gene-targeting books

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Laboratory Protocols for Conditional Gene Targeting

by: Raul M. Torres
publisher: Oxford University Press, USA, published: 1997-10-02
ASIN: 019963677X
EAN: 9780199636778
sales rank: 497186
Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting--the Institute of Genetics in Cologne--and is written for newcomers and more experienced researchers in the field. Topics include the Cre/loxP recombination system and gene targeting in ES cells; gene targeting vectors; chromosomal translocations; and identification of homologous recombinants.

Gene Targeting: A Practical Approach (Practical Approach Series)

publisher: Oxford University Press, USA, published: 1993-10-07
ASIN: 0199634068
EAN: 9780199634064
sales rank: 5317973
The book begins by describing in detail the rationale behind designing gene targeting vectors, including both basic replacement vectors for creating null mutations and more sophisticated two-step approaches for creating subtle mutations. It describes the latest techniques for culturing and manipulating ES cells and a number of approaches for identifying rare targeted cell clones. Techniques for culturing and assaying for mouse and human hematopoietic progenitors in vitro and in vivo are then covered, followed by the design of, and use of, retrovirus vectors for introducing and expressing genes in bone marrow cells. Two approaches for making ES cell chimeras are described. One chapter covers the more standard approach of ES cell injection into blastocysts as well as morula injection. Another chapter describes techniques for making aggregation chimeras and in particular a recent approach using tetraploid host embryos to produce completely ES cell-derived embryos and mice. The critical steps for obtaining germline chimeras are discussed. The book is concluded by a discussion of the many uses of enhancer, gene and promoter trap vectors, the advantages of each, and describes in detail the techniques for producing and analyzing "trap" vector insertions in ES cells. The protocols are written with sufficient detail for a novice in the area to use them as a manual to learn the various techniques required for producing mutant mice, beginning with the embryonic stem cells and ending up with chimeric mice for breeding.

Gene Targeting & New Developments in Neurobiology: Proceedings of the 19th International Symposium on Brain Science of the Taniguchi Foundation, Nara, ... (Taniguchi Symposium on Brain Sciences)

publisher: S Karger Pub, published: 1996-11
ASIN: 3805564015
EAN: 9783805564014
This text looks at selected topics in gene targetting and related aspects. These include the mechanisms of hippocampal plasticity responsible for memory and learning, and cerebellar function for motor co-ordination and learning.

Gene Targeting Protocols (Methods in Molecular Biology Ser., Vol. 133)

by: Eric B. (editor) Kmiec
publisher: Humana Press, published: 2000
ASIN: B0041V8XZ8

Cytokine Knockouts (Contemporary Immunology)

publisher: Humana Press, published: 1998-01-15
ASIN: 0896033686
EAN: 9780896033689
sales rank: 3848643
This book is a unique compendium of the latest information on cytokine knockouts. Each chapter-written by the leading scientists who created or studied each knockout-illuminates the physiological consequences of deficiency in a particular cytokine and relates the phenotype to other knockout mice and to human diseases. The effects discussed range from diseases of the bowel, lung, heart, blood, kidney, and liver to immune deficiency, developmental defects, and autoimmunity. Most of the key knockouts of cytokines or receptors, including IL-1 through 8, IFNs, TGFs, TNF, LT, CD40, LIF, CSFs, c-mpl, gp130, and ICE, are thoroughly reviewed.

Gene Therapy

by: Mauro Giacca
publisher: Springer, published: 2010-06-04
ASIN: 8847016428
EAN: 9788847016422
sales rank: 2101018
The overall purpose of this book is to expose the reader/student to a comprehensive and detailed, yet still easily accessible, overview of the whole field of gene therapy, ranging from vector development and therapeutic gene selection to the results of the most recent clinical trials. The language is plain and, whenever possible, non-technical. Since the book is intended to be a textbook in the field of gene therapy in both the basic science and clinical areas, when technical descriptions are required, they are provided either in the main text or in add-on explanatory boxes. For example, clinical readers might find it difficult to understand the principles of viral vector design without knowing some molecular details of viral genome organization and virus life cycle, and basic scientists might be unaware of the clinical and therapeutic advancements for the various disorders treated by the book. In these cases, basic explanations are provided. In this respect, it is worth pointing out that the book is truly a gene "therapy" book; it only considers conditions and experimental approaches based on gene transfer that have already proceeded to the clinic or are close to doing so. Basic research on gene transfer or nucleic acid modification is only presented if it is when close to clinical application. Following the same principle, only diseases for which clinical trials have already been performed or are planned are considered, or those for which solid and encouraging pre-clinical, animal experimentation results are available.

Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, Third Edition

by: Nancy Smyth Templeton
publisher: CRC Press, published: 2008-10-06
ASIN: 084938768X
EAN: 9780849387685
sales rank: 1908462
Since the publication of the second edition of this book in 2004, gene therapy and cell therapy clinical trials have yielded some remarkable successes and some disappointing failures. Now in its third edition, Gene and Cell Therapy: Therapeutic Mechanisms and Strategies assembles many of the new technical advances in gene delivery, clinical applications, and new approaches to the regulation and modification of gene expression.
New Topics Covered in this Edition:
Gene and Cell Therapies for Diabetes and Cardiovascular Diseases

Clinical Trials

Human Embryonic Stem Cells

Tissue Engineering Combined with Cell Therapies

Novel Polymers

Relevant Nanotechnologies

SiRNA Therapeutic Strategies

Dendrimer Technologies

Comprised of contributions from international experts, this book begins with a discussion of delivery systems and therapeutic strategies, exploring retroviral vectors and adenovirus vectors, as well as other therapeutic strategies.
The middle section focuses on gene expression and detection, followed by an examination of various therapeutic strategies for individual diseases, including hematopoietic disorders, cardiovascular conditions, cancer, diabetes, cystic fibrosis, neurological disorders, and childhood-onset blindness.
The final section discusses recent clinical trials and regulatory issues surrounding the new technology.
This compendium is assembled by noted molecular biologist and biochemist Nancy Smyth Templeton. Baylor College of Medicine and several other institutions have used Dr. Templeton’s non-viral therapeutics in clinical trials for the treatment of lung, breast, head and neck, and pancreatic cancers, as well as Hepatitis B and C. She continues to work at the forefront of research in gene and cell therapies. Her contributions, as well as those contained in this volume, are sure to advance the state of the art of these revolutionary life-saving technologies.

Cytokine Gene Polymorphisms in Multifactorial Conditions

publisher: CRC Press, published: 2006-06-21
ASIN: 0849336198
EAN: 9780849336195
sales rank: 5480231
The literature on cytokine genetics is vast, so vast that it is now practically beyond the time or logistical constraints of most scientists to successfully keep pace with it. A compilation of the latest research, Cytokine Gene Polymorphisms in Multifactorial Conditions brings together, reviews, and structures up-to-date information on polymorphisms in cytokine genes. It discusses haplotype structures and linkage disequilibrium patterns in cytokine gene loci; functional biological effects of polymorphisms; and genetic associations with disease. The book documents polymorphisms in the most important cytokine genes, or gene clusters, and their biological and genetic effects in a multitude of distinct multifactorial conditions.

Unique to this book are the "disease-centered" chapters examining the role of cytokine gene polymorphisms in a multitude of multifactorial conditions. The conditions include autoimmune or chronic inflammatory diseases, cardiovascular disease, infectious diseases, and longevity. "This section is a real tour de force" (Grant Gallagher and Michael F. Seldin, March 2006).

Broadening the understanding of the effect of genetic variations on human immune responses, the organization, scope, and content of this book make it a valuable and easily accessible resource. The book integrates genetic, immunological, and clinical information and will serve as a reference for novice and expert geneticists, immunologists, cell biologists and clinicians. It is a must for everyone involved in, or planning, cytokine genetics or immunogenetics studies.

Handbook of Biodegradable Polymers (Drug Targeting and Delivery)

publisher: CRC Press, published: 1998-02-04
ASIN: 9057021536
EAN: 9789057021534
sales rank: 1234183
Handbook of Biodegradable Polymers, the seventh volume in the Drug Delivery and Targeting book series, provides a source manual for synthetic procedures, properties and applications of bioerodible polymers. The authors describe widely available materials such as polyactides, collagen and gelatin, as well as polymers of emerging importance, such as the genetically-engineered and elastin-based polymers which are either proprietary or in early stages of development. Section I addresses synthetic absorbable polymers, and Section 2 profiles natural, semi-synthetic and biosynthetic polymers. Section 3 discusses the surface characterization of degradable polymers, the modeling of biodegradation and non-medical polymers. This book is ideal for researchers from academia and industry as well as chemists, pharmacists and physicians who deal with biopolymers, drug delivery and targeting, bioengineering and implantable devices.

Cardiac Gene Expression: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2007-03-19
ASIN: 1588293521
EAN: 9781588293527
sales rank: 1505989
This book presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. The concluding section of the book highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.

