Related books on 'electrophoresis'
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publisher: Humana Press, published: 2012-02-07 ASIN: 1617795720 EAN: 9781617795725 sales rank: 1072466 Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past and future advances of protein purification and analysis methods. DIGE, being able to quantify proteins in their intact form, is one of a few methods that can facilitate this type of analysis and still provide the protein isoforms in an MS-compatible state for further identification and characterization with high analytical sensitivity. Differential Gel Electrophoresis: Methods and Protocols introduces the concept of DIGE and its advantages in quantitative protein analysis. It provides detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics. Divided into four concise sections, this detailed volume opens with the basics of DIGE, the technique and its practical details with a focus on the planning of a DIGE experiment and its data analysis. The next section introduces various DIGE methods from those employed by scientists world-wide to more novel methods, providing a glance at what is on the horizon in the DIGE world. The volume closes with an overview of the wide range of DIGE applications from Clinical Proteomics to Animal, Plant, and Microbial Proteomics applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Differential Gel Electrophoresis: Methods and Protocols can be used by novices with some background in biochemistry or molecular biology as well as by experts in Proteomics who would like to deepen their understanding of DIGE and its employment in many hyphenations and application areas. With its many protocols, applications, and methodological variants, it is also a unique reference for all who seek fundamental details on the working principle of DIGE and ideas for possible future uses of DIGE in novel analytical approaches. |
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by: Dr Robin Martin publisher: Garland Science, published: 1996-01-01 ASIN: 1872748287 EAN: 9781872748283 sales rank: 3214629 Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes. |
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by: Robert Weinberger publisher: Academic Press, published: 2000-03-01 ASIN: 0127423567 EAN: 9780127423562 sales rank: 2320859 In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Key Features * Illustrated with detailed diagrams of electrophoretic phenomena * Offers step-by-step methods development schemes * Presents techniques for developing quantitative, robust, and precise methods * Includes an extensive troubleshooting guide * Updates and greatly expands on the first edition-more than 50% of the text is new * Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE |
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publisher: Humana Press, published: 2009-04-01 ASIN: 1588299376 EAN: 9781588299376 sales rank: 2427631 Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists. |
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publisher: CRC Press, published: 2007-12-18 ASIN: 0849333296 EAN: 9780849333293 sales rank: 2394359 Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including— Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields. |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617374768 EAN: 9781617374760 Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems. |
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by: Icon Group International publisher: ICON Group International, Inc., published: 2009-07-08 ASIN: 0546962777 EAN: 9780546962772 sales rank: 3211209 Webster's bibliographic and event-based timelines are comprehensive in scope, covering virtually all topics, geographic locations and people. They do so from a linguistic point of view, and in the case of this book, the focus is on "Electrophoresis," including when used in literature (e.g. all authors that might have Electrophoresis in their name). As such, this book represents the largest compilation of timeline events associated with Electrophoresis when it is used in proper noun form. Webster's timelines cover bibliographic citations, patented inventions, as well as non-conventional and alternative meanings which capture ambiguities in usage. These furthermore cover all parts of speech (possessive, institutional usage, geographic usage) and contexts, including pop culture, the arts, social sciences (linguistics, history, geography, economics, sociology, political science), business, computer science, literature, law, medicine, psychology, mathematics, chemistry, physics, biology and other physical sciences. This "data dump" results in a comprehensive set of entries for a bibliographic and/or event-based timeline on the proper name Electrophoresis, since editorial decisions to include or exclude events is purely a linguistic process. The resulting entries are used under license or with permission, used under "fair use" conditions, used in agreement with the original authors, or are in the public domain. |
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by: Margit Burmeister publisher: Humana Press, published: 1991-01-01 ASIN: 0896032299 EAN: 9780896032293 sales rank: 6208831 Burmeister and Ulanovsky's new volume on Pulsed-Field Gel Electrophoresis presents step-by-step protocols for all users of this powerful methodology, whether novice or expert. The book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. Each protocol is given thorough treatment by authors who have had extensive hands-on-experience with it. Indeed, many of the contributors have long provided assistance and troubleshooting advice to new users of PFGE during the early stages of its growth. Here they incorporate the benefits of their experience in the extensive Notes sections, which describe common problems and pitfalls and offer sensible solutions for dealing with them. Topics and techniques treated include: • FIGE, CHEF, OFAGE, TAFE, FORE, ODPFGE • preparation of DNA suitable for PFGE from: bacteria, protozoa, plants, mammals, and yeasts. • preparation and analysis of yeast artificial chromosomes (YACS) • mapping strategies • biological applications • theories and observations. In keeping with the well-known practicality of Humana's Methods in Molecular Biology series, Pulsed-Field Gel Electrophoresis brims with protocols designed to work even the first time they are used. |
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by: Mahmoud H. Hamdan publisher: Wiley-Interscience, published: 2005-02-11 ASIN: 0471648175 EAN: 9780471648178 sales rank: 2471024 The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well. * Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome * Explains the hows and whys of using mass spectrometry in proteomic analysis * Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis * Comments on future challenges and how they may be addressed * Includes sections on troubleshooting |
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by: Dale R. Baker publisher: Wiley-Interscience, published: 1995-03-02 ASIN: 0471117633 EAN: 9780471117636 sales rank: 2773104 The best source of practical, easily accessible information on this exciting new technique CAPILLARY ELECTROPHORESIS Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is fast, sensitive, easy to automate, requires only small sample volumes and reagent amounts, and has been successfully applied to an ever-expanding list of sample types. In Capillary Electrophoresis, analytical practitioners will find a complete, practical guide to the principles, forms, and instrumentation of this technique. The book presents clear and straightforward explanations of the method, its operating principles, and its different modes, including capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary gel electrophoresis, capillary isoelectric focusing, and capillary isotachophoresis. Especially helpful is the material on developing a method: it offers practical guidance on CE modes, capillaries, run buffers, voltage requirements, sample pretreatments, injection modes and amounts, temperature settings, detector selections, buffer replenishment, and data reporting. Capillary Electrophoresis will serve both as an excellent introduction to those who are new to the technique and as a comprehensive reference book to experienced practitioners. A complete and detailed index will assist the reader in quickly finding any topic of interest. |