flow-cytometry books

Related books on 'flow-cytometry'

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Neuro-oncology (NIP- Neurology in Practice)

publisher: Wiley-Blackwell, published: 2012-05-08
ASIN: 0470655755
EAN: 9780470655757
The aim of the Neurology in Practice series is to provide a clinical 'in the office' or 'at the bedside' guide to effective patient care for neurologists in practice and in training. The tone will be practical, not academic. The working assumption is that readers want to know what (and what not) might or should be done, without over emphasis on the why. That said, it is important to review the crucial basic science necessary for effective diagnosis and management, and to provide reminders in the context of the practical chapters.
The books will not be heavily referenced, in line with a more practical approach. This allows for smoother reading (and also relieves the burden of comprehensive citing from authors). Key evidence (clinical trials, Cochrane or other meta analyses) should be summarized in 'Evidence at a Glance' boxes and key references such as reviews, major papers can be provided in the 'selected bibliography' at the end of each chapter.
Practical guidance will be provided through:
the use of algorithms and guidelines where they are appropriate
'Tips and Tricks' boxes – hints on improving outcomes perhaps via practical technique, patient questioning etc
'Caution' warning boxes – hints on avoiding problems, perhaps via contraindications
'Science Revisited' – quick reminder of the basic science principles necessary for understanding

Diagnostic Techniques in Hematological Malignancies

publisher: Cambridge University Press, published: 2010-12-13
ASIN: 0521111218
EAN: 9780521111218
sales rank: 654892
The diagnosis and monitoring of hematological malignancies is complex and requires a systematic approach. Morphology, cell phenotyping, cytogenetics and molecular genetics are essential, and the results must be integrated. Diagnostic Techniques in Hematological Malignancies details the principles and applications of each of these test types in the diagnosis of hematological malignancies in blood and bone marrow. The first section describes the test modalities - including methodological principles, data interpretation and limitations - and is illustrated by clinical examples. The second section focuses on the clinical entities, detailing the most appropriate tests for diagnosis, staging and monitoring of different hematological malignancies and includes test utilization to identify prognostic markers and potential therapeutic targets. With contributions from multiple international experts, this illustrated book is an essential resource for qualified and trainee hematologists, oncologists, and pathologists. It's a practical and useful guide, providing a rational and structured approach to the laboratory assessment of hematological malignancies.

Essential Immunology for Surgeons

by: Oleg Eremin
publisher: Oxford University Press, USA, published: 2011-06-20
ASIN: 019958687X
EAN: 9780199586875
sales rank: 1359742
There has been a huge increase in the knowledge and understanding of the immunological processes involved in surgical practice in recent years. Anaesthesiologists and intensive care physicians are increasingly recognising the significant derangements of immune function in the critically ill patient and the possible therapeutic approaches to manage such patients (including new approaches in transplantation rejections, novel vaccination strategies in cancer treatment, as well as a better understanding of the beneficial effects of standard chemotherapy).

Written by recognised experts in their field from both the UK and the US, and extensively referenced for further reading, Essential Immunology for Surgeons provides a concise, up-to-date, and readable account of immunology in general and its translation into key areas of clinical practice. It provides the necessary foundation for a critical understanding of this essential and rapidly expanding area of biological science that underpins and explains the modern concepts of a range of diseases and conditions, encompassing autoimmunity, infection, trauma, cancer, nutrition, organ transplantation, and various forms of therapy.

Previously published as Immunological Basis for Surgical Science and Practice, this fully-updated textbook is aimed predominantly at the surgical trainee, but will also be of value to undergraduate and postgraduate clinicians of any discipline who wish to have a better understanding and knowledge of the subject, as well as to researchers studying the various core topics discussed in the book.

Flow Cytometry: Principles and Applications

publisher: Humana Press, published: 2010-11-05
ASIN: 1617377201
EAN: 9781617377204
sales rank: 1945716

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers.

