisher: Oxford University Press, USA, published: 1993-01-14 ASIN: 0199632367 EAN: 9780199632367 sales rank: 10531962 Molecular techniques are now being widely applied in clinical pathology laboratories, yet many people working in these facilities have only limited experience of such procedures. Diagnostic Molecular Pathology: A Practical Approach, Volumes I and II bring together a broad spectrum of protocols relevant to the routine molecular analysis of pathological samples. Volume II demonstrates applications of molecular techniques to clinical samples, including the use of PCR, DNA sequencing, and nucleic acid hybridization for such diverse purposes as pathogen identification and forensic studies. For all the protocols there is expert guidance and detailed instruction on how to ensure accuracy and reliability, which will appeal to people working at all levels in pathology. |
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publisher: Wiley-VCH, published: 2006-08-31 ASIN: 3527310312 EAN: 9783527310319 sales rank: 2636094 This is the first book in the field of mouse genetics to provide comprehensive and standardized methods for the characterization of laboratory mice. The editor is Director of the German Mouse Clinic and member of the Project Committee of the German National Genome Research Network and provides here a brief introduction to the mouse as a model for diseases and functional analysis of genes and proteins. Throughout, he focuses on the characterization of mouse models using the latest phenotyping methods, with the different areas presented in a clearly structured and easily accessible manner. |
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by: Luca Meoli publisher: LAP LAMBERT Academic Publishing, published: 2012-02-07 ASIN: 3847312545 EAN: 9783847312543 |
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publisher: Humana Press, published: 2010-11-19 ASIN: 161737959X EAN: 9781617379598 sales rank: 1229969 During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs. |
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publisher: Humana Press, published: 2011-08-02 ASIN: 1617792039 EAN: 9781617792038 sales rank: 3263781 Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions. In Proprotein Convertases, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway. Providing a timely assessment of impact of activation/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Meticulous and up-to-date, Proprotein Convertases represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well. |
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publisher: Humana Press, published: 2009-09-25 ASIN: 1607612461 EAN: 9781607612469 sales rank: 3344697 As two of the leading causes of death worldwide, heart disease and stroke represent a clear target for genomic research aimed at deciphering the genes and cellular pathways that underlie cardiovascular disease and creating improved therapies. In Cardiovascular Genomics: Methods and Protocols, experts in the field provide methods for cardiovascular phenotyping of rodent models in the first section of the volume and for statistical and bioinformatic integration of phenotype data with genome-wide genotype and expression data in the second section. Understanding these diverse methods will allow an individual laboratory to utilize these genomic methods independently or to better prepare for collaboration with scientists having expertise in other disciplines in order to uncover genes affecting cardiovascular disease. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary equipment, materials, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cardiovascular Genomics: Methods and Protocols will enable researchers to identify causal genes and novel molecular targets that can lead to vital new treatments for cardiovascular disease. |
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publisher: Humana Press, published: 2011-08-31 ASIN: 1617792187 EAN: 9781617792182 sales rank: 4493063 Nuclear magnetic resonance imaging represents a technique that is indispensable in every day biomedical diagnostics. Thanks to the numerous ways to manipulate and detect an NMR signal, it is possible to obtain a variety of information with excellent spatial and temporal resolution. Today’s MRI techniques go far beyond the illustration of pure anatomical structures and include the revealing of processes down to the molecular level. The number of small animal imaging centers relying on MRI as a key method for preclinical research to understand diseases and to test for novel treatments is growing rapidly. In Vivo NMR Imaging: Methods and Protocols is written as an experimental laboratory text to provide a descriptive approach of the various applications of magnetic resonance imaging and its underlying principles. Starting