Related books on 'cell-culture tissue-culture'
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by: Michael Butler publisher: Taylor & Francis, published: 2004-03-10 ASIN: 1859960499 EAN: 9781859960493 sales rank: 994996 Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use. |
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by: Craig E. Greene DVM MS DACVIM publisher: Saunders, published: 2011-12-05 ASIN: 1416061304 EAN: 9781416061304 sales rank: 226848 The definitive reference for the diagnosis, treatment, and prevention of small animal infections, Infectious Diseases of the Dog and Cat, 4th Edition delivers current, comprehensive information essential to the management of infectious diseases caused by viruses, rickettsiae, chlamydiae, mycoplasmas, bacteria, fungi, algae, protozoa, and unknown agents. Each section guides you through diagnostic testing for specific microorganisms, from sample collection to laboratory submission to interpretation of results, then details appropriate treatment measures and pharmacologic considerations for the various related infections. Full-color illustrations and hundreds of tables provide quick, convenient access to diagnostic and therapeutic guidelines, and thoroughly updated drug information helps you confidently administer appropriate dosages for the most effective treatment and prevention.
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publisher: Humana Press, published: 1992-08-01 ASIN: 0896032280 EAN: 9780896032286 sales rank: 707045 Tissue culture has become an indispensable tool in the realm of biomedical research, finding widespread use in clinical diagnosis, toxicology, and industrial biotechnology. Developments in tissue culture technology will continue to have a great impact on many other areas in the foreseeable future. In order to work effectively in neuroscience today, it is essential that every biomedical researcher be well-versed in tissue culture technology. Protocols for Neural Cell Culture provides a practical tutorial on tissue culture techniques that every neuroscientist needs to master. Highlighting the best and latest methods, Fedoroff and Richardson have selected only proven protocols that are routinely used in major laboratories, and in most cases, those which they and their colleagues have employed successfully for many years. Experts on neural tissue culture have contributed well-designed chapters to this volume, presenting information on and procedures for: studying myelination by oligodendrocytes and Schwann cells isolating and growing glial cells, including astrocytes, oligodendrocytes, and microglia quantification of cells in culture and growth assays evaluating neuroactive agents applying neurochemistry to the study of neural cells, identification of antigens, preparation of cultures for electron microscopic analysis, and the care and use of dissecting instruments. An especially helpful feature of Protocols for Neural Cell Culture is the presentation of the protocols as they are used in the authors' laboratories. The book also stresses the importance of sterile techniques in order to minimize the use of antibiotics. Where applicable, a general discussion of the technique is included, based on the extensive experience of the editors, encompassing over 40 years of research and teaching. Comprehensive, thorough, and easy to follow, Protocols for Neural Cell Culture is an outstanding bench- and deskside manual. |
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publisher: Humana Press, published: 2011-04-25 ASIN: 1617790796 EAN: 9781617790799 sales rank: 1493366 With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field. |
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by: Karl-Hermann Neumann publisher: Springer, published: 2010-12-15 ASIN: 364210102X EAN: 9783642101021 sales rank: 3883770 This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even "old hands" in tissue culture should find some challenging ideas to think about. |
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publisher: Springer, published: 2000-12-15 ASIN: 079236225X EAN: 9780792362258 sales rank: 4131354 This book describes all human leukemia-lymphoma cell lines that have been established and that grow continuously under standardised in vitro conditions. These lines are derived from cells belonging to all the major hematopoietic cell lineages, i.e. B- and T-lymphocytes, natural killer cells, granulocytic cells and megakaryocytic cells. The clinical data, the culture conditions and the major phenotypic features of the cell lines are described with citations. This book is the first book describing human leukemia-lymphoma cell lines and will be of interest to scientists involved in the areas of hematology, oncology, immunology, molecular biology and cytogenetics. Cancer Cell Lines, Volumes 1-3: These 3 volumes provide a comprehensive text on the culture of established cell lines from every type of human cancer. The volumes provide a basic manual and reference resource for every cancer research scientist using human cancer cells. |
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publisher: Wiley, published: 2000-08-30 ASIN: 0471852139 EAN: 9780471852131 sales rank: 4389222 Cell and tissue culture is a technique in which plant or animal cells are grown under controlled conditions in the laboratory. This is then used for the analysis of the cells themselves, the assessment of the cell's response to chemicals, or as a tool to produce cellular-derived protein products. This book is a collection of fundamental and specific applied procedures in cell and tissue culture which form the basis of the new medical techniques of tissue engineering and gene therapy. It combines both detailed laboratory procedures and informative overviews. * Provides step-by-step protocols with troubleshooting tips and notes on time considerations. * Main procedures are supplemented by alternative procedures, background information and references. * Experimental examples indicate expected results. |
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publisher: Humana Press, published: 1992-09-09 ASIN: 0896033481 EAN: 9780896033481 sales rank: 1329877 Practical Cell Culture Techniques focuses on the advantages of new tissue culture techniques that enable close and detailed study of many nerve cell types. Neuroscience is one of the key areas whose rapid development has been driven by the proliferation of cell culture methodology; some areas of neuroscience, such as glial cell biology and function, now depend almost entirely on cell culture-based research projects. This latest volume of Humana's renowned Neuromethods series provides practical treatment of the new, powerful methodologies and results including: cell culture techniques specifically designed for the novice • the environmental factors that play a role in tissue culturing • cell markers • nutrition requirements of the different cell types • three-dimensional organotypic slice explant cultures • neuronal cultures • cultures of astrocytes, oligodendrocytes, cerebral capillary endothelium, and other cell types. Boulton, Baker, and Walz's new volume on Practical Cell Culture Techniques is sure to be a major resource for everyone-whether expert or novice-involved in studying the central nervous system on the molecular level. |
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publisher: Oxford University Press, USA, published: 1995-02-09 ASIN: 0199634025 EAN: 9780199634026 sales rank: 2833264 Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students. |
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by: Tissue Culture Association publisher: Liss, published: 1987 ASIN: 0845133047 EAN: 9780845133040 |