The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes [An article from: Analytica Chimica Acta]

by: K. Hibi
publisher: Elsevier, published: 2006-07-28
ASIN: B000PDT8KA
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Listeria monocytogenes can grow at the low temperature commonly used in the storage and transportation of food, and the number of cases of food poisoning caused by L. monocytogenes has increased recently in the US and Europe. Several methods of detecting L. monocytogenes cells have been proposed; however, all existing methods require approximately 48h incubation. In this study, we attempted rapid detection of L. monocytogenes using flow cytometry (FCM). The method is based on measuring the number of L. monocytogenes cells by using a combination of FCM and immunomagnetic separation (IMS). First, polyclonal antibodies (anti-L. monocytogenes rabbit IgG-FITC) conjugated with fluorescein isothiocyanate (FITC) were reacted with L. monocytogenes cells, and then FCM was applied. The cell numbers were determined by FCM using a traditional colony-counting method in the range of 10^4-10^8cellsml^-^1. Tetrameric antibody complexes (TAC) were used because they can recognize both magnetic and FITC molecules on the FITC-conjugated antibodies. FITC-labeled L. monocytogenes cells were reacted with a secondary antibody (TAC) bound to magnetic beads. Then, IMS was used. The method is suitable for detection in the range of 10^2-10^8cellsml^-^1. The FCM assay enumerated the cells within 1min and the total assay time, including sample preparation, was less than 2h.

Stillbirth: Prediction, Prevention and Management

publisher: Wiley-Blackwell, published: 2011-07-12
ASIN: 1444337068
EAN: 9781444337068
sales rank: 2090802
Stillbirth remains a major and tragic obstetric complication
The number of deaths due to stillbirth are greater than those due to preterm birth and sudden infant death syndrome combined.
Stillbirth: Prediction, Prevention and Management provides a comprehensive guide to the topic of stillbirth. Distilling recent groundbreaking research, expert authors consider:
The epidemiology of stillbirth throughout the world
The various possible causes of stillbirth
The psychological effects on mothers and families who suffer a stillbirth
Management of stillbirth
Managing pregnancies following stillbirth
Stillbirth: Prediction, Prevention and Management is packed with crucial evidence-based information and practical insights. It enables all obstetric healthcare providers to manage one of the most traumatic yet all too common situations they will encounter.

Flow Cytometry: A Practical Approach

publisher: Oxford University Press, USA, published: 2000-07-13
ASIN: 0199638241
EAN: 9780199638246
sales rank: 1264293
Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter of immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

Liver Immunology: Principles and Practice

publisher: Humana Press, published: 2007-10-11
ASIN: 1588298183
EAN: 9781588298188
sales rank: 1551960

Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. This volume is a must-read for novice and expert alike. In an easy-to-read and thorough format, it covers bacterial, parasitic and viral infections of the liver, autoimmune liver disease, alcoholic and nonalcoholic fatty liver diseases, and transplantation.

Flow Cytometry and Cell Sorting (Springer Lab Manuals)

publisher: Springer, published: 2010-12-09
ASIN: 3642084923
EAN: 9783642084928
sales rank: 2037266
The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Introduction to Flow Cytometry

by: James V. Watson
publisher: Cambridge University Press, published: 2004-11-11
ASIN: 0521611997
EAN: 9780521611992
sales rank: 1913128
Flow cytometry is a technique for measuring both scattered light and fluorescence from single cells at very rapid rates. Typically up to 5000 cells can be analysed per second. Using various fluorochromes this allows a cell population to be analysed for cells showing certain characteristics such as the presence of a particular enzyme, cellular constituent or other gene product. The information it can provide is invaluable in helping to diagnose certain cancers as well as aiding pure research into many aspects of cell biology such as the cell cycle and gene expression. This book describes the technology in a simple and direct way. The fundamental concepts upon which the technology is based are outlined and the book goes on to describe flow cytometers and what can be gained from using them in biology and medicine. The book gives a particularly detailed account of how artifactual results can arise and where 'noise' is generated. Anyone starting to use, or already using this technique, will need to read this book.

