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Fever often absent in early enteroviral illness. (First Survey in Using PCR).(reverse-transcriptase polymerase chain reaction): An article from: Family Practice News

by: Miriam E. Tucker
publisher: International Medical News Group, published: 2003-01-15
ASIN: B0008G7BK8
This digital document is an article from Family Practice News, published by International Medical News Group on January 15, 2003. The length of the article is 724 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

Citation Details
Title: Fever often absent in early enteroviral illness. (First Survey in Using PCR).(reverse-transcriptase polymerase chain reaction)
Author: Miriam E. Tucker
Publication: Family Practice News (Magazine/Journal)
Date: January 15, 2003
Publisher: International Medical News Group
Volume: 33 Issue: 2 Page: 50(1)

Distributed by Thomson Gale

Rapid Cycle Real-Time PCR-Methods and Applications: Quantification

publisher: Springer, published: 2004-02-20
ASIN: B000QFALZ8
sales rank: 884080

Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!

Biotechnology: Proteins to PCR: A Course in Strategies and Lab Techniques

by: David Burden
publisher: Birkhäuser Boston, published: 1995-11-01
ASIN: 0817637567
EAN: 9780817637569
sales rank: 4494061
This is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis. Laboratory topics include: microbiological methods (handling, storage, media); organism growth and protein production; enzyme and protein essays (specific activity); batch purification of proteins; gel filtration and ion exchange chromatographies; genomic and plasmid DNA isolation; and DNA sequencing and computer analysis.

RT-PCR Protocols: Second Edition (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-03-22
ASIN: 1607616289
EAN: 9781607616283
sales rank: 2301085
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

Molecular Diagnostic PCR Handbook

publisher: Springer, published: 2010-12-08
ASIN: 9048168562
EAN: 9789048168569
sales rank: 3132895
This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented. The difficulties, advantages and disadvantages in PCR applications are explained and placed in context with other test systems. Emphasis is placed on the use of PCR for detection of pathogens with a particular focus on diagnosticians and scientists from the developing world. It is hoped that this book will enable readers from various disciplines and levels of expertise to better judge the merits of PCR and to increase their skills and knowledge in order to assist in a more logical, efficient and assured use of this technology.

Real-Time PCR in Microbiology: From Diagnosis to Characterisation

publisher: Caister Academic Press, published: 2007-07-01
ASIN: 1904455182
EAN: 9781904455189
sales rank: 1823304
Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR by presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardization, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion, providing an insightful, topical, and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. The editor and authors have produced an excellent book that will be extremely useful for all microbiologists.

PCR Strategies

publisher: Academic Press, published: 1995-06-27
ASIN: 0123991668
EAN: 9780123991669
PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both!

Real-Time PCR: Current Technology and Applications

publisher: Caister Academic Press, published: 2009-01-01
ASIN: 1904455395
EAN: 9781904455394
sales rank: 1241759
Real-time PCR (RT-PCR) technology is highly flexible and many alternative instruments and fluorescent probe systems have been developed recently. The decreased hands-on time, increased reliability, and improved quantitative accuracy of RT-PCR methods have contributed to the adoption of RT-PCR for a wide range of new applications. This essential manual presents a comprehensive guide to the most up-to-date technologies and applications, as well as providing an overview of the theory of this increasingly important technique. Renowned experts in the field describe and discuss the latest PCR platforms, fluorescent chemistries, validation software, data analysis, and internal and external controls. This timely and authoritative volume also discusses a wide range of RT-PCR applications including clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping, and much more. Real-Time PCR: Current Technology and Applications will be an essential book for all laboratories using PCR.

PCR Protocols: Current Methods and Applications (Methods in Molecular Biology)

publisher: Humana Press, published: 1993-01-01
ASIN: 0896032442
EAN: 9780896032446
sales rank: 1879488
The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols.

Key Topics and Techniques: basic startup procedures for PCR • PCR in detecting DNA and RNA • PCR in DNA synthesis and mutagenesis • PCR cloning of DNA • selection of primers • radioactive and nonradioactive labeling • quantitation of PCR products by HPLC • chromosome assignment • PCR mapping of HLA genes • sequencing of PCR products • genomic footprinting • quantitation of tumor gene expression • screening of phage libraries • SSP-PCR and genome walking • subcloning of PCR products.

