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Transmembrane Signaling Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617376337
EAN: 9781617376337
sales rank: 5393158
This fully updated and expanded second edition of Transmembrane Signaling Protocols demonstrates the use of such techniques as single cell imaging, bioluminecence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Cellular and Molecular Immunology: with STUDENT CONSULT Online Access, 7e (Abbas, Cellular and Molecular Immunology)

by: Abul K. Abbas MBBS
publisher: Saunders, published: 2011-05-09
ASIN: 1437715281
EAN: 9781437715286
sales rank: 24954

Cellular and Molecular Immunology takes a comprehensive yet straightforward approach to the latest developments in this active and fast-changing field. Drs. Abul K. Abbas, Andrew H. Lichtman, and Shiv Pillai present sweeping updates in this new edition to cover antigen receptors and signal transduction in immune cells, mucosal and skin immunity, cytokines, leukocyte-endothelial interaction, and more. In print and online with additional resources, this reference is the up-to-date and readable textbook you need to master the complex subject of immunology.
Access to www.studentconsult.com and Evolve provides informative lecture slides based on images in the book, fully modified for incorporation into your lesson plans. Other online features include searchable text, an image bank, self-assessment questions, and animations.

Recognize the clinical relevance of the immunology through discussions of the implications of immunologic science for the management of human disease.

Grasp the details of experimental observations that form the basis for the science of immunology at the molecular, cellular, and whole-organism levels and draw the appropriate conclusions.

Stay abreast of the latest advances in immunology and molecular biology through extensive updates that cover cytokines, innate immunity, leukocyte-endothelial interactions, signaling, costimulation, and more.

Visualize immunologic processes more effectively through a completely revised art program with redrawn figures, a brighter color palette, and more 3-dimensional art.

Find information more quickly and easily through a reorganized chapter structure and a more logical flow of material.

Therapeutic Antibodies: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2009-03-05
ASIN: 1934115924
EAN: 9781934115923
sales rank: 1374095
With revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sections devoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Therapeutic Antibodies: Methods and Protocols serves as a key resource for researchers and antibody engineers investigating and participating in this rapidly growing therapeutic market segment.

Vaccinia Virus and Poxvirology: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617374377
EAN: 9781617374371
sales rank: 2610142
A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.

Ion Channels: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-12-09
ASIN: 1617376523
EAN: 9781617376528
The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.

Embryonic Stem Cell Protocols: Volume I: Isolation and Characterization (Methods in Molecular Biology)

publisher: Humana Press, published: 2006-02-15
ASIN: 1588294986
EAN: 9781588294982
sales rank: 1837149
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Embryonic Stem Cell Protocols: Volume II: Differentiation Models (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617377775
EAN: 9781617377778
sales rank: 4399756
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

mTOR: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2011-12-02
ASIN: 1617794295
EAN: 9781617794292
sales rank: 1017994
Since its discovery, the mammalian target of rapamycin (mTOR) has been shown to regulate many critical molecular processes in eukaryotes such as metabolism, growth, survival, aging, synaptic plasticity, memory, and immunity. In mTOR: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study mTOR. These include methods and techniques used for the study of the mTOR pathway and potential therapeutic applications of mTOR inhibitors such as, immunosuppressive and anticancer agents. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, mTOR: Methods and Protocols seeks to aid scientists in the further study of this crucially important molecule ranging from the analysis of signal transduction events within a cell to the assessment of complex human diseases such as metabolic disorders or cancer.

Microchip Capillary Electrophoresis: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617374768
EAN: 9781617374760
Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

PRINS and In Situ PCR Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-12-16
ASIN: 1617376361
EAN: 9781617376368
This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.

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	Transmembrane Signaling Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617376337 EAN: 9781617376337 sales rank: 5393158 This fully updated and expanded second edition of Transmembrane Signaling Protocols demonstrates the use of such techniques as single cell imaging, bioluminecence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
	Cellular and Molecular Immunology: with STUDENT CONSULT Online Access, 7e (Abbas, Cellular and Molecular Immunology) by: Abul K. Abbas MBBS publisher: Saunders, published: 2011-05-09 ASIN: 1437715281 EAN: 9781437715286 sales rank: 24954 Cellular and Molecular Immunology takes a comprehensive yet straightforward approach to the latest developments in this active and fast-changing field. Drs. Abul K. Abbas, Andrew H. Lichtman, and Shiv Pillai present sweeping updates in this new edition to cover antigen receptors and signal transduction in immune cells, mucosal and skin immunity, cytokines, leukocyte-endothelial interaction, and more. In print and online with additional resources, this reference is the up-to-date and readable textbook you need to master the complex subject of immunology. Access to www.studentconsult.com and Evolve provides informative lecture slides based on images in the book, fully modified for incorporation into your lesson plans. Other online features include searchable text, an image bank, self-assessment questions, and animations. Recognize the clinical relevance of the immunology through discussions of the implications of immunologic science for the management of human disease. Grasp the details of experimental observations that form the basis for the science of immunology at the molecular, cellular, and whole-organism levels and draw the appropriate conclusions. Stay abreast of the latest advances in immunology and molecular biology through extensive updates that cover cytokines, innate immunity, leukocyte-endothelial interactions, signaling, costimulation, and more. Visualize immunologic processes more effectively through a completely revised art program with redrawn figures, a brighter color palette, and more 3-dimensional art. Find information more quickly and easily through a reorganized chapter structure and a more logical flow of material.
	Therapeutic Antibodies: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2009-03-05 ASIN: 1934115924 EAN: 9781934115923 sales rank: 1374095 With revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sections devoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Therapeutic Antibodies: Methods and Protocols serves as a key resource for researchers and antibody engineers investigating and participating in this rapidly growing therapeutic market segment.
	Vaccinia Virus and Poxvirology: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617374377 EAN: 9781617374371 sales rank: 2610142 A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.
	Ion Channels: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-12-09 ASIN: 1617376523 EAN: 9781617376528 The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.
	Embryonic Stem Cell Protocols: Volume I: Isolation and Characterization (Methods in Molecular Biology) publisher: Humana Press, published: 2006-02-15 ASIN: 1588294986 EAN: 9781588294982 sales rank: 1837149 Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
	Embryonic Stem Cell Protocols: Volume II: Differentiation Models (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617377775 EAN: 9781617377778 sales rank: 4399756 Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
	mTOR: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2011-12-02 ASIN: 1617794295 EAN: 9781617794292 sales rank: 1017994 Since its discovery, the mammalian target of rapamycin (mTOR) has been shown to regulate many critical molecular processes in eukaryotes such as metabolism, growth, survival, aging, synaptic plasticity, memory, and immunity. In mTOR: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study mTOR. These include methods and techniques used for the study of the mTOR pathway and potential therapeutic applications of mTOR inhibitors such as, immunosuppressive and anticancer agents. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, mTOR: Methods and Protocols seeks to aid scientists in the further study of this crucially important molecule ranging from the analysis of signal transduction events within a cell to the assessment of complex human diseases such as metabolic disorders or cancer.
	Microchip Capillary Electrophoresis: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617374768 EAN: 9781617374760 Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.
	PRINS and In Situ PCR Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-12-16 ASIN: 1617376361 EAN: 9781617376368 This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.