Related books on 'protein-purification'
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publisher: Academic Press, published: 1993-06-03 ASIN: 0124802826 EAN: 9780124802827 sales rank: 260636 Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Key Features Highlights of the Second Edition: * Mass spectrophotometric characterization of proteins and peptides * N-terminal sequencing of proteins with blocked N termini * Internal amino acid sequence analysis after protease digestion in-gel and on-blot * Improved microscale peptide purification methods * Computer analysis of protein sequences * New protocols tested and refined through everyday use in authors' laboratories * Updated reference chapter covering all aspects of protein microsequencing |
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by: Ian M. Rosenberg publisher: Birkhäuser Boston, published: 2004-10-21 ASIN: 0817643400 EAN: 9780817643409 sales rank: 1213120 This comprehensive, practical, and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. It will also appeal to experienced researches and graduate students as a benchtop handbook which, without sacrificing scientific concepts, maintains the spirit of experimentation. |
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by: Hafiz Ahmed publisher: CRC Press, published: 2007-04-16 ASIN: B000Q35YXE sales rank: 869437 No description available |
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publisher: Wiley, published: 2012-01-24 ASIN: 0470497181 EAN: 9780470497180 sales rank: 430452 This book describes how to address the analysis of aggregates and particles in protein pharmaceuticals, provides a comprehensive overview of current methods and integrated approaches used to quantify and characterize aggregates and particles, and discusses regulatory requirements. Analytical methods covered in the book include separation, light scattering, microscopy, and spectroscopy. |
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by: Jan Drenth publisher: Springer, published: 2006-11-09 ASIN: 0387333347 EAN: 9780387333342 sales rank: 451712 X-ray crystallography is an established method for studying the structure of proteins and other macromolecules. As the importance of proteins grows, researchers in many fields have found that a working knowledge of X-ray diffraction is an indispensable tool. In this new edition of his essential work, the internationally recognized researcher Dr. Jan Drenth offers an up-to-date and technically rigorous introduction to the subject, providing the theoretical background necessary to understand how the structure of proteins is determined at atomic resolution. New material in the 3rd edition includes a section on twinning, an additional chapter on crystal growth and a discussion of single-wavelength anomalous dispersion. |
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publisher: Academic Press, published: 2003-01-02 ASIN: 0123617766 EAN: 9780123617767 sales rank: 1830980 This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins |
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by: Philip Bonner publisher: Taylor & Francis, published: 2007-04-11 ASIN: 0415385113 EAN: 9780415385114 sales rank: 2029988 Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification. |
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by: Robert K. Scopes publisher: Springer, published: 2010-12-06 ASIN: 1441928332 EAN: 9781441928337 sales rank: 2207705 The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" --Analytical Biochemistry "well organized and written clearly" --American Scientist "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" --Nature
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by: W.L.F. Armarego publisher: Butterworth-Heinemann, published: 2009-05-25 ASIN: 1856175677 EAN: 9781856175678 sales rank: 298413 A best seller since 1966, Purification of Laboratory Chemicals keeps engineers, scientists, chemists, biochemists and students up to date with the purification of the chemical reagents with which they work, the processes for their purification, and guides readerd on critical safety and hazards for the safe handling of chemicals and processes. The Sixth Edition is updated and provides expanded coverage of the latest chemical products and processing techniques, safety and hazards. The book has been reorganised and is now fully indexed by CAS Registry Numbers. Compounds are now grouped to make navigation easier and literature references for all substances and techniques have been added, and ambiguous alternate names and cross references have been removed. * The only comprehensive chemical purification reference, a market leader since 1966, Amarego delivers essential information for research and industrial chemists, pharmacists and engineers: '... (it) will be the most commonly used reference book in any chemical or biochemical laboratory' (MDPI Journal) * An essential lab practice and proceedures manual. Improves efficiency, results and safety by providing critical information for day-to-day lab and processing work. Improved, clear organization and new indexing delivers accurate, reliable information on processes and techniques of purification along with detailed physical properties. * The Sixth Edition has been reorganised and is fully indexed by CAS Registry Numbers; compounds are now grouped to make navigation easier; literature references for all substances and techniques have been added; ambiguous alternate names and cross references removed; new chemical products and processing techniques are covered; hazards and safety remain central to the book. |
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publisher: International University Line, published: 2009-04-15 ASIN: 0972077448 EAN: 9780972077446 sales rank: 1229250 Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization. Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The exercises have been tested in the Practical Protein Crystallization courses that have been running since 1994 at Uppsala University (Sweden), as well as courses elsewhere. The book is divided into four separate sections: Methods, Tools and Strategies, Laboratory Exercises, and an A-Z Glossary. There are 30 color plates for identifying phenomena found in crystallization drops. As in the first edition, the emphasis of the book is on practical problems and questions encountered at the lab bench. |