Related books on 'single-nucleotide-polymorphisms'
Return to life science book listNumber of products: 7676
Page 1 of 768
|
by: S. Nishizawa publisher: Elsevier, published: 2006-01-18 ASIN: B000PBZYQE This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: In combination with abasic site (AP site)-containing DNAs, potential use of a biotic fluorescence compound, Vitamin B"2 (riboflavin), is demonstrated for the fluorescence detection of the thymine (T)-related single-nucleotide polymorphisms. Our method is based on construction of the AP site in DNA duplexes, which allows small ligands to bind to target nucleotides accompanied by fluorescence signaling: an AP site-containing probe DNA is hybridized with a target DNA so as to place the AP site toward a target nucleobase, by which hydrophobic microenvironments are provided for ligands to recognize target nucleotides through stacking and hydrogen-bonding interactions. In 10mM sodium cacodylate buffer solutions (pH 7.0) containing 100mM NaCl and 1.0mM EDTA, Vitamin B"2 is found to selectively bind to T (K"1"1=1.8x10^6M^-^1 at 5^oC) over other nucleobases, and this is accompanied by significant quenching of its fluorescence. While the sensing functions depend on the flanking sequences to the AP site, Vitamin B"2 is applicable to the detection of T/C (cytosine), T/G (guanine) and T/A (adenine) mutation sequences of the CYP2A6 gene, where the flanking nucleobases are guanines in both positions (-GXG-, X=AP site). |
|
by: Zhanjiang (John) Liu publisher: Wiley-Blackwell, published: 2011-03-01 ASIN: 0813806372 EAN: 9780813806372 sales rank: 640296 Recent developments in DNA marker technologies, in particular the emergence of Single Nucleotide Polymorphism (SNP) discovery, have rendered some of the traditional methods of genetic research outdated. Next Generation Sequencing and Whole Genome Selection in Aquaculture comprehensively covers the current state of research in whole genome selection and applies these discoveries to the aquaculture industry specifically. The text begins with a thorough review of SNP and transitions into topics such as next generation sequencing, EST data mining, SNP quality assessment, and whole genome selection principles. Ending with a discussion of the technology's specific applications to the industry, this text will be a valuable reference for those involved in all aspects of aquaculture research. Special Features:
|
|
publisher: Humana Press, published: 2009-10-27 ASIN: 1603274103 EAN: 9781603274104 sales rank: 2132108 In recent years, single nucleotide polymorphisms have received increased and special attention in a rapidly developing field of personalized medicine and drug treatment. Comprising more than eighty percent of all known polymorphisms, single nucleotide polymorphisms (SNPs) are primarily responsible for phenotypic differences between individuals, and have been suggested to affect the development of diseases in humans and the response to drug treatment and environmental stress. In Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition, expert researchers explore the latest advances in this area, highlighting the substantial progress that has been made in SNP genotyping, examining recent developments in high-throughput genotyping approaches, and exploring our new understanding of the impact of SNPs on gene function. Chapters address the impact of SNPs on phenotype, examine SNP databases, look at methods that have been applied for SNP bioinformatics discovery and analysis, and discuss advanced experimental approaches used for SNP detection. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Current and innovative, Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition is an essential guidebook for individual researchers as well as institutions and companies working in the field. |
|
publisher: Humana Press, published: 2002-10-07 ASIN: 0896039684 EAN: 9780896039681 sales rank: 3583066 Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection. |
|
by: Sebastian Wernicke publisher: Diplomarbeiten Agentur diplom.de, published: 2003-01-01 ASIN: 3838674812 EAN: 9783838674810 sales rank: 6545688 Diplomarbeit, die am 23.09.2003 erfolgreich an einer Universität in Deutschland im Fachbereich Informatik 17 eingereicht wurde. Abstract: This work brings together two areas of science-biology and informatics-that have only recently been connected in the emerging (and vastly growing) research field of Bioinformatics. In order to achieve a common basis for Parts 2 and 3 of this work, Part 1 intends to introduce the computer scientist to the relevant biological background and terminology (Chapter 2), and to familiarize the biologist with the relevant topics from theoretical computer science (Chapter 3). Chapter 2 first introduces some terminology from the field of genetics, thereby defining SNPs. We then motivate the analysis of SNPs by two applications, i.e. the analysis of evolutionary development and the field of pharmacogenetics. Especially the field of pharmacogenetics is capable of having an enormous impact on medicine and the pharmaceutical industry in the near future by using SNP data to predict the efficacy of medication. Chapter 3 gives a brief introduction to the field of computational complexity. We will see and motivate how algorithms are analyzed in theoretical computer science. This will lead to the definition of "complexity classes", introducing the class NP which includes computationally hard problems. Some of the hard problems in the class NP can be solved efficiently using the tool of fixed-parameter tractability, introduced at the end of this chapter. An important application of SNP data is in the analysis of the evolutionary history of species development (phylogenetic analysis - part two - chapters 4 and 5). As will be made plausible in Chapter 5 using SNP data is-in many ways-superior to previous approaches of phylogenetic analysis. In order to analyze the development of species using SNP data, an underlying model of evolution must be specified. A popular model is the so-called perfect phylogeny, but the construction of this phylog... |
