Related books on 'virus-protocol'
Return to life science book listNumber of products: 639
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publisher: Springer, published: 2012-03-31 ASIN: 1617796204 EAN: 9781617796203 sales rank: 692063 |
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publisher: Humana Press, published: 2011-09-27 ASIN: 1617793396 EAN: 9781617793394 sales rank: 1234550 Since the first report of an engineered oncolytic virus, there has been a continuing and steady increase of interest in the field, and while bench research remains vital for the translation of research in this field, its success depends on breakthroughs in clinical studies. Oncolytic Viruses: Methods and Protocols describes the construction and purification of capsid-modified adenoviruses as well as oncolytic adenoviruses, presents protocols for many individual virus species including engineering and preparation of oncolytic HSV, propagation, purification, and in vivo testing of oncolytic VSV, details properties of oncolytic reovirus and NDV, and describes the generation and testing of next generation of oncolytic vaccinia virus. As the host immune system plays a critical role in determining efficacy of oncolytic viruses, two chapters are devoted to the study of immune response. Recent advances in stem cell research have led the field in two distinct directions: the use of stem cells as carrier vehicles for oncolytic viruses and the targeting of cancer stem cells. As such, the volume describes the use of explant tissue samples from patients to potentially provide useful information predicting responses prior to clinical translation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Oncolytic Viruses: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential and vital field. |
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publisher: Humana Press, published: 2010-11-09 ASIN: 1617370134 EAN: 9781617370137 sales rank: 11135108 An outstanding collection of state-of-the-art experimental methods for herpes virology. These procedures range from the more biological in vivo maneuvers to the purely molecular, and are described in detail by selected experts to ensure reproducibility. Most of the protocols rely on the herpes simplex virus as prototype, so that it then becomes relatively easy to extrapolate and make the necessary modifications required for application to other herpesviruses, especially members of the alpha group, such as PRV and EHV. |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617371378 EAN: 9781617371370 sales rank: 4176684 Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer. |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617374377 EAN: 9781617374371 sales rank: 1627823 A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer. |
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by: Donald Correll publisher: Acute Care Horizons, LLC, published: 2010-11-15 ASIN: 0982819110 EAN: 9780982819111 sales rank: 731302 The first compendium of acute care protocols created for the Physician assistant. Over 130 concise, fast-reading, bulleted protocols for Physician assistants working in acute care within an emergency department, urgent care center, family or office practice. Covers cardiovascular, respiratory, endocrine, toxicology, neurology, musculoskeletal, gastrointestinal, genitourinary disorders, electrolyte and acid/base disturbances, HEENT, trauma, pediatrics, geriatrics, gynecology, infectious disease, dermatology, hematology, psychiatric and social, environmental, medications, and disease management, among others. This book has evidenced-based care and best practices, provides a framework for the Physician assistant interaction with physicians, and is a Risk Management tool to decrease adverse outcomes. The Protocols and Practice Guide have similar clinical content --- The author Donald Correll, M.D. is the Emergency Department Medical Director of Jackson-Madison County General Hospital (Tennessee), which treats 85,000 patients annually. |
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publisher: Humana Press, published: 1999-03-15 ASIN: 0896033694 EAN: 9780896033696 sales rank: 2726836 This collection of cutting-edge techniques is essential for studying the molecular biology, virology, and immunology of the HIV virus. The methods cover a broad range of research interests, including quantitation of viral genomes, HIV promoter function, B-cell epitope mapping, viral coreceptor usage, and measurements of T-cell function. Special emphasis is accorded to studying those viral and host immune responses to infection that will be critical to the design of effective preventive vaccines. HIV Protocols is the first HIV methods book to concentrate equally on virology, molecular biology, and immunology, as well as to incorporate methods on chemokine receptor structure and function. |
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publisher: Humana Press, published: 1998-02-24 ASIN: 0896033856 EAN: 9780896033856 sales rank: 1881235 A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. |
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publisher: Humana Press, published: 1998-09-18 ASIN: 0896035212 EAN: 9780896035218 sales rank: 4386091 Johnson Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus, a disease afflicting over 100 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue to HCV genotypes and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the introduction of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. |
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publisher: Humana Press, published: 2004-10-22 ASIN: 158829353X EAN: 9781588293534 sales rank: 2635820 A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease. |