Related books on 'hplc'
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by: Marvin McMaster publisher: Wiley-Interscience, published: 2006-12-18 ASIN: 0471754013 EAN: 9780471754015 sales rank: 1371883 This Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Complete with a CD-ROM and appendices, this guide has everything chromatographers need to know to confidently separate, identify, purify, and quantify compounds. Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file. |
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publisher: CRC Press, published: 2010-07-14 ASIN: 1574445545 EAN: 9781574445541 sales rank: 1799396 High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the publication of the benchmark first edition twelve years ago. It reports detailed information on fundamental and practical aspects of HPLC related to conventional format and sophisticated novel approaches which have been developed to address a variety of separation problems in different fields. The latest concepts New concepts presented in this edition include monolithic columns, bonded stationary phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, and capillary electromigration techniques. The book also discusses LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, field-flow fractionation, retention models for ions, and polymer HPLC. Fundamentals and applications The first section of the book explores emerging novel aspects of HPLC and related separation methods based on the differential velocity of analytes in a liquid medium under the action of either an electric field (capillary electromigration techniques) or a gravitational field (field-flow fractionation). The section focusing on applications highlights four significant areas in which HPLC is successfully employed: chiral pharmaceutical, environmental analysis, food analysis, and forensic science. Ideal for a widespread audience Explanatory figures and tables supplement the text and clarify difficult concepts. Extensive references provide gateways to more focused study. Suitable for undergraduates or new practitioners interested in improving their knowledge on the current status and future trends of HPLC, the book is also a critical resource for researchers looking for solutions to complex separation problems or those who currently use HPLC either as an analytical or a preparative scale tool. |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617372862 EAN: 9781617372865 sales rank: 1777006 Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology. |
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by: Paul C. Sadek publisher: Wiley-Interscience, published: 1999-10-05 ASIN: 0471178349 EAN: 9780471178347 sales rank: 2330381 A complete, up-to-date guide to the use, maintenance, and troubleshooting of HPLC systems The last twenty-five years have seen a dramatic rise in the use of High Performance Liquid Chromatography (HPLC) in laboratories worldwide. Troubleshooting HPLC Systems provides analysts as well as laboratory technicians and managers with a readily accessible and immensely useful guide to the new generation of HPLC equipment and techniques. With an emphasis on effective troubleshooting of HPLC systems, this lab companion covers system configuration and functions, problem-solving procedures, maintenance, and HPLC basics. It then walks chromatographers investigating the source of a malfunction through each system component-from solvents and reservoirs to sample preparation to columns and detectors. Special features of Troubleshooting HPLC Systems include: * A detailed review of HPLC instrumentation and accessories * The role of operating parameters as indicators of system performance * Step-by-step troubleshooting protocols for each system component * How to set up a preventive maintenance program for HPLC systems * An overview of the categories of HPLC separations * A compilation of HPLC terms and definitions * Tables and charts detailing solvents' properties |
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publisher: John Wiley & Sons, published: 1989-11-10 ASIN: 0471562319 EAN: 9780471562313 sales rank: 7795681 Offers a concise background in HPLC methodology and includes illustrative examples of some of the key advances made in the receptor field through application of this chromatographic technique. A brief historical overview puts into perspective the specific state of the art HPLC laboratory applications that are contained in the book. Technical contributions discuss how best to select the HPLC mode for a particular biochemical problem, including the most effective use of a particularly sensitive technique, microbore HPLC for synthesis, purification, and characterization of synthetic peptides. |
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publisher: Academic Press, published: 1992-11-03 ASIN: 0125456808 EAN: 9780125456807 sales rank: 1749778 This guide for the practicing chromatographer who wants a ready source of information on HPLC detection explores and compares existing detection systems and detectors, outlines the common problems associated with a given detector, and offers proven approaches to avoiding such problems. Key Features * Addresses the practical aspects of HPLC detection, including: basic theory * when a particular type of detector can be used * how detectors from various manufacturers differ * common problems of detectors and ways to avoid them * Presents an overview of today's most common techniques * Discusses the advantages and disadvantages of HPLC, dispelling common misconceptions |
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by: Hermann Mascher publisher: Wiley-VCH, published: 2012-02-21 ASIN: 3527331298 EAN: 9783527331291 sales rank: 1964868 Filling a gap in the literature for a hands-on guide focusing on everyday laboratory challenges, this English edition has been expanded and revised using the feedback received on the successful German precursor. Throughout the book, Professor Mascher draws on his 30 years of experience and provides abundant practical advice, troubleshooting and other hints highlighted in boxes, as well as a broad selection of walk-through case studies. Based on a course taught by the author, the first part of the book intuitively explains all steps of a routine pharmaceutical analysis and explains how to set up a robust, inexpensive and efficient method for a given substance. In the second part he includes 20 worked example cases that highlight common challenges and how to overcome them. With its appendix containing tried-and-tested analytical methods for 100 clinically relevant substances from the author's own laboratory, complete with spectral and MS data as well as literature references, this is a life-long companion for everyone working in clinical, pharmaceutical and biochemical analysis. |
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by: George Lunn publisher: Wiley-Interscience, published: 2005-04-18 ASIN: 0471669415 EAN: 9780471669418 sales rank: 2833938 An indispensable resource for busy researchers Your time is valuable-too valuable to spend hunting through the technical literature in search of the right HPLC assay techniques for your projects. With HPLC Methods for Recently Approved Pharmaceuticals, you'll quickly identify and replicate the ideal procedures for your project needs, without having to refer to original source publications. More of your time can then be spent in the lab, not the library. Covering the relevant world literature through 2003, this book picks up where Dr. Lunn's acclaimed HPLC Methods for Pharmaceutical Analysis left off. It arms you with established HPLC assay techniques for hundreds of newly approved drugs, as well as drugs for which assay methods were only recently developed. Combining detailed descriptions of procedures with specially annotated references, this practical handbook gives you: * HPLC methods for 390 commonly prescribed pharmaceutical compounds * Various procedures for each drug listed together-making it easy to mix and match for customized approaches * Methods for drugs in biological fluids and for bulk and formulated drugs * Chemical structures, molecular weights and formulas, and CAS Registry Numbers * Cross-references to The Merck Index * Retention times of other drugs that can be assayed using the same methods |
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by: Stavros Kromidas publisher: Wiley-VCH, published: 2000-05-19 ASIN: 3527298428 EAN: 9783527298426 sales rank: 2210482 Quick and easy solutions to HPLC problems! For 45 typical questions of HPLC users, the answer and general conclusions are presented on no more than 4 pages each. The book covers - simple equipment tests and selection criteria for columns, buffers etc. - specific problems and how to solve them - options for optimizing separations. The book also features a special chapter on the retention of ionizable components in RP-HPLC, references, data tables and check lists. It's a first-aid kit for every HPLC user. Stavros Kromidas is managing director of NOVIA, a company offering consulting services and training courses for analytical laboratories. For five years he was sales manager for the HPLC manufacturer Waters in Germany. He is author and editor of numerous books. |
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publisher: Wiley-VCH, published: 2006-07-31 ASIN: 352731377X EAN: 9783527313778 sales rank: 2412024 The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports. |