Related books on 'transgenic transgenesis'
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publisher: Humana Press, published: 2001-12-15 ASIN: 0896036960 EAN: 9780896036963 sales rank: 1527945 This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. |
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by: Louis-Marie Houdebine publisher: Wiley, published: 2003-04-28 ASIN: 0470848286 EAN: 9780470848289 sales rank: 3149749 Animal Transgenesis and Cloning is a concise, balanced introduction to this dynamic subject, covering key issues and current techniques currently used in animal transgenesis and cloning. Whilst providing the reader with the essentials of the subject, from the molecular basis of gene structure and function to therapeutic gene cloning in humans, social and ethical implications of this important area of research are also considered. Written in a clear, accessible style, the book starts with an introduction to key molecular biology techniques and in particular, considers techniques used specifically for cloning animals and generating transgenic animals. Later chapters examine the diverse theoretical, technical and ethical issues raised by cloning and transgenesis in both animals and humans. Animal Transgenesis and Cloning: * Is an accessible, concise introduction to this dynamic field * Discusses biological, technical and ethical issues related to cloning and transgenesis * Provides an up-to-date account of the latest research methods and applications. Reviews 'This is a proposal for a book translation in a field that is clearly of importance and very topical. The idea of the book, from gene to transgenic animal, to its uses and abuses is sound and logical ...... It is probable that I would suggest (using) a book similar to the one proposed' Dr Janet Smith, University of Birmingham. 'Chapter one was a tour de force! Very concise and admirably clear ...... I was particularly impressed with the author's 2-page summary of eukaryotic gene regulation, which is no mean achievment!' Dr David de Pomerai, University of Nottingham. '...... I felt that this is a well written, topical book. It covers a lot of ground ...... in my opinion the author manages to provide a satisfying amount of detail and a well focussed treatment of each topic. I like the logic of the book, the fact that it pulls together all of the issues into one readable and manageable text' Dr Brendan Curran, Queen Mary, University of London. |
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publisher: Humana Press, published: 2009-07-30 ASIN: 1603275525 EAN: 9781603275521 sales rank: 1447003 The volume will cover three areas of research in brain tumors: a) animal models for brain tumors, including spontaneous occurrence of brain tumors in mammals, transgenic models in rodents and transplant models; b) Prognostic factors and biomarkers; c) Therapeutic targets and targeting approaches to brain tumors. |
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publisher: Humana Press, published: 2011-01-06 ASIN: 1617374571 EAN: 9781617374579 sales rank: 4684065 For volume 2 alone: A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems. For both volumes: A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. The methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems. |
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publisher: Springer, published: 2010-11-23 ASIN: 3642066658 EAN: 9783642066658 sales rank: 5622687 Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis. |
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publisher: Springer, published: 2001-05-15 ASIN: 0792373448 EAN: 9780792373445 sales rank: 9059587 Transgenic Models in Endocrinology is a fitting addition to the Endocrine Updates series. The aim of these publications is to provide the clinician with cutting edge, yet succinct, access to the latest advances in endocrinology. Current advances in this rapidly evolving area of endocrinology make this a timely and important update for improving our understanding of transgenic models. Transgenic Models in Endocrinology continues the standard of excellence as the thirteenth volume in this series of topical updates. |
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publisher: Humana Press, published: 2009-06-29 ASIN: 1603270183 EAN: 9781603270182 sales rank: 876172 One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques. |
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publisher: Taylor & Francis, published: 2011-06-15 ASIN: 1420093789 EAN: 9781420093780 sales rank: 2968872 As the world debates the risks and benefits of plant biotechnology, the proportion of the global area of transgenic field crops has increased every year, and the safety and value continues to be demonstrated. Yet, despite the success of transgenic field crops, the commercialization of transgenic horticultural crops (vegetables, fruits, nuts, and ornamentals) has lagged far behind. Transgenic Horticultural Crops: Challenges and Opportunities examines the challenges for the creation and commercialization of horticultural biotechnology and identifies opportunities, strategies, and priorities for future progress. A "must read" for anyone working in the fields of genetic engineering or plant breeding, for policy makers, educators, students, and anyone interested in the issues of genetic engineering of fruits, vegetables and ornamentals, this book covers:
The production and commercialization of transgenic horticultural crops is an enormous task—its progress and realization require an informed research community, horticultural industry, government, and body of consumers. To aid in this effort, this book provides facts, analyses and insights by leading experts in this field to inform a wide audience of students, agricultural and genetic professionals, and the interested public. Part of the global conversation on the pros and cons of transgenic foods, Transgenic Horticultural Crops aims to stimulate more interest and discussion on the subject and to promote the development of safe and sustainable genetically modified horticultural crop varieties. |
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publisher: Cambridge University Press, published: 2005-01-17 ASIN: 0521836778 EAN: 9780521836777 sales rank: 930376 RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this 2005 overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference. |
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publisher: Springer, published: 2006-07-28 ASIN: 3540442448 EAN: 9783540442448 sales rank: 1521335 Here is a broad overview of the central topics and issues in molecular biology and molecular medicine, with up-to-the minute information about developments in the field including pharmacogenics and pharmacoproteomics, gene therapy and gene regulation. Presented in an accessible A to Z format, the Encyclopedia’s more than 2000 entries are written by leading experts in genomics and proteomics. The entries comprise in-depth essays, illustrated with full-color figures, and presented in a lucid style that will appeal to both experts and interested lay people. |