Related books on 'rna-methods'
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by: Donald C. Rio publisher: Cold Spring Harbor Laboratory Press, published: 2010-11-29 ASIN: 0879698918 EAN: 9780879698911 sales rank: 379964 So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating but the new book RNA: A Laboratory Manual provides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in three of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences. |
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publisher: Humana Press, published: 2011-05-26 ASIN: 1617379085 EAN: 9781617379086 sales rank: 2675892 RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA’s unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies. |
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publisher: Springer, published: 2012-02-29 ASIN: 3642225160 EAN: 9783642225161 Recent progress in high-throughput technologies and genome wide transcriptome studies have lead to a significant scientific milestone of discovering non-coding RNAs (ncRNAs) which spans through a major portion of the genome. These RNAs most often act as riboregulators, and actively participate in the regulation of important cellular functions at the transcriptional and/or post-transcriptional levels rather than simply being an intermediated messenger between DNA and proteins. As the appreciation for the importance of ncRNAs continues to emerge, it is also increasingly clear that these play critical roles in gene regulatory processes during development and differentiation. Further, regulatory RNAs are useful biomarkers for diagnosis of diseases. Hence these RNA regulators are essential to the development of therapeutics. This book on “Regulatory RNAs” offers a comprehensive view on our current understanding of these regulatory RNAs viz. siRNA, miRNA, piRNA, snoRNA, long non-coding RNA, small RNA etc. It addresses both the biogenesis and mechanism of action of regulatory RNAs with a primary focus on their annotation, experimental methodologies (microarray, next-gen sequencing etc.) for their discovery, computational tools for their prediction, and above all, applications of these revolutionary regulatory molecules in understanding biological systems and diseases, including therapeutics. This comprehensive volume is intended for readers with research or teaching interests in ncRNA biology and will provide a major information resource on current research in the fast-moving fields of RNA and gene expression regulation. Cutting-edge and concise, “Regulatory RNAs: Basics, Methods and Applications” promises to support vital research in the field of regulatory RNAs, ever-continuing to grow rapidly and gain increasing importance in basic and translational biology. |
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by: John Kiernan publisher: Cold Spring Harbor Laboratory Press, published: 2008-03-01 ASIN: 1904842429 EAN: 9781904842422 sales rank: 185954 The chemical and physical principles of fixation, staining, and histochemistry in one volume! Now in its fourth edition, Histological and Histochemical Methods has been expanded and updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, descriptive terms. This book is recommended for lecturers and students in biological and biomedical disciplines using histological and histochemical techniques. Postgraduate researchers will find this to be the ideal laboratory companion, as will medical laboratory technologists preparing for their professional examinations. |
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by: William Wu publisher: CRC Press, published: 2011-05-18 ASIN: 1439848300 EAN: 9781439848302 sales rank: 1849941 Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book:
Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research. |
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publisher: Academic Press, published: 2008-12-29 ASIN: 0123743788 EAN: 9780123743787 sales rank: 1316674 Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. The control of biological processes, such as cellular growth and differentiation, is dependent on how the genetic material within a cell is expressed. The cellular physiology of mRNA-including mRNA processing, transport, localization, and turnover-is central to the process of gene expression. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway. Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Offers step-by-step lab instructions including necessary equipment and reagents |
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publisher: Humana Press, published: 2010-12-10 ASIN: 1588299139 EAN: 9781588299130 sales rank: 2905765 Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of “gene X” and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology™ series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it. |
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publisher: CRC Press, published: 2011-12-12 ASIN: 1420069381 EAN: 9781420069389 sales rank: 868864 Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research. |
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by: Ronald B. Corley publisher: Springer, published: 2010-10-15 ASIN: 1441935606 EAN: 9781441935601 sales rank: 1037771 Thousands of methods have been developed in the various biomedical disciplines, and those covered in this book represent the basic, essential and most widely used methods in several different disciplines. |
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publisher: Academic Press, published: 1989-12-12 ASIN: 0121820815 EAN: 9780121820817 sales rank: 3477384 Many methods have been developed or adapted for the study of RNA processing, which plays a key role in the expression of genetic information. Volumes 180 and 181 of Methods in Enzymology, RNA Processing, Parts A and B, represent the first comprehensive collation of protocols used for the preparation of substrates, for the preparation of extracts and enzymes, and for the analysis of products in RNA processing. At least half of the chapters present techniques that are directly applicable to other areas of nucleic acid biochemistry and cell biology. |