protein-expression books

Related books on 'protein-expression'

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High Throughput Protein Expression and Purification: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617378216
EAN: 9781617378218
sales rank: 2078338
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale

by: Charles Hardin
publisher: Oxford University Press, USA, published: 2001-03-01
ASIN: 0195132947
EAN: 9780195132946
sales rank: 1170071
On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features:
ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques
ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations
ï¿½ Discusses fluorophores, and solvent effects on protein structure
ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature
ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature
ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized
ï¿½ Provides an alphabetized list of common reagents for rapid reference
ï¿½ Offers an extensive index of concepts and terms
ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches

Chromosomal Proteins and Gene Expression (Advances in Experimental Medicine & Biology (Springer))

publisher: Springer, published: 1986-01-01
ASIN: 0306422123
EAN: 9780306422126
sales rank: 763468

Manufacturing of Pharmaceutical Proteins: From Technology to Economy

by: Stefan Behme
publisher: Wiley-VCH, published: 2009-03-17
ASIN: 3527324445
EAN: 9783527324446
sales rank: 698588
This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation.
Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.

Introduction to Protein Science: Architecture, Function, and Genomics

by: Arthur M. Lesk
publisher: Oxford University Press, USA, published: 2010-05-20
ASIN: 0199541302
EAN: 9780199541300
sales rank: 149980
Designed for students in biology, biochemistry, and biomedicine, Introduction to Protein Science, Second Edition, provides a wide-ranging introduction to the contemporary study of proteins in health and disease. The text describes basic principles of protein structure and methods for studying them, illustrates the wide variety of functions that proteins have, and shows how their structures and functions are closely linked.

Introduction to Protein Science, Second Edition, relates the study of proteins to the context of modern high-throughput data streams of genomics and proteomics. It also provides a balanced treatment of the relationship between computational and experimental methods. The text is enhanced by marginal notes, exercises, problems, and "weblems" that develop students' database and computational server skills. An updated and expanded Companion Website offers resources for students and instructors.

New to this edition:

* Coverage of new developments in the field, including high-throughput methods
* A new chapter on enzyme kinetics
* Extended treatments of such topics as protein evolution and the immune system
* New pedagogical features including "key points" and "Proteins in Health and Disease" boxes
* New end-of-chapter exercises, problems, and "weblems"

Protein Expression in Down Syndrome Brain (Springer Medicine)

publisher: Springer, published: 2002-05-17
ASIN: 3211837329
EAN: 9783211837320
sales rank: 4609574
This book presents latest original research work and current opinions on Down syndrome, focussing on the molecular biology of the Down syndrome brain at the protein level, rather than at nucleic acid level, as it is the proteins that do the work, and there is a long and unpredictable way from RNA to protein. The studies used fetal brains with Down syndrome in which no neurodegeneration was found and characterized proteins by two-dimensional gel electrophoresis and mass spectrometry. The book provides new aspects, such as that neuronal density and neurotransmission is unchanged in early life but synaptosomal structures and dendritic spines are already seriously affected at the early second trimester. The presentation of a series of tentative mechanisms for the development of the Down syndrome neurophenotype provides models for present and future thinking on the neurobiology of the Down syndrome brain.

Proteins: Structure and Function

by: David Whitford
publisher: Wiley, published: 2005-05-23
ASIN: 0471498947
EAN: 9780471498940
sales rank: 362370
Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability.
The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins.
An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.

Protein Expression in Mammalian Cells: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2011-10-12
ASIN: 1617793515
EAN: 9781617793516
sales rank: 1435709
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.

Milk Proteins: From Expression to Food (Food Science and Technology)

publisher: Academic Press, published: 2008-10-09
ASIN: 0123740398
EAN: 9780123740397
sales rank: 2912793
In recent years, there has been a great deal of progress in the understanding and management of milk proteins across the production chain. This book takes a uniquely comprehensive look at those developments and presents them in a one-source overview.

By providing a brief overview of each topic area, and then describing the most important recent advances therein, the "field-to-table" approach of this book provides specialists with new and directly relevant information in their own areas, along with information from complementary research fields, allowing them to contextualize their work within the larger pictures. At the same time it provides generalists with a complete overview and offers insights into topics for more in-depth reading.

Covering areas that are receiving attention from people of many fields -- genomics, functional foods -- and including the latest research and developments in milk-protein phenomenon and interactions, this book will be an ideal resource for professionals and students alike.

