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publisher: Humana Press, published: 2009-03-05
ASIN: 1934115495
EAN: 9781934115497
sales rank: 951860
As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
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publisher: Humana Press, published: 2010-11-17
ASIN: 1607619733
EAN: 9781607619734
sales rank: 1324287
The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Transgenic Mouse Methods and Protocols, second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.
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publisher: Routledge, published: 2007-07-23
ASIN: 0415380103
EAN: 9780415380102
sales rank: 6151505
Genetic engineering is changing the terrain of development studies. Technologies with unprecedented potential - the capacity to move genes across species - have created widely politicized phenomena: ‘Frankenfoods’, ‘GMOs’, and ‘The Terminator’. En masse, the public has reacted with equanimity or appreciation to genetically engineered pharmaceuticals, beginning with insulin, but transgenics in food and agriculture have raised a globally contentious politics. This book begins with the needs of the poor - for income, nutrition, environmental integrity - and evaluates the theory and evidence for contributions from transgenic crops. Social scientists with expertise in regional studies, economics, sociology, agriculture and political science join biologists to bring specialized knowledge on genuinely new questions created by the genomics revolution; questions of: - ecological integrity
- biodiversity
- international trade
- the costs and effectiveness of biosafety protocols.
The authors collectively conclude that predictions of disaster for the poor from transgenic technology are uninformed by empirical results, rest on misunderstandings of biotechnology or the poor or both, or get the science wrong. Yet the triumphalism of pro-transgenic forces, however, must be tempered by serious unanswered questions: much is unknown, but the transgenic genie is out of the bottle. In this much-needed book, an emergent empirical literature allows scholars in disciplines ranging from micro-biology to economics and political science to assess the potential effects of transgenic organisms on poverty through multiple dynamics of property, yields, prices, biodiversity, environmental integrity and nutrition.
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publisher: Birkhäuser Basel, published: 1999-12-09
ASIN: 3764361832
EAN: 9783764361839
The Berne Symposium invited leading scientists of risk assessment research with transgenic crops on an international level in order to enhance the discussion regulators and members of the biotech industry. The goal was to determine the status quo and also to make progress in times of a first global spread of transgenes in agrosystems about risk assessment. The dialogue between scientists, regulators and industry representatives also revealed some lacunes of risk assessment research, which will have to be filled in the future: We still lack longterm experience, for which we will have to collect data with scientific precision. The symposium concluded asking for a risk-oriented longterm monitoring system based on critical science and hard data. This volume presents the discussion sessions as well as the scientific contributions and thus mirrors the risk assessment debate, based not on exaggerated negative scenarios but on critical science and hard data.
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publisher: Birkhäuser Basel, published: 2004-04-20
ASIN: 3764370564
EAN: 9783764370565
The set consists of 4 Volumes and has the aim to compile a catalogue of test methods useful for the risk assessment of transgenic plants. Vol. 1: Competition, Establishment and Ecosystem Effect Vol. 2: Pollination, Gene-Transfer and Population Impacts Vol. 3: Ecological risks and prospects of transgenic plants, where do we go from here? A dialogue between biotech industry and science Vol. 4: IV. Biodiversity and Biotechnology
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by: Elizabeth Fitting
publisher: Duke University Press Books, published: 2010-12-31
ASIN: 0822349566
EAN: 9780822349563
sales rank: 628738
When scientists discovered transgenes in local Mexican corn varieties in 2001, their findings intensified a debate about not only the import of genetically modified (GM) maize into Mexico but also the fate of the peasantry under neoliberal globalization. While the controversy initially focused on the extent to which gene flow from transgenic to local varieties threatens maize biodiversity, anti-GM activists emphasized the cultural significance of the crop in Mexico and demanded that campesinos and consumers have a voice in the creation of GM maize and rural policies. In The Struggle for Maize, Elizabeth Fitting explores the competing claims of the GM corn debate in relation to the livelihood struggles of small-scale maize producers, migrants, and maquiladora workers from the southern Tehuacán Valley. She argues that the region’s biodiversity is affected by state policies that seek to transform campesinos into entrepreneurs and rural residents into transnational migrant laborers. While corn production and a campesino identity remain important to an older generation, younger residents have little knowledge of or interest in maize agriculture; they seek out wage labor in maquiladoras and the United States. Fitting’s ethnography illustrates how agricultural producers and their families respond creatively to economic hardship and Mexico’s “neoliberal corn regime,” which promotes market liberalization, agricultural “efficiency,” and the reduction of state services over domestic maize production and food sovereignty.
