cell-culture books

Related books on 'cell-culture'

Return to life science book list
Number of products: 30527
Page 2 of 3053

Protocols for Neural Cell Culture

publisher: Humana Press, published: 1992-08-01
ASIN: 0896032280
EAN: 9780896032286
sales rank: 707045
Tissue culture has become an indispensable tool in the realm of biomedical research, finding widespread use in clinical diagnosis, toxicology, and industrial biotechnology. Developments in tissue culture technology will continue to have a great impact on many other areas in the foreseeable future. In order to work effectively in neuroscience today, it is essential that every biomedical researcher be well-versed in tissue culture technology. Protocols for Neural Cell Culture provides a practical tutorial on tissue culture techniques that every neuroscientist needs to master. Highlighting the best and latest methods, Fedoroff and Richardson have selected only proven protocols that are routinely used in major laboratories, and in most cases, those which they and their colleagues have employed successfully for many years. Experts on neural tissue culture have contributed well-designed chapters to this volume, presenting information on and procedures for: studying myelination by oligodendrocytes and Schwann cells isolating and growing glial cells, including astrocytes, oligodendrocytes, and microglia quantification of cells in culture and growth assays evaluating neuroactive agents applying neurochemistry to the study of neural cells, identification of antigens, preparation of cultures for electron microscopic analysis, and the care and use of dissecting instruments. An especially helpful feature of Protocols for Neural Cell Culture is the presentation of the protocols as they are used in the authors' laboratories. The book also stresses the importance of sterile techniques in order to minimize the use of antibiotics. Where applicable, a general discussion of the technique is included, based on the extensive experience of the editors, encompassing over 40 years of research and teaching. Comprehensive, thorough, and easy to follow, Protocols for Neural Cell Culture is an outstanding bench- and deskside manual.

Basic Cell Culture (Practical Approach)

publisher: Oxford University Press, USA, published: 2002-03-21
ASIN: 0199638535
EAN: 9780199638536
sales rank: 1175192
The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.

Cancer Cell Culture: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2011-04-25
ASIN: 1617790796
EAN: 9781617790799
sales rank: 1493366
With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.

Molecular Biology of the Cell, Fourth Edition

by: Bruce Alberts
publisher: Garland Science, published: 2002-03
ASIN: 0815332181
EAN: 9780815332183
sales rank: 32894
Molecular Biology of the Cell is the classic in-dept text reference in cell biology. By extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and create a coherent framework through which non-expert readers may approach the subject. Written in clear and concise language, and beautifully illustrated, the book is enjoyable to read, and it provides a clear sense of the excitement of modern biology. Molecular Biology of the Cell sets forth the current understanding of cell biology (completely updated as of Autumn 2001), and it explores the intriguing implications and possibilities of the great deal that remains unknown. The hallmark features of previous editions continue in the Fourth Edition. The book is designed with a clean and open, single-column layout. The art program maintains a completely consistent format and style, and includes over 1,600 photographs, electron micrographs, and original drawings by the authors. Clear and concise concept headings introduce each section. Every chapter contains extensive references. Most important, every chapter has been subjected to a rigorous, collaborative revision process where, in addition to incorporating comments from expert reviewers, each co-author reads and reviews the other authors' prose. The result is a truly integrated work with a single authorial voice. Features : - Places the latest hot topics sensibly in context - including genomics, protein structure, array technology, stem cells and genetics diseases. - Incorporates and emphasises new genomic data. - All of molecular biology is brought together into one section (chapters 4-7) covering classically defined molecular biology and molecular genetics. - Two chapters deal exclusively with methods and contain information on the latest tools and techniques. - New chapters on "Pathogens, Infection, and Innate Immunity". - Cell Biology Interactive CD-ROM is packaged with every copy of the book. - Contains over 1,600 illustrations, electron micrographs and photographs, of which over 1,000 are originally conceived by the authors.

Practical Cell Culture Techniques (Neuromethods)

publisher: Humana Press, published: 1992-09-09
ASIN: 0896033481
EAN: 9780896033481
sales rank: 1329877
Practical Cell Culture Techniques focuses on the advantages of new tissue culture techniques that enable close and detailed study of many nerve cell types. Neuroscience is one of the key areas whose rapid development has been driven by the proliferation of cell culture methodology; some areas of neuroscience, such as glial cell biology and function, now depend almost entirely on cell culture-based research projects. This latest volume of Humana's renowned Neuromethods series provides practical treatment of the new, powerful methodologies and results including: cell culture techniques specifically designed for the novice • the environmental factors that play a role in tissue culturing • cell markers • nutrition requirements of the different cell types • three-dimensional organotypic slice explant cultures • neuronal cultures • cultures of astrocytes, oligodendrocytes, cerebral capillary endothelium, and other cell types. Boulton, Baker, and Walz's new volume on Practical Cell Culture Techniques is sure to be a major resource for everyone-whether expert or novice-involved in studying the central nervous system on the molecular level.

