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by: Steven A. Williams
publisher: Jones & Bartlett Learning, published: 2006-08-11
ASIN: 0763733296
EAN: 9780763733292
sales rank: 816177
Laboratory Investigations In Molecular Biology Describes Well-Tested Protocols For Procedures In Molecular Biology That Are Commonly Used In Currently Active Research Labs. The Experiments Are Designed To Mimic Real Investigations And To Provide Students With Instruction In Methods And Approaches That An Be Immediately Translated To Real Research Projects In Real Research Laboratories. A Key And Unique Feature Of The Laboratory Text Is That It Is Supported By The Availability Of Prepackaged And Pretested Kits Containing Reagents Required For Each Module In Each Project, Which Will Ensure Fail-Safe Function Of The Reagents For Each Experiment.
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by: Jonathan M. W. Slack
publisher: Wiley-Blackwell, published: 2005-10-17
ASIN: 1405122161
EAN: 9781405122160
sales rank: 427289
TO ACCESS THE DEDICATED TEXTBOOK WEBSITE, PLEASE VISIT www.blackwellpublishing.com/slack
Essential Developmental Biology, 2nd Edition, is a concise and well-illustrated treatment of this subject for undergraduates. With an emphasis throughout on the evidence underpinning the main conclusions, this book is suitable as the key text for both introductory and more advanced courses in developmental biology.
- Includes new chapters on Evolution & Development, Gut Development, & Growth and Aging.
- Contains expanded treatment of mammalian fertilization, the heart and stem cells.
- Now features a glossary, notated further reading, and key discovery boxes.
- Illustrated with over 250 detailed, full-color drawings.
- Accompanied by a dedicated website, featuring animated developmental processes, a photo gallery of selected model organisms, and all art in PowerPoint and jpeg formats (also available to instructors on CD-ROM).
An Instructor manual CD-ROM for this title is available. Please contact our Higher Education team at HigherEducation@wiley.com for more information.
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publisher: Springer, published: 2012-03-31
ASIN: 1617796204
EAN: 9781617796203
sales rank: 705544
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publisher: Humana Press, published: 1993-12-01
ASIN: 0896032477
EAN: 9780896032477
sales rank: 1431048
Leading researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The protocols presented cover all DNA analogs that have been studied to date and constitute the single most extensive source of knowledge about DNA chemistry available.
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publisher: Wiley, published: 2006-03-22
ASIN: 0470847581
EAN: 9780470847589
sales rank: 803771
As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology.
This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.
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publisher: Humana Press, published: 2010-11-19
ASIN: 1617379107
EAN: 9781617379109
sales rank: 532672
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
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publisher: Humana Press, published: 2010-11-19
ASIN: 1617375101
EAN: 9781617375101
sales rank: 3330548
A diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods for studying B-lymphocyte development and function in higher animals. These readily reproducible protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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publisher: Humana Press, published: 2008-07-25
ASIN: 1588295745
EAN: 9781588295743
sales rank: 1512297
Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive “manual” in a field that has become as broad and deep as cardiovascular medicine, this volume of “Methods in Molecular Medicine” covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.
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publisher: Springer, published: 2012-03-31
ASIN: 1617796085
EAN: 9781617796081
sales rank: 896461
Molecular cloning and DNA-based analysis have become part of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is virtually part of every variation of the plethora of approaches used for DNA fingerprinting today. Plant DNA Fingerprinting: Methods and Protocols aims to bring together the different currently available genome-based techniques into one repository. This volume contains detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, the use of DNA barcoding, as well as methods for the bioinformatic analysis of data. Also included are several discussions on the broader issues of genome-based approaches in order to provide a sound understanding of the principles of these methods. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant DNA Fingerprinting: Methods and Protocols is tailored principally for those who seek to augment their current methods of plant analysis and quality control using genome-based approaches as well as for scientists and researchers in different plant sciences.
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publisher: Humana Press, published: 2010-11-19
ASIN: 1617375721
EAN: 9781617375729
sales rank: 516999
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
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