gene-targeting knockout-gene books

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Our Genes, Our Choices: How genotype and gene interactions affect behavior

by: David Goldman
publisher: Academic Press, published: 2012-05-18
ASIN: 0123969522
EAN: 9780123969521
sales rank: 707593

Are people free to make choices, or do genes determine behavior? Paradoxically, the answer to both questions is "yes," because of neurogenetic individuality, a new theory with profound implications. This theory emerged from gene discoveries by Dr. Goldman and his colleagues which have been recently published in 'Nature'. Genetic studies reveal pathways from DNA to behavior that are helping us understand the diversity of human behavior and behavior of other species.

In this book, the complexity of human behavior and a person's ability to choose is explained as deriving from a relatively small number of genes which direct neurodevelopmental sequence. The author uses judicial, political, medical, and ethical examples to illustrate that this lifelong process is guided by individual genotype, molecular and physiologic principles, as well as by randomness and environmental exposures we choose and do not choose.
Poses and resolves challenges to moral responsibility raised by modern genetics and neuroscience

Preface will be written by a world renowned geneticist or neuroscientist.

Authoritatively written, provides a neurogenetic mechanism for the origins of free will and ability to make moral choices to engage a broad audience of professionals.

Gene Targeting: A Practical Approach

publisher: Oxford University Press, USA, published: 2000-02-15
ASIN: 019963792X
EAN: 9780199637928
sales rank: 1578782
Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.

Conditional Mutagenesis: An Approach to Disease Models (Handbook of Experimental Pharmacology)

publisher: Springer, published: 2007-02-02
ASIN: 3540351086
EAN: 9783540351085
sales rank: 3178355

Leading experts provide timely and comprehensive information on methods for conditional mutagenesis in the mouse (part 1) and their application to model human physiology and pathophysiology (part 2). It illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms. Finally the book considers the development of new drugs to treat them.

The Apo E Gene Diet

by: Pamela McDonald
publisher: Elite Books, published: 2007-11-19
ASIN: 1600700381
EAN: 9781600700385
sales rank: 731419
Do you know your Apo E geneotype? Do you know why it's important? Researchers have discovered that the APO E gene regulates the way your body transports fats and cholesterol, affecting not just weight but also the heart and vascular system, and influencing our patterns of inflammation - one of the key factors in many serious diseases.This is not a traditional diet; instead of taking a one-size-fits-all approach, it supports each individual's unique genetic recipe with the correct matching nutrition. We now know that different genotypes need a different balance of carbohydrate, fat, and protein. This breakthrough book outlines the latest information so you can make appropriate nutritional decisions based on your specific Apo E genotype. In this way, you can take charge of your body's gene-supportive environment for optimum health. The Apo E Gene Diet can be for everyone, but it is specifically designed for a new generation of patients who want to live life to the fullest instead of waiting around for their biology to catch up with them.

Gene Knockout Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2009-03-27
ASIN: 1934115266
EAN: 9781934115268
sales rank: 2006014
With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In "Gene Knockout Protocols, Second Edition", distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, "Gene Knockout Protocols, Second Edition" provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.

Reviews and Protocols in DT40 Research: Subcellular Biochemistry

publisher: Springer, published: 2007-05-07
ASIN: 1402048955
EAN: 9781402048951
sales rank: 3382108
The vertebrate genome DT40 has proven to be a reliable and robust research subject, with fast doubling time, easy clonability and a relatively stable karyotype. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been provided by a number of laboratories will be particularly useful in this regard.

In Vivo Models of Inflammation: Volume 1 (Progress in Inflammation Research)

publisher: Birkhäuser Basel, published: 2006-10-19
ASIN: 3764375191
EAN: 9783764375195
sales rank: 3227638
In Vivo Models of Inflammation (Vol. 1) provides biomedical researchers in both the pharmaceutical industry and academia with a description of the state-of-the-art animal model systems used to emulate diseases with components of inflammation. This second edition acts as a complement to the first, describing and updating the standard models that are most utilized for specific disease areas. New models are included exploring emerging areas of inflammation research.