*A fresh look at recent developments across the entire production chain -- from animal genetics to nutritional and nutrigenomic needs of the customer
*Up-to-date information from internationally-recognised authors from both academic and commercial resources

Heterologous Gene Expression in E.coli: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-12-02
ASIN: 1617379662
EAN: 9781617379666
sales rank: 2275422
Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

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	High Throughput Protein Expression and Purification: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617378216 EAN: 9781617378218 sales rank: 2078338 Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
	Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale by: Charles Hardin publisher: Oxford University Press, USA, published: 2001-03-01 ASIN: 0195132947 EAN: 9780195132946 sales rank: 1170071 On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features: ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations ï¿½ Discusses fluorophores, and solvent effects on protein structure ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized ï¿½ Provides an alphabetized list of common reagents for rapid reference ï¿½ Offers an extensive index of concepts and terms ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches
	Chromosomal Proteins and Gene Expression (Advances in Experimental Medicine & Biology (Springer)) publisher: Springer, published: 1986-01-01 ASIN: 0306422123 EAN: 9780306422126 sales rank: 763468
	Manufacturing of Pharmaceutical Proteins: From Technology to Economy by: Stefan Behme publisher: Wiley-VCH, published: 2009-03-17 ASIN: 3527324445 EAN: 9783527324446 sales rank: 698588 This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation. Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.
	Introduction to Protein Science: Architecture, Function, and Genomics by: Arthur M. Lesk publisher: Oxford University Press, USA, published: 2010-05-20 ASIN: 0199541302 EAN: 9780199541300 sales rank: 149980 Designed for students in biology, biochemistry, and biomedicine, Introduction to Protein Science, Second Edition, provides a wide-ranging introduction to the contemporary study of proteins in health and disease. The text describes basic principles of protein structure and methods for studying them, illustrates the wide variety of functions that proteins have, and shows how their structures and functions are closely linked. Introduction to Protein Science, Second Edition, relates the study of proteins to the context of modern high-throughput data streams of genomics and proteomics. It also provides a balanced treatment of the relationship between computational and experimental methods. The text is enhanced by marginal notes, exercises, problems, and "weblems" that develop students' database and computational server skills. An updated and expanded Companion Website offers resources for students and instructors. New to this edition: * Coverage of new developments in the field, including high-throughput methods * A new chapter on enzyme kinetics * Extended treatments of such topics as protein evolution and the immune system * New pedagogical features including "key points" and "Proteins in Health and Disease" boxes * New end-of-chapter exercises, problems, and "weblems"
	Protein Expression in Down Syndrome Brain (Springer Medicine) publisher: Springer, published: 2002-05-17 ASIN: 3211837329 EAN: 9783211837320 sales rank: 4609574 This book presents latest original research work and current opinions on Down syndrome, focussing on the molecular biology of the Down syndrome brain at the protein level, rather than at nucleic acid level, as it is the proteins that do the work, and there is a long and unpredictable way from RNA to protein. The studies used fetal brains with Down syndrome in which no neurodegeneration was found and characterized proteins by two-dimensional gel electrophoresis and mass spectrometry. The book provides new aspects, such as that neuronal density and neurotransmission is unchanged in early life but synaptosomal structures and dendritic spines are already seriously affected at the early second trimester. The presentation of a series of tentative mechanisms for the development of the Down syndrome neurophenotype provides models for present and future thinking on the neurobiology of the Down syndrome brain.
	Proteins: Structure and Function by: David Whitford publisher: Wiley, published: 2005-05-23 ASIN: 0471498947 EAN: 9780471498940 sales rank: 362370 Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability. The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins. An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.
	Protein Expression in Mammalian Cells: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2011-10-12 ASIN: 1617793515 EAN: 9781617793516 sales rank: 1435709 Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
	Milk Proteins: From Expression to Food (Food Science and Technology) publisher: Academic Press, published: 2008-10-09 ASIN: 0123740398 EAN: 9780123740397 sales rank: 2912793 In recent years, there has been a great deal of progress in the understanding and management of milk proteins across the production chain. This book takes a uniquely comprehensive look at those developments and presents them in a one-source overview. By providing a brief overview of each topic area, and then describing the most important recent advances therein, the "field-to-table" approach of this book provides specialists with new and directly relevant information in their own areas, along with information from complementary research fields, allowing them to contextualize their work within the larger pictures. At the same time it provides generalists with a complete overview and offers insights into topics for more in-depth reading. Covering areas that are receiving attention from people of many fields -- genomics, functional foods -- and including the latest research and developments in milk-protein phenomenon and interactions, this book will be an ideal resource for professionals and students alike. A fresh look at recent developments across the entire production chain -- from animal genetics to nutritional and nutrigenomic needs of the customer Up-to-date information from internationally-recognised authors from both academic and commercial resources
	Heterologous Gene Expression in E.coli: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-12-02 ASIN: 1617379662 EAN: 9781617379666 sales rank: 2275422 Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.