recombinant-protein books

Related books on 'recombinant-protein'

Return to life science book list
Number of products: 7892
Page 1 of 790

Basic Methods in Protein Purification and Analysis: A Laboratory Manual

by: Richard J. Simpson
publisher: Cold Spring Harbor Laboratory Press, published: 2008-11-01
ASIN: 0879698675
EAN: 9780879698676
sales rank: 572627
Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts- a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Related Titles from the Publisher Proteins and Proteomics: A Laboratory Manual Protein-Protein Interactions: A Molecular Cloning Manual, 2nd ed. Proteomics: A CSHL Course Manual Molecular Cloning: A Laboratory Manual, 3rd ed.

High Throughput Protein Expression and Purification: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617378216
EAN: 9781617378218
sales rank: 1934121
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

Biopharmaceuticals: Biochemistry and Biotechnology

by: Gary Walsh
publisher: Wiley-Blackwell, published: 2003-09-19
ASIN: 0470843276
EAN: 9780470843277
sales rank: 1150133
The latest edition of this highly acclaimed textbook, provides a comprehensive and up-to-date overview of the science and medical applications of biopharmaceutical products. Biopharmaceuticals refers to pharmaceutical substances derived from biological sources, and increasingly, it is synonymous with 'newer' pharmaceutical substances derived from genetic engineering or hybridoma technology.

This superbly written review of the important areas of investigation in the field, covers drug production, plus the biochemical and molecular mechanisms of action together with the biotechnology of major biopharmaceutical types on the market or currently under development. There is also additional material reflecting both the technical advances in the area and detailed information on key topics such as the influence of genomics on drug discovery.

Aggregation of Therapeutic Proteins

publisher: Wiley, published: 2010-08-30
ASIN: 0470411961
EAN: 9780470411964
sales rank: 1179044
This book gives pharmaceutical scientists an up-to-date resource on protein aggregation and its consequences, and available methods to control or slow down the aggregation process. While significant progress has been made in the past decade, the current understanding of protein aggregation and its consequences is still immature. Prevention or even moderate inhibition of protein aggregation has been mostly experimental. The knowledge in this book can greatly help pharmaceutical scientists in the development of therapeutic proteins, and also instigate further scientific investigations in this area. This book fills such a need by providing an overview on the causes, consequences, characterization, and control of the aggregation of therapeutic proteins.

Post-translational Modification of Protein Biopharmaceuticals

publisher: Wiley-VCH, published: 2009-06-16
ASIN: 3527320741
EAN: 9783527320745
sales rank: 320365
From the leading author in the field, known around the world for his work and his authoritative publications, the contents of this book have been selected so as to reflect their relative importance for biopharmaceuticals. As a result, around half of the book is devoted to protein glycosylation, while the remainder is made up of other modifications, such as carboxylation, hydroxylation, sulfation, amidation and proteolytic processing. A final section addresses the latest trend of engineering the modification pattern to improve a given biopharmceutical, presenting several recent case studies of successful posttranslational engineering.
This first authoritative overview of the topic is an indispensable guide for drug developers and drug manufacturers with an interest in protein pharmaceuticals.

Recombinant Proteins From Plants: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2008-11-26
ASIN: 1588299783
EAN: 9781588299789
sales rank: 3286294
The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.

Proteins: Structure and Function

by: David Whitford
publisher: Wiley, published: 2005-05-23
ASIN: 0471498947
EAN: 9780471498940
sales rank: 116052
Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability.
The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins.
An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.

Recombinant Proteins from Plants (Methods in Biotechnology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617370215
EAN: 9781617370212
sales rank: 6895248
This authoritative collection of basic and advanced protocols permits biotechnologists to use plants rather than single cell fermentation systems for expressing recombinant proteins. The protocols provide clear step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants, and for the cloning of genes into a number of different plant species. The book also describes promising experimental techniques that will become increasingly important in the future, and includes techniques for the characterization of medically important proteins expressed in plants.

Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis)

publisher: Wiley, published: 2011-03-22
ASIN: 0471746614
EAN: 9780471746614
sales rank: 768842
This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
Gel filtration and ion exchange chromatography
Affinity and covalent chromatography
Reversed phase and hydrophobic interaction chromatography
Ligands from combinatorial libraries
Electrophoresis in gels
Immunoelectrophoresis and capillary electrophoresis
Two-dimensional electrophoresis in proteomics
Protein elution and blotting techniques
Large-scale electrophoresis processes

Principles of Protein X-Ray Crystallography (Springer Advanced Texts in Chemistry)

by: Jan Drenth
publisher: Springer, published: 2006-11-09
ASIN: 0387333347
EAN: 9780387333342
sales rank: 899492

X-ray crystallography is an established method for studying the structure of proteins and other macromolecules. As the importance of proteins grows, researchers in many fields have found that a working knowledge of X-ray diffraction is an indispensable tool. In this new edition of his essential work, the internationally recognized researcher Dr. Jan Drenth offers an up-to-date and technically rigorous introduction to the subject, providing the theoretical background necessary to understand how the structure of proteins is determined at atomic resolution. New material in the 3rd edition includes a section on twinning, an additional chapter on crystal growth and a discussion of single-wavelength anomalous dispersion.