PCR Protocols (Methods in Molecular Biology) (Methods in Molecular Biology) (Methods in Molecular Biology)

publisher: Humana Press, published: 2003-09-01
ASIN: 0896036421
EAN: 9780896036420
sales rank: 2193728
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

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	Fever often absent in early enteroviral illness. (First Survey in Using PCR).(reverse-transcriptase polymerase chain reaction): An article from: Family Practice News by: Miriam E. Tucker publisher: International Medical News Group, published: 2003-01-15 ASIN: B0008G7BK8 This digital document is an article from Family Practice News, published by International Medical News Group on January 15, 2003. The length of the article is 724 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser. Citation Details Title: Fever often absent in early enteroviral illness. (First Survey in Using PCR).(reverse-transcriptase polymerase chain reaction) Author: Miriam E. Tucker Publication: Family Practice News (Magazine/Journal) Date: January 15, 2003 Publisher: International Medical News Group Volume: 33 Issue: 2 Page: 50(1) Distributed by Thomson Gale
	Rapid Cycle Real-Time PCR-Methods and Applications: Quantification publisher: Springer, published: 2004-02-20 ASIN: B000QFALZ8 sales rank: 884080 Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
	Biotechnology: Proteins to PCR: A Course in Strategies and Lab Techniques by: David Burden publisher: Birkhäuser Boston, published: 1995-11-01 ASIN: 0817637567 EAN: 9780817637569 sales rank: 4494061 This is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis. Laboratory topics include: microbiological methods (handling, storage, media); organism growth and protein production; enzyme and protein essays (specific activity); batch purification of proteins; gel filtration and ion exchange chromatographies; genomic and plasmid DNA isolation; and DNA sequencing and computer analysis.
	RT-PCR Protocols: Second Edition (Methods in Molecular Biology) publisher: Humana Press, published: 2010-03-22 ASIN: 1607616289 EAN: 9781607616283 sales rank: 2301085 Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
	Molecular Diagnostic PCR Handbook publisher: Springer, published: 2010-12-08 ASIN: 9048168562 EAN: 9789048168569 sales rank: 3132895 This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented. The difficulties, advantages and disadvantages in PCR applications are explained and placed in context with other test systems. Emphasis is placed on the use of PCR for detection of pathogens with a particular focus on diagnosticians and scientists from the developing world. It is hoped that this book will enable readers from various disciplines and levels of expertise to better judge the merits of PCR and to increase their skills and knowledge in order to assist in a more logical, efficient and assured use of this technology.
	Real-Time PCR in Microbiology: From Diagnosis to Characterisation publisher: Caister Academic Press, published: 2007-07-01 ASIN: 1904455182 EAN: 9781904455189 sales rank: 1823304 Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR by presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardization, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion, providing an insightful, topical, and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. The editor and authors have produced an excellent book that will be extremely useful for all microbiologists.
	PCR Strategies publisher: Academic Press, published: 1995-06-27 ASIN: 0123991668 EAN: 9780123991669 PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both!
	Real-Time PCR: Current Technology and Applications publisher: Caister Academic Press, published: 2009-01-01 ASIN: 1904455395 EAN: 9781904455394 sales rank: 1241759 Real-time PCR (RT-PCR) technology is highly flexible and many alternative instruments and fluorescent probe systems have been developed recently. The decreased hands-on time, increased reliability, and improved quantitative accuracy of RT-PCR methods have contributed to the adoption of RT-PCR for a wide range of new applications. This essential manual presents a comprehensive guide to the most up-to-date technologies and applications, as well as providing an overview of the theory of this increasingly important technique. Renowned experts in the field describe and discuss the latest PCR platforms, fluorescent chemistries, validation software, data analysis, and internal and external controls. This timely and authoritative volume also discusses a wide range of RT-PCR applications including clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping, and much more. Real-Time PCR: Current Technology and Applications will be an essential book for all laboratories using PCR.
	PCR Protocols: Current Methods and Applications (Methods in Molecular Biology) publisher: Humana Press, published: 1993-01-01 ASIN: 0896032442 EAN: 9780896032446 sales rank: 1879488 The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR • PCR in detecting DNA and RNA • PCR in DNA synthesis and mutagenesis • PCR cloning of DNA • selection of primers • radioactive and nonradioactive labeling • quantitation of PCR products by HPLC • chromosome assignment • PCR mapping of HLA genes • sequencing of PCR products • genomic footprinting • quantitation of tumor gene expression • screening of phage libraries • SSP-PCR and genome walking • subcloning of PCR products.
	PCR Protocols (Methods in Molecular Biology) (Methods in Molecular Biology) (Methods in Molecular Biology) publisher: Humana Press, published: 2003-09-01 ASIN: 0896036421 EAN: 9780896036420 sales rank: 2193728 In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.