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publisher: Humana Press, published: 2012-07-31
ASIN: 161779970X
EAN: 9781617799709
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publisher: Humana Press, published: 2009-06-29
ASIN: 160327992X
EAN: 9781603279925
sales rank: 2216013
With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
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by: Brian K. Hall
publisher: Academic Press, published: 2005-08-11
ASIN: 0123190606
EAN: 9780123190604
sales rank: 870645
Bones and Cartilage provides the most in-depth review ever assembled on the topic. It examines the function, development and evolution of bone and cartilage as tissues, organs and skeletal systems. It describes how bone and cartilage is developed in embryos and are maintained in adults, how bone reappears when we break a leg, or even regenerates when a newt grows a new limb, or a lizard a tail.
This book also looks at the molecules and cells that make bones and cartilages and how they differ in various parts of the body and across species. It answers such questions as "Is bone always bone?” "Do bones that develop indirectly by replacing other tissues, such as marrow, tendons or ligaments, differ from one another?” "Is fish bone the same as human bone?” "Can sharks even make bone?” and many more.
* Complete coverage of every aspect of bone and cartilage * Full of interesting and unusual facts * The only book available that integrates development and evolution of the skeleton * Treats all levels from molecular to clinical, embryos to evolution * Written in a lively, accessible style * Extensively illustrated and referenced * Integrates analysis of differentiation, growth and patterning * Covers all the vertebrates as well as invertebrate cartilages * Identifies the stem cells in embryos and adults that can make skeletal tissues
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publisher: Academic Press, published: 2009-09-24
ASIN: 0123745993
EAN: 9780123745996
sales rank: 358802
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues which make it the model organism for study of vertebrate development. This book will provide overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. The methods and techniques in this volume were chosen by the editors of Methods in Cell Biology, whose goal was to provide fail-safe methods, tips, and "tricks of the trade” to experienced researchers and more junior members in the lab.
* Provides busy researchers a quick reference for time-tested methods and protocols that really work. * Includes "quick tips and tricks” for each method. * Provides pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error.
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publisher: Academic Press, published: 2011-10-07
ASIN: 0123813204
EAN: 9780123813206
sales rank: 740482
This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.
* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * This volume concentrates on Disease Models and Chemical Screens
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by: Michael T. Ghiselin
publisher: Dover Publications, published: 2003-08-04
ASIN: B006TQYAK0
sales rank: 1606458
This volume surveys the complete range of the Darwin's accomplishments, offering a coherent and consistent treatment of the flow of ideas throughout Darwin's works. Ghiselin constructs a unified theoretical system that explains the major features of Darwin's investigations, evaluating the literature from a historical, scientific, and philosophical perspective.
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by: Mark L. Winston
publisher: Harvard University Press, published: 1991-04-01
ASIN: 0674074092
EAN: 9780674074095
sales rank: 525286
From ancient cave paintings of honey bee nests to modern science's richly diversified investigation of honey bee biology and its applications, the human imagination has long been captivated by the mysterious and highly sophisticated behavior of this paragon among insect societies. In the first broad treatment of honey bee biology to appear in decades, Mark Winston provides rare access to the world of this extraordinary insect. In a bright and engaging style Winston probes the dynamics of the honey bee's social organization. He recreates for us the complex infrastructure of the nest, describes the highly specialized behavior of workers, queens, and drones, and examines in detail the remarkable ability of the honey bee colony to regulate its functions according to events within and outside the nest. Winston integrates into his discussion the results of recent studies, bringing into sharp focus topics of current bee research. These include the exquisite architecture of the nest and its relation to bee physiology; the intricate division of labor and the relevance of a temporal caste structure to efficient functioning of the colony; and, finally, the life-death struggles of swarming, supersedure, and mating that mark the reproductive cycle of the honey bee. The Biology of the Honey Bee not only reviews the basic aspects of social behavior, ecology, anatomy, physiology, and genetics, it also summarizes major controversies in contemporary honey bee research, such as the importance of kin recognition in the evolution of social behavior and the role of the well-known dance language in honey bee communication. Thorough, well-illustrated, and lucidly written, this book will for many years be a valuable resource for scholars, students, and beekeepers alike.
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publisher: Academic Press, published: 2011-12-14
ASIN: 012544172X
EAN: 9780125441728
sales rank: 1206199
An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
*Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come * Chapters written by leaders in the field * Tried and true methods deliver busy researchers a one-stop compendium of essential protocols
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publisher: Humana Press, published: 2005-12-13
ASIN: 1588293629
EAN: 9781588293626
sales rank: 1659507
A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.
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publisher: Humana Press, published: 2012-01-21
ASIN: 1617795267
EAN: 9781617795268
sales rank: 1082855
The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes.
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