Page: 1 2 > 790

Language for page:
Which server:
Operation:
Product category:
Search parameter:
Search Books:

	Basic Methods in Protein Purification and Analysis: A Laboratory Manual by: Richard J. Simpson publisher: Cold Spring Harbor Laboratory Press, published: 2008-11-01 ASIN: 0879698675 EAN: 9780879698676 sales rank: 572627 Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts- a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Related Titles from the Publisher Proteins and Proteomics: A Laboratory Manual Protein-Protein Interactions: A Molecular Cloning Manual, 2nd ed. Proteomics: A CSHL Course Manual Molecular Cloning: A Laboratory Manual, 3rd ed.
	High Throughput Protein Expression and Purification: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617378216 EAN: 9781617378218 sales rank: 1934121 Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
	Biopharmaceuticals: Biochemistry and Biotechnology by: Gary Walsh publisher: Wiley-Blackwell, published: 2003-09-19 ASIN: 0470843276 EAN: 9780470843277 sales rank: 1150133 The latest edition of this highly acclaimed textbook, provides a comprehensive and up-to-date overview of the science and medical applications of biopharmaceutical products. Biopharmaceuticals refers to pharmaceutical substances derived from biological sources, and increasingly, it is synonymous with 'newer' pharmaceutical substances derived from genetic engineering or hybridoma technology. This superbly written review of the important areas of investigation in the field, covers drug production, plus the biochemical and molecular mechanisms of action together with the biotechnology of major biopharmaceutical types on the market or currently under development. There is also additional material reflecting both the technical advances in the area and detailed information on key topics such as the influence of genomics on drug discovery.
	Aggregation of Therapeutic Proteins publisher: Wiley, published: 2010-08-30 ASIN: 0470411961 EAN: 9780470411964 sales rank: 1179044 This book gives pharmaceutical scientists an up-to-date resource on protein aggregation and its consequences, and available methods to control or slow down the aggregation process. While significant progress has been made in the past decade, the current understanding of protein aggregation and its consequences is still immature. Prevention or even moderate inhibition of protein aggregation has been mostly experimental. The knowledge in this book can greatly help pharmaceutical scientists in the development of therapeutic proteins, and also instigate further scientific investigations in this area. This book fills such a need by providing an overview on the causes, consequences, characterization, and control of the aggregation of therapeutic proteins.
	Post-translational Modification of Protein Biopharmaceuticals publisher: Wiley-VCH, published: 2009-06-16 ASIN: 3527320741 EAN: 9783527320745 sales rank: 320365 From the leading author in the field, known around the world for his work and his authoritative publications, the contents of this book have been selected so as to reflect their relative importance for biopharmaceuticals. As a result, around half of the book is devoted to protein glycosylation, while the remainder is made up of other modifications, such as carboxylation, hydroxylation, sulfation, amidation and proteolytic processing. A final section addresses the latest trend of engineering the modification pattern to improve a given biopharmceutical, presenting several recent case studies of successful posttranslational engineering. This first authoritative overview of the topic is an indispensable guide for drug developers and drug manufacturers with an interest in protein pharmaceuticals.
	Recombinant Proteins From Plants: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2008-11-26 ASIN: 1588299783 EAN: 9781588299789 sales rank: 3286294 The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.
	Proteins: Structure and Function by: David Whitford publisher: Wiley, published: 2005-05-23 ASIN: 0471498947 EAN: 9780471498940 sales rank: 116052 Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability. The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins. An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.
	Recombinant Proteins from Plants (Methods in Biotechnology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617370215 EAN: 9781617370212 sales rank: 6895248 This authoritative collection of basic and advanced protocols permits biotechnologists to use plants rather than single cell fermentation systems for expressing recombinant proteins. The protocols provide clear step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants, and for the cloning of genes into a number of different plant species. The book also describes promising experimental techniques that will become increasingly important in the future, and includes techniques for the characterization of medically important proteins expressed in plants.
	Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis) publisher: Wiley, published: 2011-03-22 ASIN: 0471746614 EAN: 9780471746614 sales rank: 768842 This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography Affinity and covalent chromatography Reversed phase and hydrophobic interaction chromatography Ligands from combinatorial libraries Electrophoresis in gels Immunoelectrophoresis and capillary electrophoresis Two-dimensional electrophoresis in proteomics Protein elution and blotting techniques Large-scale electrophoresis processes
	Principles of Protein X-Ray Crystallography (Springer Advanced Texts in Chemistry) by: Jan Drenth publisher: Springer, published: 2006-11-09 ASIN: 0387333347 EAN: 9780387333342 sales rank: 899492 X-ray crystallography is an established method for studying the structure of proteins and other macromolecules. As the importance of proteins grows, researchers in many fields have found that a working knowledge of X-ray diffraction is an indispensable tool. In this new edition of his essential work, the internationally recognized researcher Dr. Jan Drenth offers an up-to-date and technically rigorous introduction to the subject, providing the theoretical background necessary to understand how the structure of proteins is determined at atomic resolution. New material in the 3rd edition includes a section on twinning, an additional chapter on crystal growth and a discussion of single-wavelength anomalous dispersion.