Related books on 'fluorescent-probe'
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publisher: CRC Press, published: 2001-05-30 ASIN: 0849308178 EAN: 9780849308178 sales rank: 9590002 Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cytometry, along with their uses in imaging and quantification fluorescence in in situ hybridization. Written by researchers with extensive experience in the use of photonic microscopy in nucleic acid research, the chapters examine such topics as: fixation; nucleotide substitution; fluorochrome properties; chemically modified nucleotides and methods of incorporation; single cell gel electrophoresis (comet assay) to detect DNA lesions; fluorescent imaging for in situ study of nuclear distribution and colocalization of molecules (FISH mapping); clarification of the fluorescence microscope and light captors; visualization and processing of 3-D data sets; image acquisition and quantification, along with examples; and more. The chapters were each written by authorities on the particular subjects. In a two-column format, the theory and protocol are placed alongside comments and observations resulting from their experience. The comprehensible explanations of concepts together with the detailed procedures make Imaging of Nucleic Acids and Quantitation in Photonic Microscopy indispensable to your understanding and application of these valuable techniques. |
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publisher: Springer, published: 2010-11-19 ASIN: 3642093183 EAN: 9783642093180 sales rank: 5814343 This, the fourth volume in the Springer series on fluorescence, focuses on the fluorescence of nanosystems, polymers and supermolecules, as well as the development and application of fluorescent probes. Aimed at researchers in organic and physical chemistry and in material sciences, emphasis is placed on the fluorescence of artificial and biological nanosystems; single molecule fluorescence and the luminescence of polymers; and micro- and nanoparticles and nanotubes. |
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publisher: Academic Pr, published: 1993-06 ASIN: 0124778291 EAN: 9780124778290 sales rank: 7362997 This book provides both new and existing scientists with an introduction to the practice of optical probe technology, the problems, the pitfalls and limitations likely to be encountered, the application to practical biological questions and the likely areas for future development. The book focuses on the practical hardware requirements and underlying principles of operation, the strategies leading to development of optical probes and their application to living material. |
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publisher: Wiley-Liss, published: 2005-10-28 ASIN: 0471736821 EAN: 9780471736820 sales rank: 1350099 Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection. |
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Near-Infrared Dyes for High Technology Applications (Nato Science Partnership Subseries: 3 (closed)) publisher: Springer, published: 1998-06-30 ASIN: 0792351010 EAN: 9780792351016 sales rank: 3803358 This timely book opens up new avenues in the development of highly sensitive and specific fluorometric and sensor methods based on the NIR laser techniques, allowing detection down to the single molecule level. Most important are NIR laser diodes in combination with NIR dyes, which facilitates the automation and miniaturisation of reliable UV/vis and fluorescence spectroscopic and immunoassay in situ measurements. This permits, for example, analyses of pH values, metal ions, pollutants, membranes, proteins, living cells and the DNA genetic code. Furthermore, the book describes new applications of NIR dyes in high technology areas, such as photochemistry, molecular biology, clinical chemistry, tumour therapy, laser physics, nonlinear optics, laser sensitive optical recording techniques, optical disks, compact disks, laser printers, optical cards, photoengraving, transparent bar coding, forgery prevention, photoresists, spectrally sensitised photographic materials, thermal transfer printing, and heat shielding materials. A compilation of challenging information for scientists and engineers interested in high technology developments and applications. |
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publisher: Springer, published: 2005-01-12 ASIN: 354022338X EAN: 9783540223382 sales rank: 4002817 Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research. |
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by: John W. Thatcher publisher: University of Michigan Library, published: 1959-01-01 ASIN: B0041KL8NI |
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by: Haugland publisher: Molecular Probes, published: 1992-06 ASIN: 9993203939 EAN: 9789993203933 sales rank: 5769041 |
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by: A.L. Tatarets publisher: Elsevier, published: 2006-06-16 ASIN: B000P6NSQM This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: A series of ring-substituted squaraines absorbing and emitting in the red and NIR spectral region was synthesized and their spectral and photophysical properties (quantum yields, fluorescence lifetimes) and photostabilities were measured and compared to Cy5, a commonly used fluorescent label. The absorption maxima in aqueous media were found to be between 628 and 667nm and the emission maxima are between 642 and 685nm. Squaraine dyes exhibit high extinction coefficients (163,000-265,000M^-^1cm^-^1) and lower quantum yields (2-7%) in aqueous buffer but high quantum yields (up to 45%) and long fluorescence lifetimes (up to 3.3ns) in presence of BSA. Dicyanomethylene- and thio-substituted squaraines exhibit an additional absorption around 400nm with extinction coefficients between 21,500 and 44,500M^-^1cm^-^1. These dyes are excitable not only with red but also with blue diode lasers or light emitting diodes. Due to the favourable spectral and photophysical properties these dyes can be used as fluorescent probes and labels for intensity- and fluorescence lifetime-based biomedical applications. |
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by: L. Guo publisher: Elsevier, published: 2007-04-04 ASIN: B000PDYIA0 This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: In this paper, the synthetic route of a potential antitumor reagent, benzo[b][1,10] phenanthrolin-7(12H)-one (BPO), was improved. A sulfonic group was introduced to BPO to form a new compound, 7-oxobenzo[b][1,10]phenan-throline-12(7H)-sulfonic acid (OPSA), in order to enhance its water-solubility. The molecular structure of OPSA has been confirmed by IR, UV, MS, ^1H NMR and elements analysis. It was proved in our experiments that DNA could quench the fluorescence of OPSA and the maximum quenched intensity appeared at 408nm (@l"e"x=284nm). The quenched fluorescence intensity was proportional to the concentration of DNA. Based on this phenomenon, OPSA had been used as the fluorescent probe for detection of calf thymus DNA (ct-DNA) and the corresponding linear response range was from 1.0 to 150.0@mgmL^-^1 and the limit of detection (LOD) was 3.8ngmL^-^1. Its interaction with ct-DNA was investigated by fluorescence, absorption and viscosity measurements. When binding to ct-DNA, OPSA showed obvious fluorescence quenching and the quenched intensity was stable with the presence and absence of NaCl. The absorption spectra of OPSA had no evidence of increasing or decreasing when ct-DNA was added. The viscosity of OPSA and ct-DNA mixture showed no obvious change comparing with the viscosity of ct-DNA along. The results suggested that the interaction between OPSA and ct-DNA was groove binding in nature. Scatchard plots constructed from fluorescence titration data gave a binding constant of 8.9x10^5Lmol^-^1 and a binding site size of 0.35 base pairs per bound drug molecule. |