Related books on 'immunology-protocol'
Return to life science book listNumber of products: 461
Page 2 of 47
|
publisher: Humana Press, published: 1999-03-15 ASIN: 0896033694 EAN: 9780896033696 sales rank: 3403067 This collection of cutting-edge techniques is essential for studying the molecular biology, virology, and immunology of the HIV virus. The methods cover a broad range of research interests, including quantitation of viral genomes, HIV promoter function, B-cell epitope mapping, viral coreceptor usage, and measurements of T-cell function. Special emphasis is accorded to studying those viral and host immune responses to infection that will be critical to the design of effective preventive vaccines. HIV Protocols is the first HIV methods book to concentrate equally on virology, molecular biology, and immunology, as well as to incorporate methods on chemokine receptor structure and function. |
|
publisher: Humana Press, published: 2010-11-10 ASIN: 1617371378 EAN: 9781617371370 sales rank: 4515630 Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer. |
|
publisher: Humana Press, published: 2010-11-10 ASIN: 1617371513 EAN: 9781617371516 sales rank: 5792626 Expert investigators describe in detail the most widely used techniques in chemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins. |
|
publisher: Humana Press, published: 2010-11-10 ASIN: 1617371661 EAN: 9781617371660 Well-recognized and innovative experimentalists detail their cutting-edge methods for studying the antigen processing and presentation. Drawing on expertise from biochemistry, cell biology, and immunology, they describe step-by-step methods designed to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to the MHC/peptide complex. Emphasis is given those technical steps critical for experimental success that are often omitted from methods published in the primary literature. Eminently accessible and state-of-the-art, Antigen Processing and Presentation Protocols provides both new and experienced investigators with highly practical tools that will extend the questions that can be asked, and effectively be answered, concerning antigen processing/presentation. |
|
publisher: Humana Press, published: 2010-11-09 ASIN: 1617371629 EAN: 9781617371622 Luke O'Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics. |
|
publisher: Humana Press, published: 2010-11-10 ASIN: 1617373184 EAN: 9781617373183 Hands-on laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Although the protocols require little previous experience, even highly skilled researchers will augment their skills with many novel timesaving techniques. |
|
publisher: Humana Press, published: 2010-11-19 ASIN: 1617378089 EAN: 9781617378089 sales rank: 4869823 Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors. In "HIV Protocols, Second Edition", expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, "HIV Protocols, Second Edition" is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1. |
|
publisher: Humana Press, published: 2002-10-23 ASIN: 0896039846 EAN: 9780896039841 sales rank: 5096520 Leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of superantigens. Their protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level. |
|
publisher: Wiley-Blackwell, published: 1998-09-15 ASIN: 0865425183 EAN: 9780865425187 sales rank: 3986371 This is the most comprehensive, up-to-date and one-volume guide to protocols in the immunology lab available anywhere. Carefully edited by two of the leading clinical and laboratory immunologists in the world, with concise chapters by 69 experts in their respective subspecialties, this book serves as both a useful reference and a practical manual of laboratory protocols. Published under the auspices of the Americal Medical Laboratory Immunologists, Clinical Diagnosis Immunology is designed to be useful in the day-to-day work of all medical laboratory professionals. It is an indispensable new tool for the modern medical lab, destined to become the standard reference/text in the field. |
|
by: Frank C. Hay publisher: Wiley-Blackwell, published: 2002-01-28 ASIN: 0865429618 EAN: 9780865429611 sales rank: 1692804 Practical Immunology is a basic text aimed at immunology students and researchers at all levels who need a comprehensive overview of the methodology of immunology. The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice and it has always been the aim of this book to educate researchers in the design and performance of complex techniques. It will appeal to students of immunlogy, graduate students embarking on bench science, or specialised immunologists who need to use an immunological technique outside their sphere of expertise.
|