protein-purification books

Related books on 'protein-purification'

Return to life science book list
Number of products: 5200
Page 1 of 520

Guide to Protein Purification, Second Edition (Methods in Enzymology)

publisher: Academic Press, published: 2009-12-03
ASIN: 0123749786
EAN: 9780123749789
sales rank: 393714

The 2eof this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today.

Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology

Assembles chapters on both common and less-common relevant techniques

Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

Protein Purification Techniques: A Practical Approach (Practical Approach Series)

publisher: Oxford University Press, USA, published: 2001-04-05
ASIN: 0199636737
EAN: 9780199636730
sales rank: 615585
Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

Protein Purification: Principles and Practice

by: Robert K. Scopes
publisher: Springer, published: 1993-11-19
ASIN: 0387940723
EAN: 9780387940724
sales rank: 627484

The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand.

Reviews of earlier editions:

"good practical advice that is presented in a pleasantly readable form" --Analytical Biochemistry

"well organized and written clearly" --American Scientist

"should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" --Nature

Manufacturing of Pharmaceutical Proteins: From Technology to Economy

by: Stefan Behme
publisher: Wiley-VCH, published: 2009-03-17
ASIN: 3527324445
EAN: 9783527324446
sales rank: 374028
This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation.
Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.

Basic Methods in Protein Purification and Analysis: A Laboratory Manual

by: Richard J. Simpson
publisher: Cold Spring Harbor Laboratory Press, published: 2008-11-01
ASIN: 0879698675
EAN: 9780879698676
sales rank: 305401
Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts- a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Related Titles from the Publisher Proteins and Proteomics: A Laboratory Manual Protein-Protein Interactions: A Molecular Cloning Manual, 2nd ed. Proteomics: A CSHL Course Manual Molecular Cloning: A Laboratory Manual, 3rd ed.

Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale

by: Charles Hardin
publisher: Oxford University Press, USA, published: 2001-03-01
ASIN: 0195132947
EAN: 9780195132946
sales rank: 988349
On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features:
ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques
ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations
ï¿½ Discusses fluorophores, and solvent effects on protein structure
ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature
ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature
ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized
ï¿½ Provides an alphabetized list of common reagents for rapid reference
ï¿½ Offers an extensive index of concepts and terms
ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches

Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis)

publisher: Wiley, published: 2011-03-22
ASIN: 0471746614
EAN: 9780471746614
sales rank: 1054334
This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
Gel filtration and ion exchange chromatography
Affinity and covalent chromatography
Reversed phase and hydrophobic interaction chromatography
Ligands from combinatorial libraries
Electrophoresis in gels
Immunoelectrophoresis and capillary electrophoresis
Two-dimensional electrophoresis in proteomics
Protein elution and blotting techniques
Large-scale electrophoresis processes

Protein Analysis and Purification: Benchtop Techniques

by: Ian M. Rosenberg
publisher: Birkhäuser Boston, published: 2004-10-21
ASIN: 0817643400
EAN: 9780817643409
sales rank: 1547988
This comprehensive, practical, and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. It will also appeal to experienced researches and graduate students as a benchtop handbook which, without sacrificing scientific concepts, maintains the spirit of experimentation.

Isolation and Purification of Proteins

by: Rajni Hatti-Kaul
publisher: Dekker, published: 2009-02-19
ASIN: B001TMCFCE
sales rank: 846187
No description available

Proteins: Structure and Function

by: David Whitford
publisher: Wiley, published: 2005-05-23
ASIN: 0471498947
EAN: 9780471498940
sales rank: 401597
Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability.
The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins.
An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.

Page: 1 2 > 520

Language for page:
Which server:
Operation:
Product category:
Search parameter:
Search Books:

	Guide to Protein Purification, Second Edition (Methods in Enzymology) publisher: Academic Press, published: 2009-12-03 ASIN: 0123749786 EAN: 9780123749789 sales rank: 393714 The 2eof this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less-common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
	Protein Purification Techniques: A Practical Approach (Practical Approach Series) publisher: Oxford University Press, USA, published: 2001-04-05 ASIN: 0199636737 EAN: 9780199636730 sales rank: 615585 Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
	Protein Purification: Principles and Practice by: Robert K. Scopes publisher: Springer, published: 1993-11-19 ASIN: 0387940723 EAN: 9780387940724 sales rank: 627484 The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" --Analytical Biochemistry "well organized and written clearly" --American Scientist "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" --Nature
	Manufacturing of Pharmaceutical Proteins: From Technology to Economy by: Stefan Behme publisher: Wiley-VCH, published: 2009-03-17 ASIN: 3527324445 EAN: 9783527324446 sales rank: 374028 This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation. Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.
	Basic Methods in Protein Purification and Analysis: A Laboratory Manual by: Richard J. Simpson publisher: Cold Spring Harbor Laboratory Press, published: 2008-11-01 ASIN: 0879698675 EAN: 9780879698676 sales rank: 305401 Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts- a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Related Titles from the Publisher Proteins and Proteomics: A Laboratory Manual Protein-Protein Interactions: A Molecular Cloning Manual, 2nd ed. Proteomics: A CSHL Course Manual Molecular Cloning: A Laboratory Manual, 3rd ed.
	Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale by: Charles Hardin publisher: Oxford University Press, USA, published: 2001-03-01 ASIN: 0195132947 EAN: 9780195132946 sales rank: 988349 On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features: ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations ï¿½ Discusses fluorophores, and solvent effects on protein structure ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized ï¿½ Provides an alphabetized list of common reagents for rapid reference ï¿½ Offers an extensive index of concepts and terms ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches
	Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis) publisher: Wiley, published: 2011-03-22 ASIN: 0471746614 EAN: 9780471746614 sales rank: 1054334 This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography Affinity and covalent chromatography Reversed phase and hydrophobic interaction chromatography Ligands from combinatorial libraries Electrophoresis in gels Immunoelectrophoresis and capillary electrophoresis Two-dimensional electrophoresis in proteomics Protein elution and blotting techniques Large-scale electrophoresis processes
	Protein Analysis and Purification: Benchtop Techniques by: Ian M. Rosenberg publisher: Birkhäuser Boston, published: 2004-10-21 ASIN: 0817643400 EAN: 9780817643409 sales rank: 1547988 This comprehensive, practical, and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. It will also appeal to experienced researches and graduate students as a benchtop handbook which, without sacrificing scientific concepts, maintains the spirit of experimentation.
	Isolation and Purification of Proteins by: Rajni Hatti-Kaul publisher: Dekker, published: 2009-02-19 ASIN: B001TMCFCE sales rank: 846187 No description available
	Proteins: Structure and Function by: David Whitford publisher: Wiley, published: 2005-05-23 ASIN: 0471498947 EAN: 9780471498940 sales rank: 401597 Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability. The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins. An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.