Atlas of Human Pluripotent Stem Cells: Derivation and Culturing (Stem Cell Biology and Regenerative Medicine)

publisher: Humana Press, published: 2011-11-18
ASIN: 161779547X
EAN: 9781617795473
sales rank: 653398
Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability. The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cells.

Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology, Vol. 612)

publisher: Humana Press, published: 2009-12-17
ASIN: 1607613611
EAN: 9781607613619
sales rank: 1091071
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of "Natural Killer Cell Protocols: Cellular and Molecular Methods" brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, "Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition" seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.

Cell Phones: Invisible Hazards in the Wireless Age: An Insider's Alarming Discoveries about Cancer and Genetic Damage

by: Dr. George Carlo Dr.
publisher: Basic Books, published: 2002-02-09
ASIN: 078670960X
EAN: 9780786709601
sales rank: 560243

Essential reading for the 100 million Americans currently using wireless phones, this thoroughly researched and documented cautionary work stands alongside of such classics as Silent Spring and The Coming Plague. With news reports proliferating of the possible connection between brain tumors and cell phone use, Dr. George Carlo was hired by the cell phone industry in 1993 to study the safety of its product. In 1999 funds for Dr. Carlo's research were not renewed, and the industry sought to discredit him. Undeterred, Carlo now brings his case to the public with a powerful assessment of the dangers posed by the microwave radiation from cell phone antennas—disruption of the functioning of pacemakers, penetration of the developing skulls of children, compromise to the blood-brain barrier, and, most startlingly, genetic damage that is a known diagnostic marker for cancer—as well as a presentation of safeguards that consumers can implement right now to protect their health. ".…the authors raise serious questions about the integrity of the cell phone industry and the FDA."—San Francisco Chronicle "Extraordinarily informative...[a] captivating story…."—Publishers Weekly

General Techniques of Cell Culture (Handbooks in Practical Animal Cell Biology)

by: Maureen A. Harrison
publisher: Cambridge University Press, published: 1997-10-13
ASIN: 052157496X
EAN: 9780521574969
sales rank: 1543174
Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book.

Animal Cell Culture: A Practical Approach

publisher: Oxford University Press, USA, published: 2000-08-15
ASIN: 0199637962
EAN: 9780199637966
sales rank: 1682495
This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practicing cell culture will benefit greatly from this book.

Page: 1 2 < > 3053

Language for page:
Which server:
Operation:
Product category:
Search parameter:
Search Books:

	Protocols for Neural Cell Culture publisher: Humana Press, published: 1992-08-01 ASIN: 0896032280 EAN: 9780896032286 sales rank: 707045 Tissue culture has become an indispensable tool in the realm of biomedical research, finding widespread use in clinical diagnosis, toxicology, and industrial biotechnology. Developments in tissue culture technology will continue to have a great impact on many other areas in the foreseeable future. In order to work effectively in neuroscience today, it is essential that every biomedical researcher be well-versed in tissue culture technology. Protocols for Neural Cell Culture provides a practical tutorial on tissue culture techniques that every neuroscientist needs to master. Highlighting the best and latest methods, Fedoroff and Richardson have selected only proven protocols that are routinely used in major laboratories, and in most cases, those which they and their colleagues have employed successfully for many years. Experts on neural tissue culture have contributed well-designed chapters to this volume, presenting information on and procedures for: studying myelination by oligodendrocytes and Schwann cells isolating and growing glial cells, including astrocytes, oligodendrocytes, and microglia quantification of cells in culture and growth assays evaluating neuroactive agents applying neurochemistry to the study of neural cells, identification of antigens, preparation of cultures for electron microscopic analysis, and the care and use of dissecting instruments. An especially helpful feature of Protocols for Neural Cell Culture is the presentation of the protocols as they are used in the authors' laboratories. The book also stresses the importance of sterile techniques in order to minimize the use of antibiotics. Where applicable, a general discussion of the technique is included, based on the extensive experience of the editors, encompassing over 40 years of research and teaching. Comprehensive, thorough, and easy to follow, Protocols for Neural Cell Culture is an outstanding bench- and deskside manual.
	Basic Cell Culture (Practical Approach) publisher: Oxford University Press, USA, published: 2002-03-21 ASIN: 0199638535 EAN: 9780199638536 sales rank: 1175192 The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.
	Cancer Cell Culture: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2011-04-25 ASIN: 1617790796 EAN: 9781617790799 sales rank: 1493366 With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.
	Molecular Biology of the Cell, Fourth Edition by: Bruce Alberts publisher: Garland Science, published: 2002-03 ASIN: 0815332181 EAN: 9780815332183 sales rank: 32894 Molecular Biology of the Cell is the classic in-dept text reference in cell biology. By extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and create a coherent framework through which non-expert readers may approach the subject. Written in clear and concise language, and beautifully illustrated, the book is enjoyable to read, and it provides a clear sense of the excitement of modern biology. Molecular Biology of the Cell sets forth the current understanding of cell biology (completely updated as of Autumn 2001), and it explores the intriguing implications and possibilities of the great deal that remains unknown. The hallmark features of previous editions continue in the Fourth Edition. The book is designed with a clean and open, single-column layout. The art program maintains a completely consistent format and style, and includes over 1,600 photographs, electron micrographs, and original drawings by the authors. Clear and concise concept headings introduce each section. Every chapter contains extensive references. Most important, every chapter has been subjected to a rigorous, collaborative revision process where, in addition to incorporating comments from expert reviewers, each co-author reads and reviews the other authors' prose. The result is a truly integrated work with a single authorial voice. Features : - Places the latest hot topics sensibly in context - including genomics, protein structure, array technology, stem cells and genetics diseases. - Incorporates and emphasises new genomic data. - All of molecular biology is brought together into one section (chapters 4-7) covering classically defined molecular biology and molecular genetics. - Two chapters deal exclusively with methods and contain information on the latest tools and techniques. - New chapters on "Pathogens, Infection, and Innate Immunity". - Cell Biology Interactive CD-ROM is packaged with every copy of the book. - Contains over 1,600 illustrations, electron micrographs and photographs, of which over 1,000 are originally conceived by the authors.
	Practical Cell Culture Techniques (Neuromethods) publisher: Humana Press, published: 1992-09-09 ASIN: 0896033481 EAN: 9780896033481 sales rank: 1329877 Practical Cell Culture Techniques focuses on the advantages of new tissue culture techniques that enable close and detailed study of many nerve cell types. Neuroscience is one of the key areas whose rapid development has been driven by the proliferation of cell culture methodology; some areas of neuroscience, such as glial cell biology and function, now depend almost entirely on cell culture-based research projects. This latest volume of Humana's renowned Neuromethods series provides practical treatment of the new, powerful methodologies and results including: cell culture techniques specifically designed for the novice • the environmental factors that play a role in tissue culturing • cell markers • nutrition requirements of the different cell types • three-dimensional organotypic slice explant cultures • neuronal cultures • cultures of astrocytes, oligodendrocytes, cerebral capillary endothelium, and other cell types. Boulton, Baker, and Walz's new volume on Practical Cell Culture Techniques is sure to be a major resource for everyone-whether expert or novice-involved in studying the central nervous system on the molecular level.
	Atlas of Human Pluripotent Stem Cells: Derivation and Culturing (Stem Cell Biology and Regenerative Medicine) publisher: Humana Press, published: 2011-11-18 ASIN: 161779547X EAN: 9781617795473 sales rank: 653398 Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability. The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cells.
	Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology, Vol. 612) publisher: Humana Press, published: 2009-12-17 ASIN: 1607613611 EAN: 9781607613619 sales rank: 1091071 As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of "Natural Killer Cell Protocols: Cellular and Molecular Methods" brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, "Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition" seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.
	Cell Phones: Invisible Hazards in the Wireless Age: An Insider's Alarming Discoveries about Cancer and Genetic Damage by: Dr. George Carlo Dr. publisher: Basic Books, published: 2002-02-09 ASIN: 078670960X EAN: 9780786709601 sales rank: 560243 Essential reading for the 100 million Americans currently using wireless phones, this thoroughly researched and documented cautionary work stands alongside of such classics as Silent Spring and The Coming Plague. With news reports proliferating of the possible connection between brain tumors and cell phone use, Dr. George Carlo was hired by the cell phone industry in 1993 to study the safety of its product. In 1999 funds for Dr. Carlo's research were not renewed, and the industry sought to discredit him. Undeterred, Carlo now brings his case to the public with a powerful assessment of the dangers posed by the microwave radiation from cell phone antennas—disruption of the functioning of pacemakers, penetration of the developing skulls of children, compromise to the blood-brain barrier, and, most startlingly, genetic damage that is a known diagnostic marker for cancer—as well as a presentation of safeguards that consumers can implement right now to protect their health. ".…the authors raise serious questions about the integrity of the cell phone industry and the FDA."—San Francisco Chronicle "Extraordinarily informative...[a] captivating story…."—Publishers Weekly
	General Techniques of Cell Culture (Handbooks in Practical Animal Cell Biology) by: Maureen A. Harrison publisher: Cambridge University Press, published: 1997-10-13 ASIN: 052157496X EAN: 9780521574969 sales rank: 1543174 Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book.
	Animal Cell Culture: A Practical Approach publisher: Oxford University Press, USA, published: 2000-08-15 ASIN: 0199637962 EAN: 9780199637966 sales rank: 1682495 This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practicing cell culture will benefit greatly from this book.