from a compact introduction of basic NMR physics and image encoding techniques suitable for a broad audience in the life sciences, the concept focuses on addressing the many ways of generating contrast in MR images. The authors cover an interdisciplinary range of problems to be addressed by this non-invasive modality, including study protocols for addressing morphological, physiological, functional, and biochemical aspects of various tissues in living organisms. Information about practical aspects of designing experimental studies that follow the special conditions for micro imaging setups are also provided. Written in the successful Methods in Molecular Biology™ series format, In Vivo NMR Imaging: Methods and Protocols aims to be an experimental compendium of modern in vivo MR imaging with special focus on recent developments in molecular imaging and new protocols for imaging metabolism and molecular markers. |
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by: Moriel H Vandsburger publisher: ProQuest, UMI Dissertation Publishing, published: 2011-09-30 ASIN: 124466295X EAN: 9781244662957 Magnetic resonance imaging (MRI) is a powerful and widely used medical imaging modality in clinical practice. When MRI is used to study mice, the roles of individual genes involved in human diseases, ranging from cancers to diabetes and heart disease, can be elucidated using MRI. Typically, when studying the heart with MRI, cardiac MRI (CMR) is used to phenotype genetically altered mice in terms of left ventricular (LV) structure and global function, contractile function, and infarct size and LV remodeling after myocardial infarction (MI). However, development of novel CMR methods could also enable measurement of L-type Ca2+ channel (LTCC) function and myocardial perfusion in the healthy and diseased mouse heart. In this dissertation, the development and applications of a dynamic manganese (Mn)-enhanced CMR method for assessing an index of LTCC function (LTCCI), and an improved arterial spin labeling (ASL) method for measurement of myocardial perfusion are presented. Genetically altered mice have been widely used to study the roles of nitric oxide synthase (NOS) isoforms in the heart. However, the roles of neuronal NOS (nNOS) and endothelial NOS (eNOS) in regulating LTCC and contractile functions remain controversial. In addition, the role of nNOS in modulating myocardial perfusion remains uninvestigated. We used CMR methods to phenotype WT, eNOS -/-, and nNOS-/- mice regarding LV structure, baseline function, beta-adrenergic and muscarinic cholinergic responsiveness, and perfusion reserve. In order to assess in vivo modulation of LTCC function by nNOS and eNOS, we developed a dynamic Mn-enhanced CMR method to quantify LTCCI in the mouse heart under similar physiological stimulation. At baseline, LTCCI was significantly higher in nNOS-/- mice compared to both eNOS-/- and WT mice. In contrast, basal contractile function was similar in all groups. With dobutamine, nNOS-/- mice demonstrated attenuated inotropic and lusitropic reserves and a flat LTCCI response, while both WT and eNOS -/- mice demonstrated positive LTCCI and contractile responses. In response to beta-adrenergic and muscarinic cholinergie stimulation, LTCCI and contractile function decreased from dobutamine levels in all groups. Perfusion and perfusion reserve with either dobutamine or an adenosine receptor agonist are both normal in nNOS-/- mice compared to WT mice. Our results indicate that nNOS, and not eNOS, plays a dominant role in modulating LTCC and contractile functions at baseline, and during beta-adrenergic stimulation. Experimental myocardial infarction (MI) in mice is an important disease model in part due to the ability to study genetic manipulations. MRI has been used to assess cardiac structural and functional changes after MI in mice, but changes in myocardial perfusion after acute MI have not previously been examined. ASL non-invasively measures perfusion, but is sensitive to respiratory motion and heart rate variability, and is difficult to apply after acute MI in mice. To account for these factors, a cardio-respiratory gated (CRG) ASL sequence using a fuzzy C-means algorithm to retrospectively reconstruct images was developed. Using this method, myocardial perfusion was measured in remote and infarcted regions at 1, 7, 14, and 28 days post-MI. Baseline perfusion was 4.9 +/- 0.5 (ml/g·min) and one day post-MI decreased to 0.9 +/- 0.8 (ml/g·min) in infarcted myocardium (P<0.05 vs. baseline) while remaining at 5.2 +/- 0.8 (ml/g·min) in remote myocardium. During the subsequent 28 days, perfusion in the remote zone remained unchanged, while a partial recovery of perfusion in the infarct zone was seen. This technique, when applied to genetically-engineered mice, will allow |