|
by: Sio-Iong Ao publisher: Springer, published: 2010-11-05 ASIN: 9048180406 EAN: 9789048180400 Data Mining and Applications in Genomics contains the data mining algorithms and their applications in genomics, with frontier case studies based on the recent and current works at the University of Hong Kong and the Oxford University Computing Laboratory, University of Oxford. It provides a systematic introduction to the use of data mining algorithms as an investigative tool for applications in genomics. Data Mining and Applications in Genomics offers state of the art of tremendous advances in data mining algorithms and applications in genomics and also serves as an excellent reference work for researchers and graduate students working on data mining algorithms and applications in genomics. |
|
publisher: Springer, published: 2008-03-11 ASIN: 3540728643 EAN: 9783540728641 sales rank: 1932048 The carbon nanotubes field has evolved substantially since the publication of the bestseller Carbon Nanotubes: Synthesis, Structure, Properties and Applications . The present volume builds on the generic aspects of the aforementioned book, which emphasizes the fundamentals, with the new volume emphasizing areas that have grown rapidly since the first volume, guiding future directions where research is needed and highlighting applications. The volume also includes an emphasis on areas like graphene, other carbon-like and other tube-like materials because these fields are likely to affect and influence developments in nanotubes in the next 5 years. |
|
by: Human Genome Organization (HUGO) publisher: Wiley, published: 2005-02-03 ASIN: 0471234443 EAN: 9780471234449 sales rank: 3063323 As genomic science and potential gene therapies continue to develop, the detection of mutations plays an increasingly central role in diverse areas of biology. With innumerable detection techniques available spanning multiple disciplines, the choice of the best method for a particular study can prove exceedingly difficult. Complexity, equipment requirements, cost, and throughput are all factors to consider, along with the huge amount of information already available through genome sequencing projects. An essential go-to for informed decision-making, the Guide to Mutation Detection is the only single source for the theory and practice of mutation analysis needed to design an experiment. User-friendly, concise, and expertly edited by recognized leaders in the field, the text provides a cutting-edge reference that is still accessible to those with no experience in mutation analysis. The coverage is divided into two sections, with the first section reviewing such topics as: * Key technologies * Mutation scanning * Cleavage * Quality control * High throughput approaches * Databases * Nomenclatures The second section focuses on step-by-step protocols and discussion of methods, including: * Tag-array minisequencing * Electronic hybridization * Pyrosequencing * Fluorescent Single-Stranded Conformation Polymorphism (SSCP) * Denaturing High-Performance Liquid Chromatography (DHPLC) * Array Comparative Genomic Hybridization (ACGH) * Microplate Array Diagonal Gel Electrophoresis (MADGE) Throughout the text, the emphasis remains on practical applications. The detailedprotocols derive from the well-regarded Human Genome Organization (HUGO) training course, particularly those in Montpellier, France, 2002, organized by Mirielle Claustres, Johan den Dunnen, and Graham Taylor. Useful appendices contain important safety data, supplier lists, and bioinformatics resources. Up-to-date, user friendly, and comprehensive, the Guide to Mutation Detection is an invaluable resource for students and practitioners working across a wide range of genetics-related disciplines. |
|
by: Tomoyo Takata publisher: Wayne State University Press, published: 2002-04-01 ASIN: B0008F0HU0 This digital document is an article from Human Biology, published by Wayne State University Press on April 1, 2002. The length of the article is 863 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser. From the author: KEY WORDS: SINGLE NUCLEOTIDE POLYMORPHISM (SNP), MYOGLOBIN, JAPANESE POPULATION Citation Details Title: Allele frequencies of single nucleotide polymorphisms in the second exon of the myoglobin gene among the Japanese. (Brief Communication).(Brief Article)(Statistical Data Included) Author: Tomoyo Takata Publication: Human Biology (Refereed) Date: April 1, 2002 Publisher: Wayne State University Press Volume: 74 Issue: 2 Page: 317(4) Article Type: Brief Article, Statistical Data Included Distributed by Thomson Gale |
|
|
publisher: Biotech Patent News, published: 2002-08-01 ASIN: B0008FFHDW This digital document is an article from BIOTECH Patent News, published by Biotech Patent News on August 1, 2002. The length of the article is 491 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser. Citation Details Title: DNAPrint applies for patent covering 2,425 SNPs.(DNAPrint Genomics, Inc. patents single nucleotide polymorphisms) Publication: BIOTECH Patent News (Newsletter) Date: August 1, 2002 Publisher: Biotech Patent News Volume: 16 Issue: 8 Page: NA Distributed by Thomson Gale |