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by: Hugh Lacey
publisher: Lexington Books, published: 2005-08
ASIN: 0739111418
EAN: 9780739111413
sales rank: 2105762
Values and Objectivity in Science illuminates many of the ethical issues that arise concerning scientific practices and applications, offering an account of how social and ethical values play important roles within science. Hugh Lacey develops and clarifies his previous analysis by arguing for the importance of research being conducted under a plurality of strategies, contrasting 'materialist strategies' with 'agro-ecological strategies.' By displaying the structure of current ethical controversies about the legitimacy of planting transgenic crops, this book illustrates that sound thinking on such issues must be grounded on an adequate philosophy of science, one that can clearly distinguish between the proper and the distorting roles of values in scientific practices. This book will prove useful for science students and practitioners as well as those interested in the growing ethical questions involved in scientific practices.
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by: Jacqueline N. Crawley PhD
publisher: Wiley-Liss, published: 2007-03-23
ASIN: 0471471925
EAN: 9780471471929
sales rank: 359052
Dr. Jacqueline N. Crawley, author of the First and Second Editions of What’s Wrong with My Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice,continues to field calls and e-mails from molecular geneticists who ask: how do I run behavioral assays to find out what’s wrong with my mouse?Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions. The final chapter, "The Next Generation," examines new and emerging technologies. Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers.
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by: Louis-Marie Houdebine
publisher: CRC Press, published: 1997-05-24
ASIN: 9057020696
EAN: 9789057020698
sales rank: 4270345
During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues.
Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics.
Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.
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by: Budd Hopkins
publisher: Pocket Star, published: 2004-09-28
ASIN: 0743412192
EAN: 9780743412193
sales rank: 1011695
The New York Times bestselling author of Witnessed, Intruders, and Missing Time -- three groundbreaking books on the UFO phenomenon -- returns with astonishing evidence that other-worldly beings are a very real -- and growing -- part of our lives. In Sight Unseen, Budd Hopkins and coauthor Carol Rainey show how fascinating discoveries in modern science support the plausibility of the UFO phenomenon. Featuring sixteen never-before-published cases, Sight Unseen probes two newly uncovered patterns in alien abduction: cases of UFO "invisibility" and reports of genetically altered alien beings who interact with humans during their routine lives. The "invisibility" accounts detailed by Hopkins include numerous daylight abductions in densely populated urban areas -- all apparently unseen and accomplished through a technology of invisibility. - Two air force non-coms are snatched from the tarmac of a busy military airfield.
- An Australian family is levitated up into a hovering craft while the father remains paralyzed on the ground with a camera to his eye. The resulting evidence on film is discussed in terms of our own scientific advances.
In the second series of cases, abductees report encounters with beings who appear human but apparently possess paranormal powers and stunted emotional ranges. - Three young women, unknown to each other, are mysteriously summoned to "job interviews." In ordinary office settings, they encounter human-looking beings who lead them into baffling UFO abduction experiences.
- A Wisconsin farmer meets "Damoe," a man with odd behavior who closely resembles his son. Damoe eventually reveals himself as an accomplice of UFO occupants in a startling abduction of the farmer and his wife.
- Five-year-old Jen is abducted at night to a nearby playground. There she must teach the techniques and skills of "play" to twelve seemingly identical, quasi-human children.
Along with these bizarre, first-person stories told by credible people, Hopkins and Rainey explore cutting-edge advances in our own technologies and scientific theories that show how these new UFO patterns could have a concrete basis in contemporary science. Included are an examination of cloaking devices for aircraft, mind-control technologies, and teleportation achieved in the lab. Perhaps the most compelling argument to support these cases lies in the startling and controversial new science of transgenics that actually allows for the creation of alien/human beings.
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