|
by: Dany Spencer Adams
publisher: Cold Spring Harbor Laboratory Press, published: 2003-09-01
ASIN: 0879696346
EAN: 9780879696344
sales rank: 206825
Work at the biology bench requires an ever-increasing knowledge of mathematical methods and formulae. In Lab Math, Dany Spencer Adams has compiled the most common mathematical concepts and methods in molecular biology, and provided clear, straightforward guidance on their application to research investigations. Subjects range from basics such as scientific notation and measuring and making solutions, to more complex activities like quantifying and designing nucleic acids and analyzing protein activity. Tips on how to present mathematical data and statistical analysis are included. A reference section features useful tables, conversion charts and "plug and chug" equations for experimental procedures. This volume is an excellent, structured source of information that in many laboratories is often scattered and informally organized. Related Titles from the Publisher Statistics at the Bench: A Step-By-Step Handbook for Biologists Lab Dynamics At the Bench At the Helm Lab Ref, Volume 2: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench
|
|
by: Kathy Barker
publisher: Cold Spring Harbor Laboratory Press, published: 2005-01-01
ASIN: 0879697083
EAN: 9780879697082
sales rank: 58571
At the Bench is the unique and hugely successful handbook for living and working in the laboratory, an essential aid to understanding basic lab techniques and how research groups work at a human level. In this newly revised edition, chapters have been rewritten to accommodate the impact of computer technology and the Internet, not only on the acquisition and analysis of data, but also on its organization and presentation. Alternatives to the use of radiation have been expanded, and figures and illustrations have been redrawn to reflect changes in laboratory equipment and procedures. Related Titles from the Publisher At the Helm: A Laboratory Navigator Lab Ref, Volume 2: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench Lab Dynamics Lab Math
|
|
publisher: CRC Press, published: 2011-12-12
ASIN: 1420069381
EAN: 9781420069389
sales rank: 452231
Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.
|
|
publisher: Humana Press, published: 2009-05-15
ASIN: 1934115738
EAN: 9781934115732
sales rank: 1237934
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols", expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.
|
|
by: Clifton E. Meloan
publisher: Wiley-Interscience, published: 1999-10-06
ASIN: 0471351970
EAN: 9780471351979
sales rank: 2857214
Performing effective chemical separations-a step-by-step guide to the most commonly used techniques. How do experienced analysts go about making a chemical separation work? Through precise, detailed coverage of the principles, equipment, and techniques involved, this combination laboratory manual and reference source gives readers a working knowledge of an impressive array of separation methods. In forty-two chapters, it explores all major categories of separation, including those involving phase changes, extraction, chromatography, ion-exchange resins, electric fields, flotation, membranes, and miscellaneous techniques. With an emphasis on everyday practice rather than theory, Chemical Separations explains the principles and parameters of these methods with a minimum of mathematics, while providing 59 specific experiments to demonstrate proper procedures. Drawn from well-known commercial and academic laboratories and approved by national standard-setting organizations, these experiments feature step-by-step protocols for each separation scheme, precise instructions on setting up the apparatus, and helpful checklists for essential chemicals and supplies. With Chemical Separations as their guide, laboratory analysts and newcomers to chemical analysis will learn how to obtain quality analysis using commercial products, natural samples, and proven real-world laboratory techniques.
|
|
by: D C Darling
publisher: Garland Science, published: 1994-01-01
ASIN: 0199634467
EAN: 9780199634460
sales rank: 3984333
Discusses very widely-used techniques. Aimed specifically at the newcomer. Provides detailed explanations unavailable elsewhere. Points out pitfalls and provides solutions. Invaluable help for experienced laboratories training newcomers. Foreward wri
|
|
publisher: Academic Press, published: 1995-06-05
ASIN: 0124262910
EAN: 9780124262911
sales rank: 6471207
Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols.
Key Features * Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules * Gives protocols for nonisotopic DNA sequencing - new in this edition * Gives extensive, practical information * Presents background information for each method * Provides expert accounts from the inventor or developer of each method * Contains seven entirely new chapters * Covers all major types of nonisotopic procedures for labeling and detection
|
|
by: Mario De Caro
publisher: Springer, published: 1999-09-30
ASIN: 0792358694
EAN: 9780792358695
sales rank: 4813943
Many articles and books dealing with Donald Davidson's philosophy are dedicated to the papers and ideas Davidson put forward in the `sixties and `seventies. In the last two decades, however, Davidson has continued to work in many areas of philosophy, offering new contributions, many of which are highly regarded by philosophers working in the fields concerned. For instance, Davidson has considerably developed his ideas about interpretation, theory of meaning, irreducibility of the mental, causation, and action theory; he has proposed an innovative externalist conception of the mental content and a new analysis of the concept of truth; and he has partly modified his theses about event, and the supervenience of the mental on the physical. In Interpretations and Causes, some of the leading contemporary analytic philosophers discuss Davidson's new ideas in a lively, relevant, useful, and not always entirely sympathetic way. Davidson himself offers and original contribution.
|
|
by: William Wu
publisher: CRC Press, published: 2003-09-29
ASIN: 0849312884
EAN: 9780849312885
sales rank: 4106318
Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development.
Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects.
Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.
|
|
by: William Wu
publisher: CRC Press, published: 2011-05-18
ASIN: 1439848300
EAN: 9781439848302
sales rank: 1727833
Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book: - Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies
- Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips
- Addresses the needs of researchers in academic and commercial environments
- Guides graduate students in designing, implementing, and evaluating experimental projects.
Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.
|