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	Neuro-oncology (NIP- Neurology in Practice) publisher: Wiley-Blackwell, published: 2012-05-08 ASIN: 0470655755 EAN: 9780470655757 The aim of the Neurology in Practice series is to provide a clinical 'in the office' or 'at the bedside' guide to effective patient care for neurologists in practice and in training. The tone will be practical, not academic. The working assumption is that readers want to know what (and what not) might or should be done, without over emphasis on the why. That said, it is important to review the crucial basic science necessary for effective diagnosis and management, and to provide reminders in the context of the practical chapters. The books will not be heavily referenced, in line with a more practical approach. This allows for smoother reading (and also relieves the burden of comprehensive citing from authors). Key evidence (clinical trials, Cochrane or other meta analyses) should be summarized in 'Evidence at a Glance' boxes and key references such as reviews, major papers can be provided in the 'selected bibliography' at the end of each chapter. Practical guidance will be provided through: the use of algorithms and guidelines where they are appropriate 'Tips and Tricks' boxes – hints on improving outcomes perhaps via practical technique, patient questioning etc 'Caution' warning boxes – hints on avoiding problems, perhaps via contraindications 'Science Revisited' – quick reminder of the basic science principles necessary for understanding
	Diagnostic Techniques in Hematological Malignancies publisher: Cambridge University Press, published: 2010-12-13 ASIN: 0521111218 EAN: 9780521111218 sales rank: 654892 The diagnosis and monitoring of hematological malignancies is complex and requires a systematic approach. Morphology, cell phenotyping, cytogenetics and molecular genetics are essential, and the results must be integrated. Diagnostic Techniques in Hematological Malignancies details the principles and applications of each of these test types in the diagnosis of hematological malignancies in blood and bone marrow. The first section describes the test modalities - including methodological principles, data interpretation and limitations - and is illustrated by clinical examples. The second section focuses on the clinical entities, detailing the most appropriate tests for diagnosis, staging and monitoring of different hematological malignancies and includes test utilization to identify prognostic markers and potential therapeutic targets. With contributions from multiple international experts, this illustrated book is an essential resource for qualified and trainee hematologists, oncologists, and pathologists. It's a practical and useful guide, providing a rational and structured approach to the laboratory assessment of hematological malignancies.
	Essential Immunology for Surgeons by: Oleg Eremin publisher: Oxford University Press, USA, published: 2011-06-20 ASIN: 019958687X EAN: 9780199586875 sales rank: 1359742 There has been a huge increase in the knowledge and understanding of the immunological processes involved in surgical practice in recent years. Anaesthesiologists and intensive care physicians are increasingly recognising the significant derangements of immune function in the critically ill patient and the possible therapeutic approaches to manage such patients (including new approaches in transplantation rejections, novel vaccination strategies in cancer treatment, as well as a better understanding of the beneficial effects of standard chemotherapy). Written by recognised experts in their field from both the UK and the US, and extensively referenced for further reading, Essential Immunology for Surgeons provides a concise, up-to-date, and readable account of immunology in general and its translation into key areas of clinical practice. It provides the necessary foundation for a critical understanding of this essential and rapidly expanding area of biological science that underpins and explains the modern concepts of a range of diseases and conditions, encompassing autoimmunity, infection, trauma, cancer, nutrition, organ transplantation, and various forms of therapy. Previously published as Immunological Basis for Surgical Science and Practice, this fully-updated textbook is aimed predominantly at the surgical trainee, but will also be of value to undergraduate and postgraduate clinicians of any discipline who wish to have a better understanding and knowledge of the subject, as well as to researchers studying the various core topics discussed in the book.
	Flow Cytometry: Principles and Applications publisher: Humana Press, published: 2010-11-05 ASIN: 1617377201 EAN: 9781617377204 sales rank: 1945716 Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
	Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes [An article from: Analytica Chimica Acta] by: K. Hibi publisher: Elsevier, published: 2006-07-28 ASIN: B000PDT8KA This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: Listeria monocytogenes can grow at the low temperature commonly used in the storage and transportation of food, and the number of cases of food poisoning caused by L. monocytogenes has increased recently in the US and Europe. Several methods of detecting L. monocytogenes cells have been proposed; however, all existing methods require approximately 48h incubation. In this study, we attempted rapid detection of L. monocytogenes using flow cytometry (FCM). The method is based on measuring the number of L. monocytogenes cells by using a combination of FCM and immunomagnetic separation (IMS). First, polyclonal antibodies (anti-L. monocytogenes rabbit IgG-FITC) conjugated with fluorescein isothiocyanate (FITC) were reacted with L. monocytogenes cells, and then FCM was applied. The cell numbers were determined by FCM using a traditional colony-counting method in the range of 10^4-10^8cellsml^-^1. Tetrameric antibody complexes (TAC) were used because they can recognize both magnetic and FITC molecules on the FITC-conjugated antibodies. FITC-labeled L. monocytogenes cells were reacted with a secondary antibody (TAC) bound to magnetic beads. Then, IMS was used. The method is suitable for detection in the range of 10^2-10^8cellsml^-^1. The FCM assay enumerated the cells within 1min and the total assay time, including sample preparation, was less than 2h.
	Stillbirth: Prediction, Prevention and Management publisher: Wiley-Blackwell, published: 2011-07-12 ASIN: 1444337068 EAN: 9781444337068 sales rank: 2090802 Stillbirth remains a major and tragic obstetric complication The number of deaths due to stillbirth are greater than those due to preterm birth and sudden infant death syndrome combined. Stillbirth: Prediction, Prevention and Management provides a comprehensive guide to the topic of stillbirth. Distilling recent groundbreaking research, expert authors consider: The epidemiology of stillbirth throughout the world The various possible causes of stillbirth The psychological effects on mothers and families who suffer a stillbirth Management of stillbirth Managing pregnancies following stillbirth Stillbirth: Prediction, Prevention and Management is packed with crucial evidence-based information and practical insights. It enables all obstetric healthcare providers to manage one of the most traumatic yet all too common situations they will encounter.
	Flow Cytometry: A Practical Approach publisher: Oxford University Press, USA, published: 2000-07-13 ASIN: 0199638241 EAN: 9780199638246 sales rank: 1264293 Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter of immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.
	Liver Immunology: Principles and Practice publisher: Humana Press, published: 2007-10-11 ASIN: 1588298183 EAN: 9781588298188 sales rank: 1551960 Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. This volume is a must-read for novice and expert alike. In an easy-to-read and thorough format, it covers bacterial, parasitic and viral infections of the liver, autoimmune liver disease, alcoholic and nonalcoholic fatty liver diseases, and transplantation.
	Flow Cytometry and Cell Sorting (Springer Lab Manuals) publisher: Springer, published: 2010-12-09 ASIN: 3642084923 EAN: 9783642084928 sales rank: 2037266 The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS
	Introduction to Flow Cytometry by: James V. Watson publisher: Cambridge University Press, published: 2004-11-11 ASIN: 0521611997 EAN: 9780521611992 sales rank: 1913128 Flow cytometry is a technique for measuring both scattered light and fluorescence from single cells at very rapid rates. Typically up to 5000 cells can be analysed per second. Using various fluorochromes this allows a cell population to be analysed for cells showing certain characteristics such as the presence of a particular enzyme, cellular constituent or other gene product. The information it can provide is invaluable in helping to diagnose certain cancers as well as aiding pure research into many aspects of cell biology such as the cell cycle and gene expression. This book describes the technology in a simple and direct way. The fundamental concepts upon which the technology is based are outlined and the book goes on to describe flow cytometers and what can be gained from using them in biology and medicine. The book gives a particularly detailed account of how artifactual results can arise and where 'noise' is generated. Anyone starting to use, or already using this technique, will need to read this book.