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	Laboratory Protocols for Conditional Gene Targeting by: Raul M. Torres publisher: Oxford University Press, USA, published: 1997-10-02 ASIN: 019963677X EAN: 9780199636778 sales rank: 497186 Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting--the Institute of Genetics in Cologne--and is written for newcomers and more experienced researchers in the field. Topics include the Cre/loxP recombination system and gene targeting in ES cells; gene targeting vectors; chromosomal translocations; and identification of homologous recombinants.
	Gene Targeting: A Practical Approach (Practical Approach Series) publisher: Oxford University Press, USA, published: 1993-10-07 ASIN: 0199634068 EAN: 9780199634064 sales rank: 5317973 The book begins by describing in detail the rationale behind designing gene targeting vectors, including both basic replacement vectors for creating null mutations and more sophisticated two-step approaches for creating subtle mutations. It describes the latest techniques for culturing and manipulating ES cells and a number of approaches for identifying rare targeted cell clones. Techniques for culturing and assaying for mouse and human hematopoietic progenitors in vitro and in vivo are then covered, followed by the design of, and use of, retrovirus vectors for introducing and expressing genes in bone marrow cells. Two approaches for making ES cell chimeras are described. One chapter covers the more standard approach of ES cell injection into blastocysts as well as morula injection. Another chapter describes techniques for making aggregation chimeras and in particular a recent approach using tetraploid host embryos to produce completely ES cell-derived embryos and mice. The critical steps for obtaining germline chimeras are discussed. The book is concluded by a discussion of the many uses of enhancer, gene and promoter trap vectors, the advantages of each, and describes in detail the techniques for producing and analyzing "trap" vector insertions in ES cells. The protocols are written with sufficient detail for a novice in the area to use them as a manual to learn the various techniques required for producing mutant mice, beginning with the embryonic stem cells and ending up with chimeric mice for breeding.
	Gene Targeting & New Developments in Neurobiology: Proceedings of the 19th International Symposium on Brain Science of the Taniguchi Foundation, Nara, ... (Taniguchi Symposium on Brain Sciences) publisher: S Karger Pub, published: 1996-11 ASIN: 3805564015 EAN: 9783805564014 This text looks at selected topics in gene targetting and related aspects. These include the mechanisms of hippocampal plasticity responsible for memory and learning, and cerebellar function for motor co-ordination and learning.
	Gene Targeting Protocols (Methods in Molecular Biology Ser., Vol. 133) by: Eric B. (editor) Kmiec publisher: Humana Press, published: 2000 ASIN: B0041V8XZ8
	Cytokine Knockouts (Contemporary Immunology) publisher: Humana Press, published: 1998-01-15 ASIN: 0896033686 EAN: 9780896033689 sales rank: 3848643 This book is a unique compendium of the latest information on cytokine knockouts. Each chapter-written by the leading scientists who created or studied each knockout-illuminates the physiological consequences of deficiency in a particular cytokine and relates the phenotype to other knockout mice and to human diseases. The effects discussed range from diseases of the bowel, lung, heart, blood, kidney, and liver to immune deficiency, developmental defects, and autoimmunity. Most of the key knockouts of cytokines or receptors, including IL-1 through 8, IFNs, TGFs, TNF, LT, CD40, LIF, CSFs, c-mpl, gp130, and ICE, are thoroughly reviewed.
	Gene Therapy by: Mauro Giacca publisher: Springer, published: 2010-06-04 ASIN: 8847016428 EAN: 9788847016422 sales rank: 2101018 The overall purpose of this book is to expose the reader/student to a comprehensive and detailed, yet still easily accessible, overview of the whole field of gene therapy, ranging from vector development and therapeutic gene selection to the results of the most recent clinical trials. The language is plain and, whenever possible, non-technical. Since the book is intended to be a textbook in the field of gene therapy in both the basic science and clinical areas, when technical descriptions are required, they are provided either in the main text or in add-on explanatory boxes. For example, clinical readers might find it difficult to understand the principles of viral vector design without knowing some molecular details of viral genome organization and virus life cycle, and basic scientists might be unaware of the clinical and therapeutic advancements for the various disorders treated by the book. In these cases, basic explanations are provided. In this respect, it is worth pointing out that the book is truly a gene "therapy" book; it only considers conditions and experimental approaches based on gene transfer that have already proceeded to the clinic or are close to doing so. Basic research on gene transfer or nucleic acid modification is only presented if it is when close to clinical application. Following the same principle, only diseases for which clinical trials have already been performed or are planned are considered, or those for which solid and encouraging pre-clinical, animal experimentation results are available.
	Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, Third Edition by: Nancy Smyth Templeton publisher: CRC Press, published: 2008-10-06 ASIN: 084938768X EAN: 9780849387685 sales rank: 1908462 Since the publication of the second edition of this book in 2004, gene therapy and cell therapy clinical trials have yielded some remarkable successes and some disappointing failures. Now in its third edition, Gene and Cell Therapy: Therapeutic Mechanisms and Strategies assembles many of the new technical advances in gene delivery, clinical applications, and new approaches to the regulation and modification of gene expression. New Topics Covered in this Edition: Gene and Cell Therapies for Diabetes and Cardiovascular Diseases Clinical Trials Human Embryonic Stem Cells Tissue Engineering Combined with Cell Therapies Novel Polymers Relevant Nanotechnologies SiRNA Therapeutic Strategies Dendrimer Technologies Comprised of contributions from international experts, this book begins with a discussion of delivery systems and therapeutic strategies, exploring retroviral vectors and adenovirus vectors, as well as other therapeutic strategies. The middle section focuses on gene expression and detection, followed by an examination of various therapeutic strategies for individual diseases, including hematopoietic disorders, cardiovascular conditions, cancer, diabetes, cystic fibrosis, neurological disorders, and childhood-onset blindness. The final section discusses recent clinical trials and regulatory issues surrounding the new technology. This compendium is assembled by noted molecular biologist and biochemist Nancy Smyth Templeton. Baylor College of Medicine and several other institutions have used Dr. Templeton’s non-viral therapeutics in clinical trials for the treatment of lung, breast, head and neck, and pancreatic cancers, as well as Hepatitis B and C. She continues to work at the forefront of research in gene and cell therapies. Her contributions, as well as those contained in this volume, are sure to advance the state of the art of these revolutionary life-saving technologies.
	Cytokine Gene Polymorphisms in Multifactorial Conditions publisher: CRC Press, published: 2006-06-21 ASIN: 0849336198 EAN: 9780849336195 sales rank: 5480231 The literature on cytokine genetics is vast, so vast that it is now practically beyond the time or logistical constraints of most scientists to successfully keep pace with it. A compilation of the latest research, Cytokine Gene Polymorphisms in Multifactorial Conditions brings together, reviews, and structures up-to-date information on polymorphisms in cytokine genes. It discusses haplotype structures and linkage disequilibrium patterns in cytokine gene loci; functional biological effects of polymorphisms; and genetic associations with disease. The book documents polymorphisms in the most important cytokine genes, or gene clusters, and their biological and genetic effects in a multitude of distinct multifactorial conditions. Unique to this book are the "disease-centered" chapters examining the role of cytokine gene polymorphisms in a multitude of multifactorial conditions. The conditions include autoimmune or chronic inflammatory diseases, cardiovascular disease, infectious diseases, and longevity. "This section is a real tour de force" (Grant Gallagher and Michael F. Seldin, March 2006). Broadening the understanding of the effect of genetic variations on human immune responses, the organization, scope, and content of this book make it a valuable and easily accessible resource. The book integrates genetic, immunological, and clinical information and will serve as a reference for novice and expert geneticists, immunologists, cell biologists and clinicians. It is a must for everyone involved in, or planning, cytokine genetics or immunogenetics studies.
	Handbook of Biodegradable Polymers (Drug Targeting and Delivery) publisher: CRC Press, published: 1998-02-04 ASIN: 9057021536 EAN: 9789057021534 sales rank: 1234183 Handbook of Biodegradable Polymers, the seventh volume in the Drug Delivery and Targeting book series, provides a source manual for synthetic procedures, properties and applications of bioerodible polymers. The authors describe widely available materials such as polyactides, collagen and gelatin, as well as polymers of emerging importance, such as the genetically-engineered and elastin-based polymers which are either proprietary or in early stages of development. Section I addresses synthetic absorbable polymers, and Section 2 profiles natural, semi-synthetic and biosynthetic polymers. Section 3 discusses the surface characterization of degradable polymers, the modeling of biodegradation and non-medical polymers. This book is ideal for researchers from academia and industry as well as chemists, pharmacists and physicians who deal with biopolymers, drug delivery and targeting, bioengineering and implantable devices.
	Cardiac Gene Expression: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2007-03-19 ASIN: 1588293521 EAN: 9781588293527 sales rank: 1505989 This book presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. The concluding section of the book highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.