Lewin's Essential GENES

by: Benjamin Lewin
publisher: Jones & Bartlett Publishers, published: 2009-03-02
ASIN: 0763759155
EAN: 9780763759155
sales rank: 638223
The new edition of Lewin's Essential GENES is the most accessible, student-friendly text of its kind! Completely revised and rewritten, the Second Edition continues to provide students with the latest findings in the field of molecular biology and molecular genetics. An exceptional new pedagogy enhances student learning and helps readers understand and retain key material like never before. New Concept and Reasoning Checks at the end of each chapter section, End of Chapter Questions and Further Readings for each chapter, and several categories of special topics boxes within each chapter expand and reinforce important concepts. The reorganization of topics in this edition allows students to focus more sharply on the key material at hand and improves the natural flow of course material. New end-of-chapter questions reviews major points in the chapter and allow students to test themselves on important course material.

Genes in Conflict: The Biology of Selfish Genetic Elements

by: Austin Burt
publisher: Belknap Press of Harvard University Press, published: 2008-03-15
ASIN: 0674027221
EAN: 9780674027220
sales rank: 717871

In evolution, most genes survive and spread within populations because they increase the ability of their hosts (or their close relatives) to survive and reproduce. But some genes spread in spite of being harmful to the host organism—by distorting their own transmission to the next generation, or by changing how the host behaves toward relatives. As a consequence, different genes in a single organism can have diametrically opposed interests and adaptations.
Covering all species from yeast to humans, Genes in Conflict is the first book to tell the story of selfish genetic elements, those continually appearing stretches of DNA that act narrowly to advance their own replication at the expense of the larger organism. As Austin Burt and Robert Trivers show, these selfish genes are a universal feature of life with pervasive effects, including numerous counter-adaptations. Their spread has created a whole world of socio-genetic interactions within individuals, usually completely hidden from sight.
Genes in Conflict introduces the subject of selfish genetic elements in all its aspects, from molecular and genetic to behavioral and evolutionary. Burt and Trivers give us access for the first time to a crucial area of research—now developing at an explosive rate—that is cohering as a unitary whole, with its own logic and interconnected questions, a subject certain to be of enduring importance to our understanding of genetics and evolution.
(20060428)

Gene Targeting and Embryonic Stem Cells (Advanced Methods)

by: Jim Mcwhir
publisher: Taylor & Francis, published: 2004-03-01
ASIN: 1859963609
EAN: 9781859963609
sales rank: 2365210

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field.

Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.

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	Our Genes, Our Choices: How genotype and gene interactions affect behavior by: David Goldman publisher: Academic Press, published: 2012-05-18 ASIN: 0123969522 EAN: 9780123969521 sales rank: 707593 Are people free to make choices, or do genes determine behavior? Paradoxically, the answer to both questions is "yes," because of neurogenetic individuality, a new theory with profound implications. This theory emerged from gene discoveries by Dr. Goldman and his colleagues which have been recently published in 'Nature'. Genetic studies reveal pathways from DNA to behavior that are helping us understand the diversity of human behavior and behavior of other species. In this book, the complexity of human behavior and a person's ability to choose is explained as deriving from a relatively small number of genes which direct neurodevelopmental sequence. The author uses judicial, political, medical, and ethical examples to illustrate that this lifelong process is guided by individual genotype, molecular and physiologic principles, as well as by randomness and environmental exposures we choose and do not choose. Poses and resolves challenges to moral responsibility raised by modern genetics and neuroscience Preface will be written by a world renowned geneticist or neuroscientist. Authoritatively written, provides a neurogenetic mechanism for the origins of free will and ability to make moral choices to engage a broad audience of professionals.
	Gene Targeting: A Practical Approach publisher: Oxford University Press, USA, published: 2000-02-15 ASIN: 019963792X EAN: 9780199637928 sales rank: 1578782 Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
	Conditional Mutagenesis: An Approach to Disease Models (Handbook of Experimental Pharmacology) publisher: Springer, published: 2007-02-02 ASIN: 3540351086 EAN: 9783540351085 sales rank: 3178355 Leading experts provide timely and comprehensive information on methods for conditional mutagenesis in the mouse (part 1) and their application to model human physiology and pathophysiology (part 2). It illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms. Finally the book considers the development of new drugs to treat them.
	The Apo E Gene Diet by: Pamela McDonald publisher: Elite Books, published: 2007-11-19 ASIN: 1600700381 EAN: 9781600700385 sales rank: 731419 Do you know your Apo E geneotype? Do you know why it's important? Researchers have discovered that the APO E gene regulates the way your body transports fats and cholesterol, affecting not just weight but also the heart and vascular system, and influencing our patterns of inflammation - one of the key factors in many serious diseases.This is not a traditional diet; instead of taking a one-size-fits-all approach, it supports each individual's unique genetic recipe with the correct matching nutrition. We now know that different genotypes need a different balance of carbohydrate, fat, and protein. This breakthrough book outlines the latest information so you can make appropriate nutritional decisions based on your specific Apo E genotype. In this way, you can take charge of your body's gene-supportive environment for optimum health. The Apo E Gene Diet can be for everyone, but it is specifically designed for a new generation of patients who want to live life to the fullest instead of waiting around for their biology to catch up with them.
	Gene Knockout Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2009-03-27 ASIN: 1934115266 EAN: 9781934115268 sales rank: 2006014 With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In "Gene Knockout Protocols, Second Edition", distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, "Gene Knockout Protocols, Second Edition" provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.
	Reviews and Protocols in DT40 Research: Subcellular Biochemistry publisher: Springer, published: 2007-05-07 ASIN: 1402048955 EAN: 9781402048951 sales rank: 3382108 The vertebrate genome DT40 has proven to be a reliable and robust research subject, with fast doubling time, easy clonability and a relatively stable karyotype. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been provided by a number of laboratories will be particularly useful in this regard.
	In Vivo Models of Inflammation: Volume 1 (Progress in Inflammation Research) publisher: Birkhäuser Basel, published: 2006-10-19 ASIN: 3764375191 EAN: 9783764375195 sales rank: 3227638 In Vivo Models of Inflammation (Vol. 1) provides biomedical researchers in both the pharmaceutical industry and academia with a description of the state-of-the-art animal model systems used to emulate diseases with components of inflammation. This second edition acts as a complement to the first, describing and updating the standard models that are most utilized for specific disease areas. New models are included exploring emerging areas of inflammation research.
	Lewin's Essential GENES by: Benjamin Lewin publisher: Jones & Bartlett Publishers, published: 2009-03-02 ASIN: 0763759155 EAN: 9780763759155 sales rank: 638223 The new edition of Lewin's Essential GENES is the most accessible, student-friendly text of its kind! Completely revised and rewritten, the Second Edition continues to provide students with the latest findings in the field of molecular biology and molecular genetics. An exceptional new pedagogy enhances student learning and helps readers understand and retain key material like never before. New Concept and Reasoning Checks at the end of each chapter section, End of Chapter Questions and Further Readings for each chapter, and several categories of special topics boxes within each chapter expand and reinforce important concepts. The reorganization of topics in this edition allows students to focus more sharply on the key material at hand and improves the natural flow of course material. New end-of-chapter questions reviews major points in the chapter and allow students to test themselves on important course material.
	Genes in Conflict: The Biology of Selfish Genetic Elements by: Austin Burt publisher: Belknap Press of Harvard University Press, published: 2008-03-15 ASIN: 0674027221 EAN: 9780674027220 sales rank: 717871 In evolution, most genes survive and spread within populations because they increase the ability of their hosts (or their close relatives) to survive and reproduce. But some genes spread in spite of being harmful to the host organism—by distorting their own transmission to the next generation, or by changing how the host behaves toward relatives. As a consequence, different genes in a single organism can have diametrically opposed interests and adaptations. Covering all species from yeast to humans, Genes in Conflict is the first book to tell the story of selfish genetic elements, those continually appearing stretches of DNA that act narrowly to advance their own replication at the expense of the larger organism. As Austin Burt and Robert Trivers show, these selfish genes are a universal feature of life with pervasive effects, including numerous counter-adaptations. Their spread has created a whole world of socio-genetic interactions within individuals, usually completely hidden from sight. Genes in Conflict introduces the subject of selfish genetic elements in all its aspects, from molecular and genetic to behavioral and evolutionary. Burt and Trivers give us access for the first time to a crucial area of research—now developing at an explosive rate—that is cohering as a unitary whole, with its own logic and interconnected questions, a subject certain to be of enduring importance to our understanding of genetics and evolution. (20060428)
	Gene Targeting and Embryonic Stem Cells (Advanced Methods) by: Jim Mcwhir publisher: Taylor & Francis, published: 2004-03-01 ASIN: 1859963609 EAN: 9781859963609 sales rank: 2365210 Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.